Restriction map for
pFN21AE3138
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# NO CUT ENZYMES ( 144 enzymes) #
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AatII Acc65I AclI AcyI AflII
AgeI AlwNI AocI Aor51HI ApaI
AscI AseI AsnI Asp700I Asp718I
AspI AviII AvrII BalI BamHI
BanI BbeI BbrPI BcgI BciVI
BfrI BglI BglII BlnI BlpI
Bpu1102I BsaAI BsaBI BsaHI BsaI
BsgI BsiEI BsiWI BsmI Bsp1407I
BspDI BspMI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstXI BstYI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DraI
DraIII DrdI DsaI EagI EclXI
Eco47III Eco52I Eco81I EcoO65I EcoRI
EcoRV EcoT22I FauI FseI FspI
HaeII HgaI Hin1I HindIII HpaI
KasI KpnI KspI MaeII MamI
MfeI MflI MluI MluNI MscI
MslI MunI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PstI PvuI
RsrII SacII SalI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI TaqI
Tsp45I Tth111I TthHB8I Van91I XbaI
XcmI XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AccI 1009 GTAGAC GT^[AC][GT]AC
AccIII 305 TCCGGA T^CCGGA
AcsI 430 AAATTT [GA]^AATT[CT]
AflIII 119 ACATGT A^C[GA][CT]GT
AhdI 34 GACAAGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 327 GTGCAC G^TGCAC
ApaLI 327 GTGCAC G^TGCAC
ApoI 430 AAATTT [GA]^AATT[CT]
AspEI 34 GACAAGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BanII 616 GAGCTC G[GA]GC[CT]^C
BbsI 414 GTCTTC GAAGAC(2/6)
BbvCI 820 GCTGAGG CCTCAGC(-5/-2)
BclI 225 TGATCA T^GATCA
BfaI 790 CTAG C^TAG
BpuAI 414 GTCTTC GAAGAC(2/6)
BsaWI 305 TCCGGA [AT]^CCGG[AT]
BseAI 305 TCCGGA T^CCGGA
BspEI 305 TCCGGA T^CCGGA
BspHI 522 TCATGA T^CATGA
BspLU11I 119 ACATGT A^CATGT
BsrBI 64 GAGCGG CCGCTC(-3/-3)
BstAPI 314 GCAGAGATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstNI 160 CCTGG CC^[AT]GG
Cac8I 337 GCCTGC GC[ACGT]^[ACGT]GC
CfoI 93 GCGC GCG^C
EaeI 67 CGGCCA [CT]^GGCC[GA]
Eam1105I 34 GACAAGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 535 CCTGTGTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRII 160 CCTGG ^CC[AT]GG
EcoT14I 896 CCTTGG C^C[AT][AT]GG
FbaI 225 TGATCA T^GATCA
HhaI 93 GCGC GCG^C
HinP1I 93 GCGC G^CGC
HincII 932 GTCAAC GT[CT]^[GA]AC
HindII 932 GTCAAC GT[CT]^[GA]AC
HphI 727 GGTGA GGTGA(8/7)
MaeI 790 CTAG C^TAG
MroI 305 TCCGGA T^CCGGA
MseI 905 TTAA T^TAA
MspA1I 81 CAGCTG C[AC]G^C[GT]G
MvaI 160 CCTGG CC^[AT]GG
NspI 119 ACATGT [GA]CATG^[CT]
PvuII 81 CAGCTG CAG^CTG
RcaI 522 TCATGA T^CATGA
SacI 616 GAGCTC GAGCT^C
SmaI 671 CCCGGG CCC^GGG
StyI 896 CCTTGG C^C[AT][AT]GG
TfiI 700 GAATC G^A[AT]TC
Tru9I 905 TTAA T^TAA
TspRI 126 CAGTG CA[GC]TG[ACGT][ACGT]^
XmaCI 671 CCCGGG C^CCGGG
XmaI 671 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 27 enzymes) #
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AccII 44 CGCG CG^CG
94 CGCG
AciI 45 GCGG CCGC(-3/-1)
66 GCGG
AlwI 106 GATCC GGATC(4/5)
87 GGATC
AspHI 327 GTGCAC G[AT]GC[AT]^C
616 GAGCTC
AvaI 49 CCCGAG C^[CT]CG[GA]G
671 CCCGGG
BcnI 671 CCCGG CC^[GC]GG
672 CCGGG
BmyI 327 GTGCAC G[AGT]GC[ACT]^C
616 GAGCTC
BpmI 555 CTGGAG CTGGAG(16/14)
1001 CTGGAG
BseRI 58 GAGGAG GAGGAG(10/8)
61 GAGGAG
BsiHKAI 327 GTGCAC G[AT]GC[AT]^C
616 GAGCTC
BsmBI 21 GAGACG CGTCTC(1/5)
991 GAGACG
BsoBI 49 CCCGAG C^[CT]CG[GA]G
671 CCCGGG
Bsp1286I 327 GTGCAC G[AGT]GC[ACT]^C
616 GAGCTC
BsrI 554 ACTGG ACTGG(1/-1)
471 CCAGT
BstUI 44 CGCG CG^CG
94 CGCG
DraII 279 GGGACCC [GA]G^G[ACGT]CC[CT]
882 AGGTCCT
EcoO109I 279 GGGACCC [GA]G^G[ACGT]CC[CT]
882 AGGTCCT
HaeIII 47 GGCC GG^CC
68 GGCC
HapII 306 CCGG C^CGG
672 CCGG
HpaII 306 CCGG C^CGG
672 CCGG
MspI 306 CCGG C^CGG
672 CCGG
MvnI 44 CGCG CG^CG
94 CGCG
NciI 671 CCCGG CC^[GC]GG
672 CCGGG
PleI 40 GAGTC GAGTC(4/5)
412 GAGTC
PpuMI 279 GGGACCC [GA]G^G[AT]CC[CT]
882 AGGTCCT
SapI 613 GAAGAGC GCTCTTC(1/4)
687 GAAGAGC
SfcI 236 CTACAG C^T[GA][CT]AG
920 CTGTAG
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# 3 OR MORE CUT ENZYMES ( 35 enzymes) #
#########################################
AfaI AluI AvaII BbvI BsaJI
BsiYI BslI BsmAI BsmFI BssKI
BstF5I Cfr13I DdeI DpnII EarI
Fnu4HI FokI HinfI ItaI Ksp632I
MaeIII MboI MboII MnlI MwoI
NdeII NlaIII NlaIV RsaI Sau3AI
Sau96I ScrFI SfaNI TseI Tsp509I