Restriction map for
pFN21AE4434
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# NO CUT ENZYMES ( 143 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcyI AgeI AhdI AocI Aor51HI
ApaI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AviII AvrII
BbeI BbrPI BbvCI BcgI BciVI
BclI BcnI BglI BlnI BlpI
Bpu1102I BsaBI BsaHI BsaI BsaWI
BseAI BsiEI BsiWI BsmBI BsmI
Bsp1407I BspDI BspEI BspHI BspMI
BsrFI BsrGI BssHII BstBI BstEII
BstPI BstUI Bsu36I CelII Cfr10I
ClaI CpoI DraII DraIII EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoRV EcoT22I FauI FbaI FseI
FspI HaeII HapII HgaI Hin1I
HincII HindII HpaI HpaII KasI
KpnI Ksp632I KspI MamI MfeI
MluI MroI MslI MspI MunI
MvnI NaeI NarI NciI NgoMIV
NheI NotI NruI NsiI NspV
PacI PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SapI SbfI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI Tth111I Van91I
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AccI 828 GTATAC GT^[AC][GT]AC
AcsI 232 GAATTT [GA]^AATT[CT]
AflII 1288 CTTAAG C^TTAAG
AflIII 867 ACATGT A^C[GA][CT]GT
Alw44I 935 GTGCAC G^TGCAC
AlwNI 694 CAGTTCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 935 GTGCAC G^TGCAC
ApoI 232 GAATTT [GA]^AATT[CT]
AspHI 935 GTGCAC G[AT]GC[AT]^C
AvaII 12 GGTCC G^G[AT]CC
BalI 143 TGGCCA TGG^CCA
BamHI 814 GGATCC G^GATCC
BfaI 200 CTAG C^TAG
BfrI 1288 CTTAAG C^TTAAG
BglII 287 AGATCT A^GATCT
BsaAI 1159 TACGTG [CT]AC^GT[GA]
BseRI 166 GAGGAG GAGGAG(10/8)
BsgI 792 CTGCAC GTGCAG(16/14)
BsiHKAI 935 GTGCAC G[AT]GC[AT]^C
BsmAI 1010 GAGAC GTCTC(1/5)
BsmFI 864 GGGAC GGGAC(10/14)
BspLU11I 867 ACATGT A^CATGT
BsrBI 416 GAGCGG CCGCTC(-3/-3)
BsrDI 489 CATTGC GCAATG(2/0)
BssSI 329 CACGAG CACGAG(-5/-1)
Bst1107I 828 GTATAC GTA^TAC
BstAPI 1024 GCATATGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 909 CCAGGAAATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 828 GTATAC GTA^TAC
CfoI 156 GCGC GCG^C
DrdI 576 GACTCTGAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 507 CCATGG C^C[GA][CT]GG
EaeI 143 TGGCCA [CT]^GGCC[GA]
HhaI 156 GCGC GCG^C
HinP1I 156 GCGC G^CGC
HindIII 1087 AAGCTT A^AGCTT
MaeI 200 CTAG C^TAG
MaeIII 1135 GTCAC ^GT[ACGT]AC
MluNI 143 TGGCCA TGG^CCA
MscI 143 TGGCCA TGG^CCA
MspA1I 676 CAGCTG C[AC]G^C[GT]G
NcoI 507 CCATGG C^CATGG
NdeI 1025 CATATG CA^TATG
NspI 867 ACATGT [GA]CATG^[CT]
PaeR7I 524 CTCGAG C^TCGAG
PstI 700 CTGCAG CTGCA^G
PvuII 676 CAGCTG CAG^CTG
SfcI 700 CTGCAG C^T[GA][CT]AG
TaqI 525 TCGA T^CGA
Tsp45I 1135 GTCAC ^GT[GC]AC
TthHB8I 525 TCGA T^CGA
XbaI 199 TCTAGA T^CTAGA
XhoI 524 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 13 enzymes) #
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AvaI 524 CTCGAG C^[CT]CG[GA]G
752 CTCGGG
BanI 97 GGTGCC G^G[CT][GA]CC
307 GGTGCC
BanII 994 GGGCTC G[GA]GC[CT]^C
1036 GGGCTC
BsoBI 524 CTCGAG C^[CT]CG[GA]G
752 CTCGGG
Cfr13I 12 GGTCC G^G[ACGT]CC
1223 GGCCC
DraI 213 TTTAAA TTT^AAA
445 TTTAAA
EcoT14I 507 CCATGG C^C[AT][AT]GG
1184 CCAAGG
HphI 1054 GGTGA GGTGA(8/7)
239 TCACC
MwoI 484 GCTGACATTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
1024 GCATATGGTGC
Sau96I 12 GGTCC G^G[ACGT]CC
1223 GGCCC
ScaI 644 AGTACT AGT^ACT
1064 AGTACT
SfaNI 427 GATGC GCATC(5/9)
1180 GATGC
StyI 507 CCATGG C^C[AT][AT]GG
1184 CCAAGG
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# 3 OR MORE CUT ENZYMES ( 49 enzymes) #
#########################################
AciI AfaI AluI AlwI BbsI
BbvI BmyI BpmI BpuAI BsaJI
BsiYI BslI Bsp1286I BsrI BssKI
BstF5I BstNI BstYI Cac8I DdeI
DpnII EcoRII Fnu4HI FokI HaeIII
HinfI ItaI MaeII MboI MboII
MflI MnlI MseI MvaI NdeII
NlaIII NlaIV PleI RsaI Sau3AI
ScrFI SmlI TfiI Tru9I TseI
Tsp509I TspRI XcmI XhoII