Restriction map for
pFN21AE2623
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# NO CUT ENZYMES ( 155 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AfaI AflII AflIII AgeI
AhdI Alw44I AlwI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvaI
AviII AvrII BamHI BbeI BbrPI
BbsI BcgI BciVI BclI BfrI
BglI BglII BlnI BlpI Bpu1102I
BpuAI BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BsgI BsiEI BsiWI
BsmBI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstYI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DraI DraII DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoRI
EcoRV EcoT14I FbaI FseI FspI
HaeII HgaI Hin1I HindIII KasI
KpnI KspI MaeII MamI MfeI
MflI MluI MroI MunI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RcaI RsaI RsrII SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SgrAI SmaI SnaBI SpeI
Sse8387I SspBI SspI StuI StyI
SwaI Tth111I Van91I XbaI XcmI
XhoI XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AccII 349 CGCG CG^CG
AcsI 566 AAATTC [GA]^AATT[CT]
AlwNI 167 CAGTTTCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 566 AAATTC [GA]^AATT[CT]
AspHI 453 GAGCTC G[AT]GC[AT]^C
BalI 620 TGGCCA TGG^CCA
BanI 160 GGTGCC G^G[CT][GA]CC
BanII 453 GAGCTC G[GA]GC[CT]^C
BbvCI 199 GCTGAGG CCTCAGC(-5/-2)
BcnI 24 CCGGG CC^[GC]GG
BmyI 453 GAGCTC G[AGT]GC[ACT]^C
BpmI 442 CTGGAG CTGGAG(16/14)
BsaJI 62 CCGTGG C^C[ACGT][ACGT]GG
BsiHKAI 453 GAGCTC G[AT]GC[AT]^C
BsmFI 7 GGGAC GGGAC(10/14)
BsmI 415 GCATTC GAATGC(1/-1)
Bsp1286I 453 GAGCTC G[AGT]GC[ACT]^C
BsrBI 614 CCGCTC CCGCTC(-3/-3)
BstBI 129 TTCGAA TT^CGAA
BstEII 27 GGTCACC G^GT[ACGT]ACC
BstNI 366 CCTGG CC^[AT]GG
BstPI 27 GGTCACC G^GT[ACGT]ACC
BstUI 349 CGCG CG^CG
BstXI 263 CCAAAGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 350 GCGC GCG^C
DsaI 62 CCGTGG C^C[GA][CT]GG
EaeI 620 TGGCCA [CT]^GGCC[GA]
EcoO65I 27 GGTCACC G^GT[ACGT]ACC
EcoRII 366 CCTGG ^CC[AT]GG
EcoT22I 413 ATGCAT ATGCA^T
FauI 20 CCCGC CCCGC(4/6)
HhaI 350 GCGC GCG^C
HinP1I 350 GCGC G^CGC
HincII 303 GTTAAC GT[CT]^[GA]AC
HindII 303 GTTAAC GT[CT]^[GA]AC
HpaI 303 GTTAAC GTT^AAC
MluNI 620 TGGCCA TGG^CCA
MscI 620 TGGCCA TGG^CCA
MseI 304 TTAA T^TAA
MvaI 366 CCTGG CC^[AT]GG
MvnI 349 CGCG CG^CG
NciI 24 CCGGG CC^[GC]GG
NsiI 413 ATGCAT ATGCA^T
NspI 662 GCATGC [GA]CATG^[CT]
NspV 129 TTCGAA TT^CGAA
PstI 556 CTGCAG CTGCA^G
SacI 453 GAGCTC GAGCT^C
SfuI 129 TTCGAA TT^CGAA
SmlI 667 CTTGAG C^T[CT][GA]AG
SphI 662 GCATGC GCATG^C
Tru9I 304 TTAA T^TAA
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AvaII 8 GGACC G^G[AT]CC
273 GGACC
BfaI 371 CTAG C^TAG
437 CTAG
BsmAI 135 GTCTC GTCTC(1/5)
534 GTCTC
BssKI 24 CCGGG ^CC[ACGT]GG
366 CCTGG
Cfr13I 8 GGACC G^G[ACGT]CC
273 GGACC
HaeIII 561 GGCC GG^CC
621 GGCC
HapII 24 CCGG C^CGG
78 CCGG
HpaII 24 CCGG C^CGG
78 CCGG
MaeI 371 CTAG C^TAG
437 CTAG
MslI 57 CATCACCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
60 CACCGTGGTG
MspI 24 CCGG C^CGG
78 CCGG
MwoI 154 GCTTATGGTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
399 GCTAAAGAAGC
NlaIII 649 CATG CATG^
663 CATG
NlaIV 7 GGGACC GG[ACGT]^[ACGT]CC
160 GGTGCC
PleI 470 GAGTC GAGTC(4/5)
532 GAGTC
PvuII 376 CAGCTG CAG^CTG
430 CAGCTG
Sau96I 8 GGACC G^G[ACGT]CC
273 GGACC
ScrFI 24 CCGGG CC^[ACGT]GG
366 CCTGG
SfaNI 412 GATGC GCATC(5/9)
74 GCATC
SfcI 465 CTACAG C^T[GA][CT]AG
556 CTGCAG
TfiI 112 GAATC G^A[AT]TC
232 GAATC
Tsp45I 28 GTCAC ^GT[GC]AC
472 GTCAC
Tsp509I 182 AATT ^AATT
567 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 29 enzymes) #
#########################################
AciI AluI BbvI BseRI BsiYI
BslI BsrI BstF5I Cac8I DdeI
DpnII EarI Fnu4HI FokI HinfI
HphI ItaI Ksp632I MaeIII MboI
MboII MnlI MspA1I NdeII Sau3AI
TaqI TseI TspRI TthHB8I