Restriction map for
pFN21AE4412
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# NO CUT ENZYMES ( 114 enzymes) #
##################################
AatII AccIII AclI AflII AflIII
AgeI AhdI Alw44I AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I AspEI AspI AviII AvrII
BbrPI BcgI BciVI BfaI BfrI
BglII BlnI BsaAI BsaBI BsaI
BsaWI BseAI BsgI BsiWI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrDI BsrGI BssHII BssSI Bst1107I
BstBI BstZ17I Bsu36I ClaI CpoI
DraI DraIII EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoRI
EcoT22I FspI HindIII HpaI KspI
MaeI MamI MfeI MluI MroI
MslI MunI NcoI NdeI NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI PvuII RcaI
RsrII SacII SalI SexAI SfiI
SfuI SgrAI SnaBI SpeI SphI
SspBI SspI StuI SwaI Tth111I
Van91I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 58 enzymes) #
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Acc65I 586 GGTACC G^GTACC
AccI 328 GTCTAC GT^[AC][GT]AC
AcsI 715 AAATTC [GA]^AATT[CT]
AcyI 130 GGCGCC G[GA]^CG[CT]C
AlwNI 1216 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 715 AAATTC [GA]^AATT[CT]
Asp718I 586 GGTACC G^GTACC
AvaI 1050 CCCGGG C^[CT]CG[GA]G
BamHI 1172 GGATCC G^GATCC
BbeI 130 GGCGCC GGCGC^C
BbvCI 810 CCTCAGC CCTCAGC(-5/-2)
BclI 47 TGATCA T^GATCA
BglI 671 GCCTTTCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 147 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 147 GCTGAGC GC^T[ACGT]AGC
BsaHI 130 GGCGCC G[GA]^CG[CT]C
BsiEI 753 CGACCG CG[GA][CT]^CG
BsmFI 311 GGGAC GGGAC(10/14)
BsoBI 1050 CCCGGG C^[CT]CG[GA]G
BspMI 539 ACCTGC ACCTGC(4/8)
BsrFI 1121 GCCGGC [GA]^CCGG[CT]
BstAPI 93 GCACTTCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1210 GGTTACC G^GT[ACGT]ACC
BstPI 1210 GGTTACC G^GT[ACGT]ACC
CelII 147 GCTGAGC GC^T[ACGT]AGC
Cfr10I 1121 GCCGGC [GA]^CCGG[CT]
DraII 1217 AGGCCCT [GA]G^G[ACGT]CC[CT]
DsaI 153 CCACGG C^C[GA][CT]GG
EcoO109I 1217 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 1210 GGTTACC G^GT[ACGT]ACC
EcoRV 939 GATATC GAT^ATC
EcoT14I 896 CCAAGG C^C[AT][AT]GG
FbaI 47 TGATCA T^GATCA
FseI 1120 GGCCGGCC GGCCGG^CC
HgaI 563 GCGTC GACGC(5/10)
Hin1I 130 GGCGCC G[GA]^CG[CT]C
HincII 535 GTCAAC GT[CT]^[GA]AC
HindII 535 GTCAAC GT[CT]^[GA]AC
KasI 130 GGCGCC G^GCGCC
KpnI 586 GGTACC GGTAC^C
NaeI 1121 GCCGGC GCC^GGC
NarI 130 GGCGCC GG^CGCC
NgoMIV 1121 GCCGGC G^CCGGC
PstI 391 CTGCAG CTGCA^G
SapI 654 GCTCTTC GCTCTTC(1/4)
SbfI 390 CCTGCAGG CCTGCA^GG
ScaI 1064 AGTACT AGT^ACT
SfcI 391 CTGCAG C^T[GA][CT]AG
SfoI 130 GGCGCC GGC^GCC
SmaI 1050 CCCGGG CCC^GGG
SmlI 598 CTCAAG C^T[CT][GA]AG
Sse8387I 390 CCTGCAGG CCTGCA^GG
StyI 896 CCAAGG C^C[AT][AT]GG
TfiI 977 GATTC G^A[AT]TC
Tsp509I 716 AATT ^AATT
XcmI 224 CCAATGCCTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 1050 CCCGGG C^CCGGG
XmaI 1050 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AccII 649 CGCG CG^CG
874 CGCG
AvaII 162 GGTCC G^G[AT]CC
1179 GGTCC
BbsI 16 GTCTTC GAAGAC(2/6)
511 GTCTTC
BpuAI 16 GTCTTC GAAGAC(2/6)
511 GTCTTC
BsmAI 427 GAGAC GTCTC(1/5)
1165 GTCTC
BsmBI 1164 CGTCTC CGTCTC(1/5)
427 GAGACG
BsmI 219 GAATGC GAATGC(1/-1)
617 GAATGC
BstUI 649 CGCG CG^CG
874 CGCG
BstXI 829 CCACTGATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
926 CCAACGGTGTGG
DrdI 313 GACAACTTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
429 GACGGGCAAGTC
EarI 655 CTCTTC CTCTTC(1/4)
1108 GAAGAG
Ksp632I 655 CTCTTC CTCTTC(1/4)
1108 GAAGAG
MaeII 29 ACGT A^CGT
1022 ACGT
MspA1I 115 CAGCGG C[AC]G^C[GT]G
142 CCGCTG
MvnI 649 CGCG CG^CG
874 CGCG
TaqI 214 TCGA T^CGA
659 TCGA
TthHB8I 214 TCGA T^CGA
659 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 69 enzymes) #
#########################################
AciI AfaI AluI AlwI AspHI
BalI BanI BanII BbvI BcnI
BmyI BpmI BsaJI BseRI BsiHKAI
BsiYI BslI Bsp1286I BsrI BssKI
BstF5I BstNI BstYI Cac8I CfoI
Cfr13I DdeI DpnII EaeI EcoRII
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PleI RsaI SacI
Sau3AI Sau96I ScrFI SfaNI Tru9I
TseI Tsp45I TspRI XhoII