Restriction map for
pFN21AE2303
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# NO CUT ENZYMES ( 72 enzymes) #
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AatII AccIII AflII AflIII AhdI
AscI Asp700I AspEI AspI BbeI
BfrI BglI BglII BlpI Bpu1102I
BseAI BsiEI BsiWI BspDI BspEI
BspLU11I BssHII BstAPI BstBI BstEII
BstPI CelII ClaI CpoI EagI
Eam1105I EclXI Eco52I EcoO65I EcoRV
FseI HpaI KasI KspI MfeI
MluI MroI MunI NaeI NarI
NgoMIV NotI NruI NspV PacI
PaeR7I PflFI PmeI PshAI PvuI
RsrII SacII SalI SbfI SfiI
SfoI SfuI SmaI SnaBI SpeI
Sse8387I SwaI Tth111I XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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Acc65I 2254 GGTACC G^GTACC
AccII 4396 CGCG CG^CG
AclI 1295 AACGTT AA^CGTT
AgeI 4755 ACCGGT A^CCGGT
AocI 3628 CCTCAGG CC^T[ACGT]AGG
AseI 6123 ATTAAT AT^TAAT
AsnI 6123 ATTAAT AT^TAAT
Asp718I 2254 GGTACC G^GTACC
AvaI 2776 CCCGAG C^[CT]CG[GA]G
AviII 4506 TGCGCA TGC^GCA
AvrII 1005 CCTAGG C^CTAGG
BalI 5712 TGGCCA TGG^CCA
BbrPI 6537 CACGTG CAC^GTG
BbvCI 1681 CCTCAGC CCTCAGC(-5/-2)
BcgI 1899 GCATAGTTATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 5738 GTATCC GTATCC(6/5)
BclI 572 TGATCA T^GATCA
BlnI 1005 CCTAGG C^CTAGG
BsaBI 3357 GATGAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 4755 ACCGGT [AT]^CCGG[AT]
BsoBI 2776 CCCGAG C^[CT]CG[GA]G
BssSI 1688 CACGAG CACGAG(-5/-1)
Bst1107I 4330 GTATAC GTA^TAC
BstUI 4396 CGCG CG^CG
BstZ17I 4330 GTATAC GTA^TAC
Bsu36I 3628 CCTCAGG CC^T[ACGT]AGG
Eco81I 3628 CCTCAGG CC^T[ACGT]AGG
FbaI 572 TGATCA T^GATCA
FspI 4506 TGCGCA TGC^GCA
HindIII 1285 AAGCTT A^AGCTT
KpnI 2254 GGTACC GGTAC^C
MamI 3357 GATGAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 5712 TGGCCA TGG^CCA
MscI 5712 TGGCCA TGG^CCA
MvnI 4396 CGCG CG^CG
NheI 1216 GCTAGC G^CTAGC
PinAI 4755 ACCGGT A^CCGGT
PmaCI 6537 CACGTG CAC^GTG
PmlI 6537 CACGTG CAC^GTG
PshBI 6123 ATTAAT AT^TAAT
Psp1406I 1295 AACGTT AA^CGTT
ScaI 624 AGTACT AGT^ACT
SgrAI 2288 CGCCGGTG C[GA]^CCGG[CT]G
SphI 3947 GCATGC GCATG^C
SspI 5569 AATATT AAT^ATT
XbaI 6247 TCTAGA T^CTAGA
XcmI 909 CCACATTGCAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 28 enzymes) #
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AccI 1776 GTCTAC GT^[AC][GT]AC
4330 GTATAC
AcyI 215 GGCGTC G[GA]^CG[CT]C
5705 GGCGTC
Alw44I 2803 GTGCAC G^TGCAC
3910 GTGCAC
Aor51HI 3076 AGCGCT AGC^GCT
5507 AGCGCT
ApaI 1309 GGGCCC GGGCC^C
4636 GGGCCC
ApaLI 2803 GTGCAC G^TGCAC
3910 GTGCAC
BamHI 1245 GGATCC G^GATCC
2836 GGATCC
BsaHI 215 GGCGTC G[GA]^CG[CT]C
5705 GGCGTC
BsmBI 1909 CGTCTC CGTCTC(1/5)
5440 GAGACG
Bsp1407I 5977 TGTACA T^GTACA
6605 TGTACA
BspHI 297 TCATGA T^CATGA
6617 TCATGA
BsrBI 4464 GAGCGG CCGCTC(-3/-3)
5362 GAGCGG
BsrFI 2289 GCCGGT [GA]^CCGG[CT]
4755 ACCGGT
BsrGI 5977 TGTACA T^GTACA
6605 TGTACA
Cfr10I 2289 GCCGGT [GA]^CCGG[CT]
4755 ACCGGT
DraIII 1916 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
6583 CACTTTGTG
DrdI 1789 GACAGAACTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5699 GACTTGGGCGTC
Eco47III 3076 AGCGCT AGC^GCT
5507 AGCGCT
EcoNI 2388 CCTTACACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3624 CCTTCCTCAGG
EcoT22I 1149 ATGCAT ATGCA^T
1491 ATGCAT
FauI 3289 CCCGC CCCGC(4/6)
6453 GCGGG
Hin1I 215 GGCGTC G[GA]^CG[CT]C
5705 GGCGTC
NsiI 1149 ATGCAT ATGCA^T
1491 ATGCAT
RcaI 297 TCATGA T^CATGA
6617 TCATGA
SacI 1948 GAGCTC GAGCT^C
4920 GAGCTC
SexAI 1043 ACCTGGT A^CC[AT]GGT
2274 ACCAGGT
SspBI 5977 TGTACA T^GTACA
6605 TGTACA
StuI 1939 AGGCCT AGG^CCT
4283 AGGCCT
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# 3 OR MORE CUT ENZYMES ( 111 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AspHI AvaII BanI
BanII BbsI BbvI BcnI BfaI
BmyI BpmI BpuAI BsaAI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI Bsp1286I
BspMI BsrDI BsrI BssKI BstF5I
BstNI BstXI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraI DraII
DsaI EaeI EarI EcoO109I EcoRI
EcoRII EcoT14I Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MwoI
NciI NcoI NdeI NdeII NlaIII
NlaIV NspI PflMI PleI PpuMI
PstI PvuII RsaI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XhoII