Restriction map for
pFN21AA0564
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# NO CUT ENZYMES ( 55 enzymes) #
##################################
AatII AcyI AgeI AscI AspI
AviII BalI BbeI BsaHI BsiWI
Bsp1407I BspDI BspLU11I BsrBI BsrGI
BssHII BssSI BstXI ClaI CpoI
FseI FspI Hin1I HpaI KasI
KspI MluI MluNI MscI NarI
NdeI NheI NotI NruI PacI
PflFI PinAI PmeI PshAI PvuI
RsrII SacII SapI SbfI SfoI
SgrAI SmaI SnaBI SpeI Sse8387I
SspBI SwaI Tth111I XmaCI XmaI
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# ONE CUT ENZYMES ( 53 enzymes) #
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Acc65I 2749 GGTACC G^GTACC
AccIII 1181 TCCGGA T^CCGGA
AclI 3135 AACGTT AA^CGTT
AflII 823 CTTAAG C^TTAAG
Aor51HI 1079 AGCGCT AGC^GCT
ApaI 3599 GGGCCC GGGCC^C
AseI 3331 ATTAAT AT^TAAT
AsnI 3331 ATTAAT AT^TAAT
Asp718I 2749 GGTACC G^GTACC
AvrII 2730 CCTAGG C^CTAGG
BamHI 1938 GGATCC G^GATCC
BbvCI 2883 GCTGAGG CCTCAGC(-5/-2)
BcgI 393 CGAATACATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 823 CTTAAG C^TTAAG
BlnI 2730 CCTAGG C^CTAGG
BsaWI 1181 TCCGGA [AT]^CCGG[AT]
BseAI 1181 TCCGGA T^CCGGA
BsiEI 118 CGGCCG CG[GA][CT]^CG
BsmBI 3610 CGTCTC CGTCTC(1/5)
BspEI 1181 TCCGGA T^CCGGA
BspHI 1044 TCATGA T^CATGA
Bst1107I 4428 GTATAC GTA^TAC
BstBI 2996 TTCGAA TT^CGAA
BstEII 1200 GGTGACC G^GT[ACGT]ACC
BstPI 1200 GGTGACC G^GT[ACGT]ACC
BstZ17I 4428 GTATAC GTA^TAC
DrdI 4541 GACTTGAACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 118 CGGCCG C^GGCCG
EclXI 118 CGGCCG C^GGCCG
Eco47III 1079 AGCGCT AGC^GCT
Eco52I 118 CGGCCG C^GGCCG
EcoO65I 1200 GGTGACC G^GT[ACGT]ACC
EcoT22I 5374 ATGCAT ATGCA^T
HaeII 1079 AGCGCT [GA]GCGC^[CT]
KpnI 2749 GGTACC GGTAC^C
MroI 1181 TCCGGA T^CCGGA
NcoI 5648 CCATGG C^CATGG
NsiI 5374 ATGCAT ATGCA^T
NspV 2996 TTCGAA TT^CGAA
PaeR7I 2809 CTCGAG C^TCGAG
PshBI 3331 ATTAAT AT^TAAT
Psp1406I 3135 AACGTT AA^CGTT
PstI 4552 CTGCAG CTGCA^G
RcaI 1044 TCATGA T^CATGA
SacI 2806 GAGCTC GAGCT^C
SalI 4443 GTCGAC G^TCGAC
SexAI 4790 ACCAGGT A^CC[AT]GGT
SfiI 37 GGCCACGGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 2996 TTCGAA TT^CGAA
SspI 175 AATATT AAT^ATT
StuI 4737 AGGCCT AGG^CCT
XbaI 3765 TCTAGA T^CTAGA
XhoI 2809 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 33 enzymes) #
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AccI 4428 GTATAC GT^[AC][GT]AC
4443 GTCGAC
AccII 56 CGCG CG^CG
1436 CGCG
AflIII 2569 ACGTGT A^C[GA][CT]GT
5411 ACACGT
AhdI 2973 GACTGAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3153 GACAATTGGTC
Alw44I 2210 GTGCAC G^TGCAC
4951 GTGCAC
AlwNI 1777 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG
4931 CAGGTGCTG
ApaLI 2210 GTGCAC G^TGCAC
4951 GTGCAC
AspEI 2973 GACTGAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3153 GACAATTGGTC
BbrPI 2568 CACGTG CAC^GTG
4687 CACGTG
BglI 38 GCCACGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
4739 GCCTGGGTGGC
BglII 4122 AGATCT A^GATCT
5684 AGATCT
BlpI 625 GCTGAGC GC^T[ACGT]AGC
1605 GCTAAGC
Bpu1102I 625 GCTGAGC GC^T[ACGT]AGC
1605 GCTAAGC
BsaAI 2568 CACGTG [CT]AC^GT[GA]
4687 CACGTG
BspMI 2387 ACCTGC ACCTGC(4/8)
81 GCAGGT
BstUI 56 CGCG CG^CG
1436 CGCG
CelII 625 GCTGAGC GC^T[ACGT]AGC
1605 GCTAAGC
DraIII 1549 CACCGGGTG CAC[ACGT][ACGT][ACGT]^GTG
4133 CACCCAGTG
DsaI 39 CCACGG C^C[GA][CT]GG
5648 CCATGG
Eam1105I 2973 GACTGAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3153 GACAATTGGTC
EcoRI 1792 GAATTC G^AATTC
4073 GAATTC
FauI 3403 CCCGC CCCGC(4/6)
1561 GCGGG
HindIII 1166 AAGCTT A^AGCTT
2860 AAGCTT
MvnI 56 CGCG CG^CG
1436 CGCG
NaeI 142 GCCGGC GCC^GGC
5198 GCCGGC
NgoMIV 142 GCCGGC G^CCGGC
5198 GCCGGC
PflMI 1776 CCAGTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5107 CCACAACTTGG
PmaCI 2568 CACGTG CAC^GTG
4687 CACGTG
PmlI 2568 CACGTG CAC^GTG
4687 CACGTG
ScaI 365 AGTACT AGT^ACT
5468 AGTACT
SphI 62 GCATGC GCATG^C
5376 GCATGC
Van91I 1776 CCAGTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5107 CCACAACTTGG
XcmI 659 CCAAAAAAGAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3095 CCAGTGTGCAGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 117 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AocI ApoI Asp700I AspHI AvaI
AvaII BanI BanII BbsI BbvI
BciVI BclI BcnI BfaI BmyI
BpmI BpuAI BsaBI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
BsrDI BsrFI BsrI BssKI BstAPI
BstF5I BstNI BstYI Bsu36I Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraI DraII EaeI EarI Eco81I
EcoNI EcoO109I EcoRII EcoRV EcoT14I
FbaI Fnu4HI FokI HaeIII HapII
HgaI HhaI HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MamI MboI
MboII MfeI MflI MnlI MseI
MslI MspA1I MspI MunI MvaI
MwoI NciI NdeII NlaIII NlaIV
NspI PleI PpuMI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII XmnI