Restriction map for
pFN21AE1253
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# NO CUT ENZYMES ( 114 enzymes) #
##################################
AatII AccII AccIII AclI AflII
AgeI Alw44I AocI Aor51HI ApaLI
AscI AseI AsnI AspI AviII
AvrII BalI BamHI BbrPI BbvCI
BcgI BciVI BclI BfrI BlnI
BlpI Bpu1102I BsaAI BsaBI BsaWI
BseAI BseRI BsgI BsiEI BsiWI
BsmBI Bsp1407I BspDI BspEI BspHI
BsrBI BsrDI BsrGI BssHII BssSI
Bst1107I BstBI BstUI BstZ17I Bsu36I
CelII ClaI CpoI DraI DrdI
EagI EclXI Eco47III Eco52I Eco81I
EcoNI EcoRI EcoT22I FauI FbaI
FseI FspI KspI MamI MfeI
MluI MluNI MroI MscI MslI
MunI MvnI NcoI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
PvuII RcaI RsrII SacII SapI
SbfI ScaI SexAI SfiI SfuI
SgrAI SnaBI SpeI Sse8387I SspBI
SwaI Tth111I XbaI XhoI
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# ONE CUT ENZYMES ( 54 enzymes) #
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Acc65I 1369 GGTACC G^GTACC
AccI 145 GTCGAC GT^[AC][GT]AC
AcyI 780 GGCGCC G[GA]^CG[CT]C
AhdI 938 GACCTCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 1541 GATCC GGATC(4/5)
AlwNI 286 CAGTGCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 401 GGGCCC GGGCC^C
Asp700I 840 GAAGAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1369 GGTACC G^GTACC
AspEI 938 GACCTCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbeI 780 GGCGCC GGCGC^C
BbsI 1189 GAAGAC GAAGAC(2/6)
BfaI 1070 CTAG C^TAG
BglI 527 GCCTTCCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 604 AGATCT A^GATCT
BpuAI 1189 GAAGAC GAAGAC(2/6)
BsaHI 780 GGCGCC G[GA]^CG[CT]C
BsmI 747 GAATGC GAATGC(1/-1)
BsrFI 783 GCCGGC [GA]^CCGG[CT]
BstEII 1059 GGTCACC G^GT[ACGT]ACC
BstPI 1059 GGTCACC G^GT[ACGT]ACC
Cfr10I 783 GCCGGC [GA]^CCGG[CT]
DraIII 1267 CACTTGGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 630 CCACGG C^C[GA][CT]GG
Eam1105I 938 GACCTCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 1059 GGTCACC G^GT[ACGT]ACC
EcoRV 676 GATATC GAT^ATC
HaeII 780 GGCGCC [GA]GCGC^[CT]
HgaI 354 GCGTC GACGC(5/10)
Hin1I 780 GGCGCC G[GA]^CG[CT]C
HpaI 798 GTTAAC GTT^AAC
KasI 780 GGCGCC G^GCGCC
KpnI 1369 GGTACC GGTAC^C
MaeI 1070 CTAG C^TAG
MseI 799 TTAA T^TAA
MspA1I 97 CAGCGG C[AC]G^C[GT]G
NaeI 783 GCCGGC GCC^GGC
NarI 780 GGCGCC GG^CGCC
NgoMIV 783 GCCGGC G^CCGGC
PflMI 246 CCAGAATATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 283 CTGCAG CTGCA^G
SacI 1219 GAGCTC GAGCT^C
SalI 145 GTCGAC G^TCGAC
SfcI 283 CTGCAG C^T[GA][CT]AG
SfoI 780 GGCGCC GGC^GCC
SmaI 329 CCCGGG CCC^GGG
SspI 585 AATATT AAT^ATT
StuI 1097 AGGCCT AGG^CCT
Tru9I 799 TTAA T^TAA
Van91I 246 CCAGAATATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 579 CCACCAAATATTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 329 CCCGGG C^CCGGG
XmaI 329 CCCGGG C^CCGGG
XmnI 840 GAAGAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AcsI 487 GAATTT [GA]^AATT[CT]
1312 AAATTC
AflIII 346 ACATGT A^C[GA][CT]GT
917 ACATGT
ApoI 487 GAATTT [GA]^AATT[CT]
1312 AAATTC
AvaI 329 CCCGGG C^[CT]CG[GA]G
1252 CCCGAG
BanI 780 GGCGCC G^G[CT][GA]CC
1369 GGTACC
BsaI 219 GAGACC GGTCTC(1/5)
1229 GAGACC
BsmFI 327 GTCCC GGGAC(10/14)
356 GTCCC
BsoBI 329 CCCGGG C^[CT]CG[GA]G
1252 CCCGAG
BspLU11I 346 ACATGT A^CATGT
917 ACATGT
BstAPI 79 GCAGCTTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
442 GCATTGGCTGC
BstXI 408 CCACAGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
582 CCAAATATTTGG
BstYI 604 AGATCT [GA]^GATC[CT]
1540 AGATCC
CfoI 781 GCGC GCG^C
1184 GCGC
EaeI 70 CGGCCA [CT]^GGCC[GA]
534 CGGCCA
EcoT14I 818 CCAAGG C^C[AT][AT]GG
1179 CCTTGG
HhaI 781 GCGC GCG^C
1184 GCGC
HinP1I 781 GCGC G^CGC
1184 GCGC
HindIII 199 AAGCTT A^AGCTT
704 AAGCTT
MflI 604 AGATCT [GA]^GATC[CT]
1540 AGATCC
PpuMI 821 AGGTCCT [GA]G^G[AT]CC[CT]
936 AGGACCT
SmlI 1022 CTCAAG C^T[CT][GA]AG
1222 CTCAAG
SphI 368 GCATGC GCATG^C
523 GCATGC
StyI 818 CCAAGG C^C[AT][AT]GG
1179 CCTTGG
Tsp509I 488 AATT ^AATT
1313 AATT
XhoII 604 AGATCT [GA]^GATC[CT]
1540 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 65 enzymes) #
#########################################
AciI AfaI AluI AspHI AvaII
BanII BbvI BcnI BmyI BpmI
BsaJI BsiHKAI BsiYI BslI BsmAI
Bsp1286I BspMI BsrI BssKI BstF5I
BstNI Cac8I Cfr13I DdeI DpnII
DraII EarI EcoO109I EcoRII Fnu4HI
FokI HaeIII HapII HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeII MaeIII MboI MboII MnlI
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PleI RsaI
Sau3AI Sau96I ScrFI SfaNI TaqI
TfiI TseI Tsp45I TspRI TthHB8I