Restriction map for
pFN21AE9450
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# NO CUT ENZYMES ( 60 enzymes) #
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Acc65I AccI AclI AflII AscI
AseI AsnI Asp700I Asp718I AvrII
BcgI BclI BfaI BfrI BlnI
BsaBI BsiWI Bsp1407I BsrGI Bst1107I
BstBI BstZ17I CpoI DraI EcoRV
FbaI FseI HindIII HpaI KpnI
MaeI MamI MfeI MseI MunI
NcoI NdeI NheI NruI NspV
PacI PmeI PshBI Psp1406I PvuI
RsrII SalI SapI ScaI SfuI
SgrAI SnaBI SpeI SphI SspBI
SspI SwaI Tru9I XbaI XmnI
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# ONE CUT ENZYMES ( 32 enzymes) #
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AatII 2248 GACGTC GACGT^C
AcsI 2544 GAATTC [GA]^AATT[CT]
AgeI 1054 ACCGGT A^CCGGT
AhdI 2044 GACCTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 3152 AGCGCT AGC^GCT
ApoI 2544 GAATTC [GA]^AATT[CT]
AspEI 2044 GACCTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2407 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbvCI 4137 CCTCAGC CCTCAGC(-5/-2)
BspDI 2059 ATCGAT AT^CGAT
BspHI 3662 TCATGA T^CATGA
BsrDI 1371 CATTGC GCAATG(2/0)
BssHII 80 GCGCGC G^CGCGC
BstEII 1057 GGTGACC G^GT[ACGT]ACC
BstPI 1057 GGTGACC G^GT[ACGT]ACC
BstXI 3197 CCATTTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
ClaI 2059 ATCGAT AT^CGAT
Eam1105I 2044 GACCTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 3152 AGCGCT AGC^GCT
EcoO65I 1057 GGTGACC G^GT[ACGT]ACC
EcoRI 2544 GAATTC G^AATTC
EcoT22I 2282 ATGCAT ATGCA^T
HincII 3598 GTTGAC GT[CT]^[GA]AC
HindII 3598 GTTGAC GT[CT]^[GA]AC
NsiI 2282 ATGCAT ATGCA^T
PaeR7I 322 CTCGAG C^TCGAG
PflFI 2407 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 1054 ACCGGT A^CCGGT
RcaI 3662 TCATGA T^CATGA
SfiI 4146 GGCCCCCGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
Tth111I 2407 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 322 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 36 enzymes) #
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Alw44I 3030 GTGCAC G^TGCAC
3547 GTGCAC
AocI 2067 CCTGAGG CC^T[ACGT]AGG
2274 CCTGAGG
ApaLI 3030 GTGCAC G^TGCAC
3547 GTGCAC
AviII 296 TGCGCA TGC^GCA
2591 TGCGCA
BbrPI 1608 CACGTG CAC^GTG
4623 CACGTG
BbsI 2431 GTCTTC GAAGAC(2/6)
3511 GTCTTC
BciVI 2651 GTATCC GTATCC(6/5)
3554 GTATCC
BglII 1736 AGATCT A^GATCT
2012 AGATCT
BpuAI 2431 GTCTTC GAAGAC(2/6)
3511 GTCTTC
BsmBI 3767 CGTCTC CGTCTC(1/5)
2665 GAGACG
BsmI 814 GAATGC GAATGC(1/-1)
1477 GAATGC
BspLU11I 2621 ACATGT A^CATGT
4481 ACATGT
BssSI 4026 CACGAG CACGAG(-5/-1)
3349 CTCGTG
Bsu36I 2067 CCTGAGG CC^T[ACGT]AGG
2274 CCTGAGG
DraIII 1161 CACGCAGTG CAC[ACGT][ACGT][ACGT]^GTG
1519 CACTGGGTG
DrdI 2832 GACTCTGCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3444 GACCTGGATGTC
EarI 3490 CTCTTC CTCTTC(1/4)
3502 CTCTTC
Eco81I 2067 CCTGAGG CC^T[ACGT]AGG
2274 CCTGAGG
EcoNI 750 CCTGACCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1947 CCTGGCCAAGG
FspI 296 TGCGCA TGC^GCA
2591 TGCGCA
Ksp632I 3490 CTCTTC CTCTTC(1/4)
3502 CTCTTC
MluI 581 ACGCGT A^CGCGT
1471 ACGCGT
NaeI 3837 GCCGGC GCC^GGC
4299 GCCGGC
NgoMIV 3837 GCCGGC G^CCGGC
4299 GCCGGC
NotI 88 GCGGCCGC GC^GGCCGC
169 GCGGCCGC
NspI 2621 ACATGT [GA]CATG^[CT]
4481 ACATGT
PmaCI 1608 CACGTG CAC^GTG
4623 CACGTG
PmlI 1608 CACGTG CAC^GTG
4623 CACGTG
PshAI 580 GACGCGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
4743 GACCGAGGTC
PvuII 1443 CAGCTG CAG^CTG
2194 CAGCTG
SacI 3411 GAGCTC GAGCT^C
4401 GAGCTC
SbfI 405 CCTGCAGG CCTGCA^GG
1304 CCTGCAGG
Sse8387I 405 CCTGCAGG CCTGCA^GG
1304 CCTGCAGG
TfiI 2169 GAATC G^A[AT]TC
3209 GATTC
Tsp509I 2545 AATT ^AATT
3823 AATT
XcmI 2619 CCACATGTATAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4703 CCACGATCCCGCTGG
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# 3 OR MORE CUT ENZYMES ( 130 enzymes) #
#########################################
AccII AccIII AciI AcyI AfaI
AflIII AluI AlwI AlwNI ApaI
AspHI AvaI AvaII BalI BamHI
BanI BanII BbeI BbvI BcnI
BglI BlpI BmyI BpmI Bpu1102I
BsaAI BsaHI BsaI BsaJI BsaWI
BseAI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspEI BspMI BsrBI BsrFI
BsrI BssKI BstAPI BstF5I BstNI
BstUI BstYI Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EagI EclXI Eco52I
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI KasI KspI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MroI MscI MslI MspA1I
MspI MvaI MvnI MwoI NarI
NciI NdeII NlaIII NlaIV PflMI
PleI PpuMI PstI RsaI SacII
Sau3AI Sau96I ScrFI SexAI SfaNI
SfcI SfoI SmaI SmlI StuI
StyI TaqI TseI Tsp45I TspRI
TthHB8I Van91I XhoII XmaCI XmaI