Restriction map for
pFN21AE5722
##################################
# NO CUT ENZYMES ( 117 enzymes) #
##################################
AatII Acc65I AccII AclI AcyI
AflII AflIII AgeI AocI Aor51HI
AscI AseI AsnI Asp700I Asp718I
AspI AvaI AviII BalI BanI
BbeI BbrPI BbvCI BcgI BciVI
BfrI BglI BglII BlpI Bpu1102I
BsaAI BsaBI BsaHI BsiWI BsmI
BsoBI Bsp1407I BspHI BspLU11I BspMI
BsrBI BsrGI BssHII BstEII BstPI
BstUI Bsu36I CelII CfoI CpoI
DraIII DrdI Eco47III Eco81I EcoO65I
EcoT22I FseI FspI HaeII HhaI
Hin1I HinP1I HpaI KasI KpnI
KspI MamI MfeI MluI MluNI
MscI MunI MvnI NarI NdeI
NheI NotI NruI NsiI PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SbfI ScaI SexAI SfiI
SfoI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SwaI
Tth111I Van91I XbaI XhoI XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AccI 2315 GTATAC GT^[AC][GT]AC
AhdI 428 GACTCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 20 GTGCAC G^TGCAC
AlwNI 1448 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 2059 GGGCCC GGGCC^C
ApaLI 20 GTGCAC G^TGCAC
AspEI 428 GACTCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 1149 CCTAGG C^CTAGG
BamHI 1585 GGATCC G^GATCC
BclI 75 TGATCA T^GATCA
BlnI 1149 CCTAGG C^CTAGG
BsiEI 2041 CGGCCG CG[GA][CT]^CG
BsmBI 1236 CGTCTC CGTCTC(1/5)
BspDI 47 ATCGAT AT^CGAT
BsrDI 2373 CATTGC GCAATG(2/0)
Bst1107I 2315 GTATAC GTA^TAC
BstAPI 1447 GCAGCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 1869 TTCGAA TT^CGAA
BstZ17I 2315 GTATAC GTA^TAC
ClaI 47 ATCGAT AT^CGAT
EagI 2041 CGGCCG C^GGCCG
Eam1105I 428 GACTCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 2041 CGGCCG C^GGCCG
Eco52I 2041 CGGCCG C^GGCCG
EcoNI 1972 CCTGAACGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FauI 453 GCGGG CCCGC(4/6)
FbaI 75 TGATCA T^GATCA
MspA1I 1448 CAGCTG C[AC]G^C[GT]G
NaeI 2239 GCCGGC GCC^GGC
NcoI 165 CCATGG C^CATGG
NgoMIV 2239 GCCGGC G^CCGGC
NspV 1869 TTCGAA TT^CGAA
PstI 410 CTGCAG CTGCA^G
PvuII 1448 CAGCTG CAG^CTG
SapI 2563 GAAGAGC GCTCTTC(1/4)
SfaNI 1432 GCATC GCATC(5/9)
SfuI 1869 TTCGAA TT^CGAA
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AfaI 978 GTAC GT^AC
2247 GTAC
BcnI 1577 CCGGG CC^[GC]GG
2039 CCCGG
BfaI 1150 CTAG C^TAG
2476 CTAG
BsmFI 265 GGGAC GGGAC(10/14)
343 GGGAC
BsrFI 2043 GCCGGT [GA]^CCGG[CT]
2239 GCCGGC
BssSI 1438 CACGAG CACGAG(-5/-1)
121 CTCGTG
BstXI 1283 CCACTGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2393 CCAGGTCTCTGG
BstYI 1397 AGATCC [GA]^GATC[CT]
1585 GGATCC
Cfr10I 2043 GCCGGT [GA]^CCGG[CT]
2239 GCCGGC
DraI 819 TTTAAA TTT^AAA
1684 TTTAAA
DraII 594 AGGCCCT [GA]G^G[ACGT]CC[CT]
2058 GGGGCCC
DsaI 165 CCATGG C^C[GA][CT]GG
1425 CCGTGG
EaeI 2041 CGGCCG [CT]^GGCC[GA]
2241 CGGCCA
EarI 775 GAAGAG CTCTTC(1/4)
2563 GAAGAG
EcoO109I 594 AGGCCCT [GA]G^G[ACGT]CC[CT]
2058 GGGGCCC
EcoRI 312 GAATTC G^AATTC
2098 GAATTC
HgaI 64 GACGC GACGC(5/10)
2427 GCGTC
HincII 226 GTCAAC GT[CT]^[GA]AC
1006 GTTGAC
HindII 226 GTCAAC GT[CT]^[GA]AC
1006 GTTGAC
HindIII 1113 AAGCTT A^AGCTT
2551 AAGCTT
Ksp632I 775 GAAGAG CTCTTC(1/4)
2563 GAAGAG
MaeI 1150 CTAG C^TAG
2476 CTAG
MflI 1397 AGATCC [GA]^GATC[CT]
1585 GGATCC
MslI 166 CATGGATATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
843 CATTTATATG
NciI 1577 CCGGG CC^[GC]GG
2039 CCCGG
RsaI 978 GTAC GT^AC
2247 GTAC
SacI 1378 GAGCTC GAGCT^C
1572 GAGCTC
SfcI 410 CTGCAG C^T[GA][CT]AG
750 CTACAG
SspI 789 AATATT AAT^ATT
2495 AATATT
StuI 1024 AGGCCT AGG^CCT
1152 AGGCCT
Tsp45I 1106 GTGAC ^GT[GC]AC
1480 GTGAC
XhoII 1397 AGATCC [GA]^GATC[CT]
1585 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 72 enzymes) #
#########################################
AccIII AciI AcsI AluI AlwI
ApoI AspHI AvaII BanII BbsI
BbvI BmyI BpmI BpuAI BsaI
BsaJI BsaWI BseAI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI Bsp1286I
BspEI BsrI BssKI BstF5I BstNI
Cac8I Cfr13I DdeI DpnII EcoRII
EcoRV EcoT14I Fnu4HI FokI HaeIII
HapII HinfI HpaII HphI ItaI
MaeII MaeIII MboI MboII MnlI
MroI MseI MspI MvaI MwoI
NdeII NlaIII NlaIV NspI PleI
Sau3AI Sau96I ScrFI StyI TaqI
TfiI Tru9I TseI Tsp509I TspRI
TthHB8I XcmI