Restriction map for
pFN21AE3957
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# NO CUT ENZYMES ( 160 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AcsI AcyI AflII
AgeI AhdI Alw44I Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp718I AspEI AspI AvaI AviII
AvrII BalI BamHI BanI BbeI
BbrPI BbvCI BcgI BciVI BclI
BfaI BfrI BglI BglII BlnI
BlpI Bpu1102I BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BsiEI BsiWI
BsmBI BsmFI BsoBI Bsp1407I BspDI
BspEI BspHI BspMI BsrBI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstYI BstZ17I CelII CfoI Cfr10I
ClaI CpoI DraI DraIII DrdI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I EcoO65I EcoRI EcoRV EcoT22I
FauI FbaI FseI FspI HaeII
HhaI Hin1I HinP1I HindIII HpaI
KasI KpnI KspI MaeI MaeII
MamI MfeI MflI MluI MluNI
MroI MscI MunI MvnI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI Tsp509I Tth111I
XbaI XhoI XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AflIII 126 ACATGT A^C[GA][CT]GT
AlwI 592 GATCC GGATC(4/5)
AocI 63 CCTGAGG CC^T[ACGT]AGG
Asp700I 315 GAACTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbsI 681 GAAGAC GAAGAC(2/6)
BcnI 198 CCGGG CC^[GC]GG
BpmI 34 CTGGAG CTGGAG(16/14)
BpuAI 681 GAAGAC GAAGAC(2/6)
BsgI 163 CTGCAC GTGCAG(16/14)
BsmI 290 GCATTC GAATGC(1/-1)
BspLU11I 126 ACATGT A^CATGT
Bsu36I 63 CCTGAGG CC^T[ACGT]AGG
DraII 432 AGGTCCT [GA]G^G[ACGT]CC[CT]
DsaI 134 CCATGG C^C[GA][CT]GG
EarI 202 GAAGAG CTCTTC(1/4)
Eco81I 63 CCTGAGG CC^T[ACGT]AGG
EcoO109I 432 AGGTCCT [GA]G^G[ACGT]CC[CT]
HapII 198 CCGG C^CGG
HgaI 554 GCGTC GACGC(5/10)
HincII 29 GTCAAC GT[CT]^[GA]AC
HindII 29 GTCAAC GT[CT]^[GA]AC
HpaII 198 CCGG C^CGG
Ksp632I 202 GAAGAG CTCTTC(1/4)
MslI 933 CACTTTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 732 CAGCTG C[AC]G^C[GT]G
MspI 198 CCGG C^CGG
NciI 198 CCGGG CC^[GC]GG
NcoI 134 CCATGG C^CATGG
PpuMI 432 AGGTCCT [GA]G^G[AT]CC[CT]
PshAI 878 GACAGTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 118 CTGCAG CTGCA^G
PvuII 732 CAGCTG CAG^CTG
SacI 193 GAGCTC GAGCT^C
SfaNI 153 GCATC GCATC(5/9)
SfcI 118 CTGCAG C^T[GA][CT]AG
StuI 483 AGGCCT AGG^CCT
Tsp45I 566 GTGAC ^GT[GC]AC
TspRI 827 CACTG CA[GC]TG[ACGT][ACGT]^
XcmI 272 CCAAGGATATGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 315 GAACTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 24 enzymes) #
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AspHI 193 GAGCTC G[AT]GC[AT]^C
532 GTGCTC
AvaII 433 GGTCC G^G[AT]CC
504 GGACC
BanII 193 GAGCTC G[GA]GC[CT]^C
627 GGGCTC
BsiHKAI 193 GAGCTC G[AT]GC[AT]^C
532 GTGCTC
BsrDI 1001 CATTGC GCAATG(2/0)
258 GCAATG
BstF5I 18 CATCC GGATG(2/0)
1015 CATCC
DpnII 592 GATC ^GATC
790 GATC
EcoNI 748 CCTGAGCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
840 CCTCGACAAGG
EcoT14I 134 CCATGG C^C[AT][AT]GG
272 CCAAGG
FokI 18 CATCC GGATG(9/13)
1015 CATCC
HaeIII 421 GGCC GG^CC
484 GGCC
MaeIII 566 GTGAC ^GT[ACGT]AC
929 GTAAC
MboI 592 GATC ^GATC
790 GATC
NdeII 592 GATC ^GATC
790 GATC
NlaIV 504 GGACCC GG[ACGT]^[ACGT]CC
779 GGAACC
NspI 71 ACATGC [GA]CATG^[CT]
126 ACATGT
PflMI 441 CCAGTACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
551 CCAGCGTCTGG
PleI 607 GACTC GAGTC(4/5)
909 GACTC
Sau3AI 592 GATC ^GATC
790 GATC
SmlI 609 CTCAAG C^T[CT][GA]AG
630 CTCAAG
StyI 134 CCATGG C^C[AT][AT]GG
272 CCAAGG
TaqI 323 TCGA T^CGA
842 TCGA
TthHB8I 323 TCGA T^CGA
842 TCGA
Van91I 441 CCAGTACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
551 CCAGCGTCTGG
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# 3 OR MORE CUT ENZYMES ( 34 enzymes) #
#########################################
AfaI AluI AlwNI BbvI BmyI
BsaJI BseRI BsiYI BslI BsmAI
Bsp1286I BsrI BssKI BstNI Cac8I
Cfr13I DdeI EcoRII Fnu4HI HinfI
HphI ItaI MboII MnlI MseI
MvaI MwoI NlaIII RsaI Sau96I
ScrFI TfiI Tru9I TseI