Restriction map for
pFN21AE0697
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# NO CUT ENZYMES ( 90 enzymes) #
##################################
AatII AccI AccIII AclI AflII
AgeI Alw44I Aor51HI ApaLI AscI
AseI AsnI AspI AvrII BbrPI
BfrI BlnI BsaAI BsaWI BseAI
BsiEI BsiWI BsmBI Bsp1407I BspEI
BspHI BspLU11I BsrDI BsrGI BssSI
Bst1107I BstEII BstPI BstZ17I CpoI
DraI DraIII DsaI EagI EclXI
Eco47III Eco52I EcoNI EcoO65I FseI
HincII HindII HindIII HpaI KspI
MfeI MluI MroI MunI NcoI
NdeI NheI NotI NruI PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
PvuII RcaI RsrII SacI SacII
SalI SbfI ScaI SexAI SgrAI
SmlI SnaBI SpeI Sse8387I SspBI
SspI StuI SwaI Tth111I XhoI
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# ONE CUT ENZYMES ( 51 enzymes) #
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Acc65I 1543 GGTACC G^GTACC
AcsI 449 GAATTC [GA]^AATT[CT]
AcyI 965 GGCGCC G[GA]^CG[CT]C
AflIII 1472 ACGTGT A^C[GA][CT]GT
ApoI 449 GAATTC [GA]^AATT[CT]
Asp700I 457 GAAAAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1543 GGTACC G^GTACC
AspHI 1531 GTGCTC G[AT]GC[AT]^C
AvaI 1739 CCCGGG C^[CT]CG[GA]G
AviII 1568 TGCGCA TGC^GCA
BamHI 351 GGATCC G^GATCC
BbeI 965 GGCGCC GGCGC^C
BbvCI 1733 CCTCAGC CCTCAGC(-5/-2)
BcgI 1148 GCATTCTCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1680 GGATAC GTATCC(6/5)
BsaBI 1425 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 965 GGCGCC G[GA]^CG[CT]C
BsiHKAI 1531 GTGCTC G[AT]GC[AT]^C
BsoBI 1739 CCCGGG C^[CT]CG[GA]G
BspDI 631 ATCGAT AT^CGAT
BsrBI 2428 CCGCTC CCGCTC(-3/-3)
BssHII 40 GCGCGC G^CGCGC
BstBI 833 TTCGAA TT^CGAA
BstXI 2123 CCATCCTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
ClaI 631 ATCGAT AT^CGAT
DrdI 1624 GACTTCGGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 449 GAATTC G^AATTC
EcoRV 958 GATATC GAT^ATC
EcoT22I 1001 ATGCAT ATGCA^T
FspI 1568 TGCGCA TGC^GCA
Hin1I 965 GGCGCC G[GA]^CG[CT]C
KasI 965 GGCGCC G^GCGCC
KpnI 1543 GGTACC GGTAC^C
MamI 1425 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 2364 GCCGGC GCC^GGC
NarI 965 GGCGCC GG^CGCC
NgoMIV 2364 GCCGGC G^CCGGC
NsiI 1001 ATGCAT ATGCA^T
NspI 2300 GCATGC [GA]CATG^[CT]
NspV 833 TTCGAA TT^CGAA
SfiI 2367 GGCCTCCCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 965 GGCGCC GGC^GCC
SfuI 833 TTCGAA TT^CGAA
SmaI 1739 CCCGGG CCC^GGG
SphI 2300 GCATGC GCATG^C
Tsp509I 450 AATT ^AATT
XbaI 1254 TCTAGA T^CTAGA
XcmI 1643 CCATCCTGATCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 1739 CCCGGG C^CCGGG
XmaI 1739 CCCGGG C^CCGGG
XmnI 457 GAAAAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AhdI 1245 GACCAACGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2093 GACTCCTGGTC
AspEI 1245 GACCAACGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2093 GACTCCTGGTC
BalI 197 TGGCCA TGG^CCA
2525 TGGCCA
BclI 1424 TGATCA T^GATCA
1649 TGATCA
BglII 1370 AGATCT A^GATCT
1430 AGATCT
BlpI 1171 GCTCAGC GC^T[ACGT]AGC
2202 GCTCAGC
Bpu1102I 1171 GCTCAGC GC^T[ACGT]AGC
2202 GCTCAGC
BsaI 178 GAGACC GGTCTC(1/5)
1243 GAGACC
BsmI 1003 GCATTC GAATGC(1/-1)
1148 GCATTC
BspMI 170 ACCTGC ACCTGC(4/8)
1919 ACCTGC
BstAPI 519 GCAGCCGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
792 GCAGGCACTGC
CelII 1171 GCTCAGC GC^T[ACGT]AGC
2202 GCTCAGC
Eam1105I 1245 GACCAACGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2093 GACTCCTGGTC
EcoT14I 149 CCAAGG C^C[AT][AT]GG
2477 CCAAGG
FbaI 1424 TGATCA T^GATCA
1649 TGATCA
HaeII 839 GGCGCT [GA]GCGC^[CT]
965 GGCGCC
HgaI 1608 GCGTC GACGC(5/10)
2417 GCGTC
MluNI 197 TGGCCA TGG^CCA
2525 TGGCCA
MscI 197 TGGCCA TGG^CCA
2525 TGGCCA
MseI 281 TTAA T^TAA
895 TTAA
PflMI 1014 CCAGGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1755 CCACCCACTGG
SapI 399 GCTCTTC GCTCTTC(1/4)
936 GCTCTTC
StyI 149 CCAAGG C^C[AT][AT]GG
2477 CCAAGG
TfiI 462 GATTC G^A[AT]TC
472 GATTC
Tru9I 281 TTAA T^TAA
895 TTAA
Van91I 1014 CCAGGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1755 CCACCCACTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 91 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI AocI ApaI AvaII BanI
BanII BbsI BbvI BcnI BfaI
BglI BmyI BpmI BpuAI BsaJI
BseRI BsgI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrFI BsrI BssKI
BstF5I BstNI BstUI BstYI Bsu36I
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII EaeI EarI Eco81I
EcoO109I EcoRII FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PleI PpuMI PstI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
TaqI TseI Tsp45I TspRI TthHB8I
XhoII