##################################
# NO CUT ENZYMES (  236 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcyI        AfaI        AflII       
AflIII      AgeI        AhdI        Alw44I      AlwI        
AlwNI       AocI        Aor51HI     ApaI        ApaLI       
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspHI       AspI        AvaI        AvaII       
AviII       AvrII       BalI        BamHI       BanI        
BanII       BbeI        BbrPI       BbsI        BbvCI       
BbvI        BcgI        BciVI       BclI        BcnI        
BfaI        BfrI        BglI        BglII       BlnI        
BlpI        BmyI        Bpu1102I    BpuAI       BsaAI       
BsaBI       BsaHI       BsaI        BsaJI       BsaWI       
BseAI       BseRI       BsgI        BsiEI       BsiHKAI     
BsiWI       BsiYI       BslI        BsmBI       BsmFI       
BsmI        BsoBI       Bsp1286I    Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BspMI       BsrBI       
BsrDI       BsrFI       BsrGI       BssHII      BssKI       
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstF5I      BstNI       BstPI       BstUI       BstXI       
BstYI       BstZ17I     Bsu36I      Cac8I       CelII       
CfoI        Cfr10I      Cfr13I      ClaI        CpoI        
DdeI        DraI        DraII       DraIII      DrdI        
DsaI        EaeI        EagI        Eam1105I    EarI        
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO109I    EcoO65I     EcoRI       EcoRII      EcoRV       
EcoT14I     EcoT22I     FauI        FbaI        Fnu4HI      
FokI        FseI        FspI        HaeII       HaeIII      
HapII       HgaI        HhaI        Hin1I       HinP1I      
HincII      HindII      HindIII     HpaI        HpaII       
HphI        ItaI        KasI        KpnI        Ksp632I     
KspI        MaeI        MaeII       MaeIII      MamI        
MboII       MfeI        MflI        MluI        MluNI       
MroI        MscI        MslI        MspA1I      MspI        
MunI        MvaI        MvnI        MwoI        NaeI        
NarI        NciI        NcoI        NdeI        NgoMIV      
NheI        NlaIV       NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PleI        PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       Psp1406I    PstI        
PvuI        PvuII       RcaI        RsaI        RsrII       
SacI        SacII       SalI        SapI        Sau96I      
SbfI        ScaI        ScrFI       SexAI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SmlI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        StuI        StyI        SwaI        TseI        
Tsp45I      TspRI       Tth111I     Van91I      XbaI        
XcmI        XhoI        XhoII       XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   20 enzymes) #
###################################

AcsI           94  AAATTC               [GA]^AATT[CT]
AluI           54  AGCT                 AG^CT
ApoI           94  AAATTC               [GA]^AATT[CT]
BpmI           50  CTGGAG               CTGGAG(16/14)
BsmAI          72  GAGAC                GTCTC(1/5)
BsrI           49  ACTGG                ACTGG(1/-1)
DpnII         131  GATC                 ^GATC
HinfI          58  GATTC                G^A[ACGT]TC
MboI          131  GATC                 ^GATC
MnlI          122  GAGG                 CCTC(7/6)
MseI           22  TTAA                 T^TAA
NdeII         131  GATC                 ^GATC
NspI           63  ACATGC               [GA]CATG^[CT]
Sau3AI        131  GATC                 ^GATC
SfaNI         108  GCATC                GCATC(5/9)
SfcI          126  CTATAG               C^T[GA][CT]AG
TaqI          120  TCGA                 T^CGA
TfiI           58  GATTC                G^A[AT]TC
Tru9I          22  TTAA                 T^TAA
TthHB8I       120  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (    1 enzymes) #
###################################

Tsp509I        95  AATT                 ^AATT
              136  AATT                


#########################################
# 3 OR MORE CUT ENZYMES (    1 enzymes) #
#########################################

NlaIII