Restriction map for
pFN21AE0899
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# NO CUT ENZYMES ( 67 enzymes) #
##################################
Acc65I AclI AflII AgeI Aor51HI
AscI Asp718I AspI AvrII BbeI
BcgI BclI BfrI BlnI BsaBI
BsiWI Bsp1407I BspDI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstZ17I ClaI DraI Eco47III
EcoO65I FbaI FseI HindIII HpaI
KasI KpnI KspI MamI MfeI
MluI MunI NarI NdeI NheI
NotI NruI NspV PacI PflFI
PinAI PmeI PshAI Psp1406I PvuI
SacII SbfI SexAI SfiI SfoI
SfuI SpeI Sse8387I SspBI SwaI
Tth111I XbaI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AccI 621 GTCGAC GT^[AC][GT]AC
AccIII 1776 TCCGGA T^CCGGA
AcsI 123 GAATTC [GA]^AATT[CT]
AhdI 2125 GACTCCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 1677 CCTCAGG CC^T[ACGT]AGG
ApoI 123 GAATTC [GA]^AATT[CT]
AseI 1984 ATTAAT AT^TAAT
AsnI 1984 ATTAAT AT^TAAT
AspEI 2125 GACTCCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 2051 TGCGCA TGC^GCA
BamHI 3037 GGATCC G^GATCC
BanI 2914 GGCACC G^G[CT][GA]CC
BbvCI 2860 GCTGAGG CCTCAGC(-5/-2)
BciVI 3267 GGATAC GTATCC(6/5)
BlpI 3157 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 3157 GCTAAGC GC^T[ACGT]AGC
BseAI 1776 TCCGGA T^CCGGA
BsmBI 3397 GAGACG CGTCTC(1/5)
BsmI 2852 GCATTC GAATGC(1/-1)
BspEI 1776 TCCGGA T^CCGGA
BspLU11I 651 ACATGT A^CATGT
BstXI 509 CCAACAGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1677 CCTCAGG CC^T[ACGT]AGG
CelII 3157 GCTAAGC GC^T[ACGT]AGC
CpoI 1727 CGGTCCG CG^G[AT]CCG
DrdI 2407 GACAAAGGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 2125 GACTCCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1677 CCTCAGG CC^T[ACGT]AGG
EcoNI 2651 CCTTTTCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 123 GAATTC G^AATTC
EcoRV 429 GATATC GAT^ATC
EcoT22I 251 ATGCAT ATGCA^T
FspI 2051 TGCGCA TGC^GCA
MroI 1776 TCCGGA T^CCGGA
MseI 1985 TTAA T^TAA
NsiI 251 ATGCAT ATGCA^T
PaeR7I 2786 CTCGAG C^TCGAG
PpuMI 3654 AGGTCCC [GA]G^G[AT]CC[CT]
PshBI 1984 ATTAAT AT^TAAT
RsrII 1727 CGGTCCG CG^G[AT]CCG
SalI 621 GTCGAC G^TCGAC
SapI 1515 GAAGAGC GCTCTTC(1/4)
ScaI 2546 AGTACT AGT^ACT
SgrAI 2146 CACCGGCG C[GA]^CCGG[CT]G
SmaI 3033 CCCGGG CCC^GGG
SnaBI 86 TACGTA TAC^GTA
SspI 2752 AATATT AAT^ATT
StuI 2096 AGGCCT AGG^CCT
Tru9I 1985 TTAA T^TAA
Tsp509I 124 AATT ^AATT
XhoI 2786 CTCGAG C^TCGAG
XmaCI 3033 CCCGGG C^CCGGG
XmaI 3033 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AatII 580 GACGTC GACGT^C
3399 GACGTC
AccII 976 CGCG CG^CG
1732 CGCG
AflIII 651 ACATGT A^C[GA][CT]GT
1449 ACACGT
ApaI 1201 GGGCCC GGGCC^C
1863 GGGCCC
BalI 2081 TGGCCA TGG^CCA
3012 TGGCCA
BbsI 577 GAAGAC GAAGAC(2/6)
2346 GAAGAC
BglI 1645 GCCGGGTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3335 GCCACGATGGC
BglII 3419 AGATCT A^GATCT
3584 AGATCT
BpuAI 577 GAAGAC GAAGAC(2/6)
2346 GAAGAC
BsaWI 1587 ACCGGA [AT]^CCGG[AT]
1776 TCCGGA
BspHI 2495 TCATGA T^CATGA
2948 TCATGA
BsrBI 1708 CCGCTC CCGCTC(-3/-3)
1936 CCGCTC
BstUI 976 CGCG CG^CG
1732 CGCG
DraIII 2284 CACGGCGTG CAC[ACGT][ACGT][ACGT]^GTG
3099 CACCCAGTG
HaeII 1555 AGCGCC [GA]GCGC^[CT]
1856 GGCGCT
MluNI 2081 TGGCCA TGG^CCA
3012 TGGCCA
MscI 2081 TGGCCA TGG^CCA
3012 TGGCCA
MvnI 976 CGCG CG^CG
1732 CGCG
NaeI 1853 GCCGGC GCC^GGC
2354 GCCGGC
NgoMIV 1853 GCCGGC G^CCGGC
2354 GCCGGC
RcaI 2495 TCATGA T^CATGA
2948 TCATGA
SphI 141 GCATGC GCATG^C
2000 GCATGC
#########################################
# 3 OR MORE CUT ENZYMES ( 116 enzymes) #
#########################################
AciI AcyI AfaI AluI Alw44I
AlwI AlwNI ApaLI Asp700I AspHI
AvaI AvaII BanII BbrPI BbvI
BcnI BfaI BmyI BpmI BsaAI
BsaHI BsaI BsaJI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrDI
BsrFI BsrI BssKI BstF5I BstNI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EagI EarI EclXI Eco52I EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MslI MspA1I MspI MvaI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PflMI PleI PmaCI PmlI
PstI PvuII RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TaqI TfiI TseI Tsp45I
TspRI TthHB8I Van91I XcmI XhoII
XmnI