##################################
# NO CUT ENZYMES (  207 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcsI        AcyI        AflII       
AflIII      AgeI        AhdI        AluI        Alw44I      
AlwI        AlwNI       AocI        Aor51HI     ApaI        
ApaLI       ApoI        AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AspHI       AspI        
AvaII       AviII       AvrII       BamHI       BanII       
BbeI        BbrPI       BbsI        BbvCI       BcgI        
BciVI       BclI        BcnI        BfaI        BfrI        
BglI        BglII       BlnI        BlpI        BpmI        
Bpu1102I    BpuAI       BsaAI       BsaBI       BsaHI       
BsaWI       BseAI       BsgI        BsiEI       BsiHKAI     
BsiWI       BsmBI       BsmFI       BsmI        Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BspMI       
BsrBI       BsrFI       BsrGI       BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstF5I      
BstPI       BstUI       BstYI       BstZ17I     Bsu36I      
CelII       CfoI        Cfr10I      ClaI        CpoI        
DdeI        DpnII       DraI        DraII       DraIII      
DrdI        DsaI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoO109I    
EcoO65I     EcoRI       EcoRV       EcoT22I     FauI        
FbaI        FokI        FseI        FspI        HaeII       
HapII       HgaI        HhaI        Hin1I       HinP1I      
HindIII     HinfI       HpaI        HpaII       HphI        
KasI        KpnI        KspI        MaeI        MaeII       
MamI        MboI        MfeI        MflI        MluI        
MroI        MseI        MspA1I      MspI        MunI        
MvnI        NaeI        NarI        NciI        NcoI        
NdeI        NdeII       NgoMIV      NheI        NlaIII      
NotI        NruI        NsiI        NspI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PleI        PmaCI       PmeI        PmlI        PpuMI       
PshAI       PshBI       Psp1406I    PvuI        PvuII       
RcaI        RsrII       SacI        SacII       SalI        
SapI        Sau3AI      SbfI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SmlI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        StuI        SwaI        TaqI        TfiI        
Tru9I       Tsp45I      Tsp509I     Tth111I     TthHB8I     
Van91I      XbaI        XhoI        XhoII       XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   35 enzymes) #
###################################

AfaI          119  GTAC                 GT^AC
AvaI           25  CTCGGG               C^[CT]CG[GA]G
BalI           17  TGGCCA               TGG^CCA
BanI          178  GGTGCC               G^G[CT][GA]CC
BmyI          179  GTGCCC               G[AGT]GC[ACT]^C
BsaI          210  GGTCTC               GGTCTC(1/5)
BsaJI         164  CCAAGG               C^C[ACGT][ACGT]GG
BseRI         108  CTCCTC               GAGGAG(10/8)
BsoBI          25  CTCGGG               C^[CT]CG[GA]G
Bsp1286I      179  GTGCCC               G[AGT]GC[ACT]^C
BsrDI          10  GCAATG               GCAATG(2/0)
BssKI          20  CCAGG                ^CC[ACGT]GG
BstNI          20  CCAGG                CC^[AT]GG
BstXI         161  CCACCAAGGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cac8I          45  GCTGGC               GC[ACGT]^[ACGT]GC
Cfr13I        138  GGCCC                G^G[ACGT]CC
EaeI           17  TGGCCA               [CT]^GGCC[GA]
EcoRII         20  CCAGG                ^CC[AT]GG
EcoT14I       164  CCAAGG               C^C[AT][AT]GG
HincII        189  GTCAAC               GT[CT]^[GA]AC
HindII        189  GTCAAC               GT[CT]^[GA]AC
MaeIII        104  GTAAC                ^GT[ACGT]AC
MluNI          17  TGGCCA               TGG^CCA
MnlI          110  CCTC                 CCTC(7/6)
MscI           17  TGGCCA               TGG^CCA
MslI          162  CACCAAGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvaI           20  CCAGG                CC^[AT]GG
PstI          184  CTGCAG               CTGCA^G
RsaI          119  GTAC                 GT^AC
Sau96I        138  GGCCC                G^G[ACGT]CC
ScaI          118  AGTACT               AGT^ACT
ScrFI          20  CCAGG                CC^[ACGT]GG
SfaNI         228  GCATC                GCATC(5/9)
SfcI          184  CTGCAG               C^T[GA][CT]AG
StyI          164  CCAAGG               C^C[AT][AT]GG


###################################
# TWO CUT ENZYMES (    9 enzymes) #
###################################

BsiYI          20  CCAGGCTCGGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              125  CCAACTCTGGG         
BslI           20  CCAGGCTCGGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              125  CCAACTCTGGG         
BsmAI         200  GTCTC                GTCTC(1/5)
              211  GTCTC               
EarI          202  CTCTTC               CTCTTC(1/4)
              221  CTCTTC              
HaeIII         18  GGCC                 GG^CC
              138  GGCC                
Ksp632I       202  CTCTTC               CTCTTC(1/4)
              221  CTCTTC              
MboII         203  TCTTC                GAAGA(8/7)
              222  TCTTC               
NlaIV         138  GGCCCC               GG[ACGT]^[ACGT]CC
              178  GGTGCC              
TspRI          50  CACTG                CA[GC]TG[ACGT][ACGT]^
               78  CAGTG               


#########################################
# 3 OR MORE CUT ENZYMES (    7 enzymes) #
#########################################

BbvI        BsrI        Fnu4HI      ItaI        MwoI        
TseI        XcmI