Restriction map for
pFN21AE1501
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# NO CUT ENZYMES ( 153 enzymes) #
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Acc65I AccI AclI AcsI AflII
AflIII AgeI AhdI Alw44I AlwNI
AocI Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AspI AviII AvrII
BalI BamHI BbeI BbrPI BbsI
BbvCI BcgI BciVI BclI BfaI
BfrI BglII BlnI BlpI Bpu1102I
BpuAI BsaAI BsaBI BsaI BsiEI
BsiHKAI BsiWI BsmI Bsp1407I BspDI
BspHI BspLU11I BspMI BsrBI BsrFI
BsrGI BssSI Bst1107I BstAPI BstBI
BstEII BstF5I BstPI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DraI
DrdI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FbaI FokI
FseI FspI HaeII HgaI HincII
HindII HindIII HpaI KasI KpnI
Ksp632I KspI MaeI MamI MfeI
MluI MluNI MscI MseI MunI
NaeI NarI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SapI ScaI SexAI SfoI
SfuI SgrAI SnaBI SpeI SphI
SspBI SspI SwaI TaqI TfiI
Tru9I Tsp509I Tth111I TthHB8I Van91I
XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 30 enzymes) #
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AccIII 783 TCCGGA T^CCGGA
AfaI 138 GTAC GT^AC
AlwI 38 GATCC GGATC(4/5)
BsaWI 783 TCCGGA [AT]^CCGG[AT]
BseAI 783 TCCGGA T^CCGGA
BsmBI 714 CGTCTC CGTCTC(1/5)
BspEI 783 TCCGGA T^CCGGA
BsrDI 249 CATTGC GCAATG(2/0)
BssHII 315 GCGCGC G^CGCGC
BstXI 255 CCACCTCAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 37 AGATCC [GA]^GATC[CT]
EaeI 800 TGGCCG [CT]^GGCC[GA]
FauI 200 GCGGG CCCGC(4/6)
HinfI 468 GAGTC G^A[ACGT]TC
MboII 680 GAAGA GAAGA(8/7)
MflI 37 AGATCC [GA]^GATC[CT]
MroI 783 TCCGGA T^CCGGA
NcoI 305 CCATGG C^CATGG
NdeI 666 CATATG CA^TATG
NlaIII 306 CATG CATG^
PleI 468 GAGTC GAGTC(4/5)
PvuII 555 CAGCTG CAG^CTG
RsaI 138 GTAC GT^AC
SbfI 771 CCTGCAGG CCTGCA^GG
SfaNI 380 GCATC GCATC(5/9)
SfiI 768 GGCCCTGCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI 60 CTTGAG C^T[CT][GA]AG
Sse8387I 771 CCTGCAGG CCTGCA^GG
StuI 194 AGGCCT AGG^CCT
XhoII 37 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 23 enzymes) #
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AatII 55 GACGTC GACGT^C
787 GACGTC
AcyI 55 GACGTC G[GA]^CG[CT]C
787 GACGTC
AvaI 435 CCCGGG C^[CT]CG[GA]G
498 CCCGGG
BanI 241 GGCACC G^G[CT][GA]CC
460 GGCACC
BglI 233 GCCCTCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
769 GCCCTGCAGGC
BsaHI 55 GACGTC G[GA]^CG[CT]C
787 GACGTC
BsoBI 435 CCCGGG C^[CT]CG[GA]G
498 CCCGGG
DdeI 268 CTAAG C^T[ACGT]AG
618 CTCAG
DpnII 38 GATC ^GATC
711 GATC
DraIII 216 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG
477 CACCCTGTG
EcoT14I 305 CCATGG C^C[AT][AT]GG
688 CCAAGG
Hin1I 55 GACGTC G[GA]^CG[CT]C
787 GACGTC
MaeII 56 ACGT A^CGT
788 ACGT
MboI 38 GATC ^GATC
711 GATC
MspA1I 555 CAGCTG C[AC]G^C[GT]G
724 CAGCGG
NdeII 38 GATC ^GATC
711 GATC
PpuMI 502 GGGACCT [GA]G^G[AT]CC[CT]
520 GGGACCC
Sau3AI 38 GATC ^GATC
711 GATC
SmaI 435 CCCGGG CCC^GGG
498 CCCGGG
StyI 305 CCATGG C^C[AT][AT]GG
688 CCAAGG
XcmI 515 CCAATGGGACCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
794 CCACGGTGGCCGTGG
XmaCI 435 CCCGGG C^CCGGG
498 CCCGGG
XmaI 435 CCCGGG C^CCGGG
498 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
#########################################
AccII AciI AluI AvaII BanII
BbvI BcnI BmyI BpmI BsaJI
BseRI BsgI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrI BssKI BstNI
BstUI Cac8I CfoI Cfr13I DraII
DsaI EcoO109I EcoRII Fnu4HI HaeIII
HapII HhaI HinP1I HpaII HphI
ItaI MaeIII MnlI MslI MspI
MvaI MvnI MwoI NciI NlaIV
PstI Sau96I ScrFI SfcI TseI
Tsp45I TspRI