Restriction map for
pFN21AE1017
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# NO CUT ENZYMES ( 136 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AflII AflIII AgeI AlwNI
ApaI AscI AseI AsnI Asp700I
Asp718I AspI AvaI AviII AvrII
BamHI BanI BbeI BbrPI BcgI
BciVI BclI BfrI BglII BlnI
BlpI Bpu1102I BsaAI BsaBI BsaI
BsaWI BseAI BsiEI BsiWI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrDI BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstUI
BstXI BstZ17I CelII ClaI CpoI
DraI DraIII DrdI EagI EclXI
Eco52I EcoO65I EcoRI EcoT14I EcoT22I
FbaI FseI FspI HincII HindII
HpaI KasI KpnI KspI MaeII
MamI MfeI MluI MroI MspA1I
MunI MvnI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI PvuII RcaI
RsrII SacI SacII SalI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI Sse8387I
SspBI SspI StuI StyI SwaI
Tth111I XbaI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AcyI 317 GGCGTC G[GA]^CG[CT]C
AhdI 673 GACCAGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 800 GTGCAC G^TGCAC
AlwI 1038 GGATC GGATC(4/5)
AocI 874 CCTAAGG CC^T[ACGT]AGG
Aor51HI 1253 AGCGCT AGC^GCT
ApaLI 800 GTGCAC G^TGCAC
AspEI 673 GACCAGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 362 TGGCCA TGG^CCA
BbsI 1290 GAAGAC GAAGAC(2/6)
BbvCI 703 CCTCAGC CCTCAGC(-5/-2)
BglI 984 GCCTTCAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 1290 GAAGAC GAAGAC(2/6)
BsaHI 317 GGCGTC G[GA]^CG[CT]C
BsmI 509 GCATTC GAATGC(1/-1)
BsrFI 1196 ACCGGC [GA]^CCGG[CT]
BstAPI 1200 GCACATCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 1038 GGATCT [GA]^GATC[CT]
Bsu36I 874 CCTAAGG CC^T[ACGT]AGG
Cfr10I 1196 ACCGGC [GA]^CCGG[CT]
Eam1105I 673 GACCAGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1253 AGCGCT AGC^GCT
Eco81I 874 CCTAAGG CC^T[ACGT]AGG
EcoRV 885 GATATC GAT^ATC
FauI 31 CCCGC CCCGC(4/6)
HaeII 1253 AGCGCT [GA]GCGC^[CT]
HgaI 318 GCGTC GACGC(5/10)
Hin1I 317 GGCGTC G[GA]^CG[CT]C
HindIII 781 AAGCTT A^AGCTT
HphI 793 GGTGA GGTGA(8/7)
MaeIII 1193 GTGAC ^GT[ACGT]AC
MflI 1038 GGATCT [GA]^GATC[CT]
MluNI 362 TGGCCA TGG^CCA
MscI 362 TGGCCA TGG^CCA
MseI 854 TTAA T^TAA
MslI 1045 CATGGGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NspI 1311 GCATGC [GA]CATG^[CT]
PflMI 1000 CCAACATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 1133 CTGCAG CTGCA^G
SmlI 808 CTTGAG C^T[CT][GA]AG
SphI 1311 GCATGC GCATG^C
TfiI 106 GAATC G^A[AT]TC
Tru9I 854 TTAA T^TAA
Tsp45I 1193 GTGAC ^GT[GC]AC
Van91I 1000 CCAACATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1092 CCAGGCCGTGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 1038 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AfaI 28 GTAC GT^AC
538 GTAC
BcnI 168 CCGGG CC^[GC]GG
1368 CCGGG
BsgI 827 CTGCAC GTGCAG(16/14)
160 GTGCAG
BsmAI 251 GTCTC GTCTC(1/5)
320 GTCTC
BsmBI 250 CGTCTC CGTCTC(1/5)
319 CGTCTC
BspMI 43 ACCTGC ACCTGC(4/8)
384 GCAGGT
CfoI 244 GCGC GCG^C
1254 GCGC
DraII 1074 AGGACCT [GA]G^G[ACGT]CC[CT]
1370 GGGACCT
DsaI 58 CCACGG C^C[GA][CT]GG
450 CCGTGG
EaeI 362 TGGCCA [CT]^GGCC[GA]
1329 TGGCCG
EcoO109I 1074 AGGACCT [GA]G^G[ACGT]CC[CT]
1370 GGGACCT
HhaI 244 GCGC GCG^C
1254 GCGC
HinP1I 244 GCGC G^CGC
1254 GCGC
NciI 168 CCGGG CC^[GC]GG
1368 CCGGG
PleI 699 GACTC GAGTC(4/5)
1343 GAGTC
PpuMI 1074 AGGACCT [GA]G^G[AT]CC[CT]
1370 GGGACCT
RsaI 28 GTAC GT^AC
538 GTAC
SapI 340 GCTCTTC GCTCTTC(1/4)
515 GCTCTTC
SfcI 494 CTACAG C^T[GA][CT]AG
1133 CTGCAG
#########################################
# 3 OR MORE CUT ENZYMES ( 56 enzymes) #
#########################################
AciI AcsI AluI ApoI AspHI
AvaII BanII BbvI BfaI BmyI
BpmI BsaJI BseRI BsiHKAI BsiYI
BslI BsmFI Bsp1286I BsrI BssKI
BstF5I BstNI Cac8I Cfr13I DdeI
DpnII EarI EcoNI EcoRII Fnu4HI
FokI HaeIII HapII HinfI HpaII
ItaI Ksp632I MaeI MboI MboII
MnlI MspI MvaI MwoI NdeII
NlaIII NlaIV Sau3AI Sau96I ScrFI
SfaNI TaqI TseI Tsp509I TspRI
TthHB8I