Restriction map for
pFN21AE1634
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# NO CUT ENZYMES ( 184 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AcyI AfaI AflII AflIII
AgeI AhdI Alw44I AlwNI AocI
Aor51HI ApaLI ApoI AseI AsnI
Asp700I Asp718I AspEI AspI AvaI
AvaII AviII AvrII BalI BanI
BbeI BbrPI BbsI BbvCI BcgI
BciVI BclI BcnI BfaI BfrI
BglI BglII BlnI BlpI BpmI
Bpu1102I BpuAI BsaAI BsaBI BsaHI
BsaI BseAI BseRI BsiEI BsiWI
BsmBI BsmFI BsmI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrDI BsrGI BsrI BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstXI
BstZ17I Bsu36I CelII ClaI CpoI
DdeI DraI DraIII DrdI DsaI
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoT22I FbaI FseI FspI HaeII
HgaI Hin1I HincII HindII HindIII
HinfI HpaI KasI KpnI Ksp632I
KspI MaeI MaeII MaeIII MamI
MboII MfeI MluI MluNI MroI
MscI MseI MslI MunI NarI
NciI NcoI NdeI NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PinAI PleI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI PvuII RcaI
RsaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfcI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI TfiI
Tru9I Tsp45I TspRI Tth111I XbaI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 35 enzymes) #
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AccII 133 CGCG CG^CG
ApaI 50 GGGCCC GGGCC^C
AscI 131 GGCGCGCC GG^CGCGCC
AspHI 124 GTGCTC G[AT]GC[AT]^C
BamHI 149 GGATCC G^GATCC
BanII 50 GGGCCC G[GA]GC[CT]^C
BsaWI 146 ACCGGA [AT]^CCGG[AT]
BsgI 247 GTGCAG GTGCAG(16/14)
BsiHKAI 124 GTGCTC G[AT]GC[AT]^C
BsrBI 162 GAGCGG CCGCTC(-3/-3)
BsrFI 31 GCCGGC [GA]^CCGG[CT]
BssHII 132 GCGCGC G^CGCGC
BstUI 133 CGCG CG^CG
BstYI 149 GGATCC [GA]^GATC[CT]
Cfr10I 31 GCCGGC [GA]^CCGG[CT]
DpnII 150 GATC ^GATC
EaeI 29 TGGCCG [CT]^GGCC[GA]
EcoRV 426 GATATC GAT^ATC
HphI 159 GGTGA GGTGA(8/7)
MboI 150 GATC ^GATC
MflI 149 GGATCC [GA]^GATC[CT]
MspA1I 56 CCGCTG C[AC]G^C[GT]G
MvnI 133 CGCG CG^CG
MwoI 164 GCGGGAGCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NaeI 31 GCCGGC GCC^GGC
NdeII 150 GATC ^GATC
NgoMIV 31 GCCGGC G^CCGGC
PflMI 110 CCATTGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Sau3AI 150 GATC ^GATC
SfaNI 271 GATGC GCATC(5/9)
TaqI 276 TCGA T^CGA
Tsp509I 436 AATT ^AATT
TthHB8I 276 TCGA T^CGA
Van91I 110 CCATTGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 149 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AciI 56 CCGC CCGC(-3/-1)
164 GCGG
AlwI 150 GATCC GGATC(4/5)
149 GGATC
BmyI 50 GGGCCC G[AGT]GC[ACT]^C
124 GTGCTC
BsmAI 207 GAGAC GTCTC(1/5)
415 GTCTC
Bsp1286I 50 GGGCCC G[AGT]GC[ACT]^C
124 GTGCTC
BstF5I 374 CATCC GGATG(2/0)
270 GGATG
CfoI 132 GCGC GCG^C
134 GCGC
DraII 50 GGGCCCC [GA]G^G[ACGT]CC[CT]
324 AGGCCCT
EcoO109I 50 GGGCCCC [GA]G^G[ACGT]CC[CT]
324 AGGCCCT
EcoT14I 69 CCAAGG C^C[AT][AT]GG
321 CCAAGG
FauI 55 CCCGC CCCGC(4/6)
164 GCGGG
FokI 374 CATCC GGATG(9/13)
270 GGATG
HhaI 132 GCGC GCG^C
134 GCGC
HinP1I 132 GCGC G^CGC
134 GCGC
NlaIII 258 CATG CATG^
331 CATG
StyI 69 CCAAGG C^C[AT][AT]GG
321 CCAAGG
XcmI 102 CCACTACCCCATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
257 CCATGAAGCTCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 22 enzymes) #
#########################################
AluI BbvI BsaJI BsiYI BslI
BssKI BstNI Cac8I Cfr13I EcoRII
Fnu4HI HaeIII HapII HpaII ItaI
MnlI MspI MvaI NlaIV Sau96I
ScrFI TseI