Restriction map for
pFN21AE1732
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# NO CUT ENZYMES ( 154 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AfaI AflII
AflIII AhdI Alw44I Aor51HI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AviII AvrII BalI
BamHI BanI BbeI BbrPI BbsI
BbvCI BcgI BciVI BfaI BfrI
BglII BlnI BlpI BpmI Bpu1102I
BpuAI BsaAI BsaBI BsaHI BseAI
BsgI BsiWI BsmBI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrDI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstYI BstZ17I CelII ClaI CpoI
DraI DraIII EaeI EagI Eam1105I
EarI EclXI Eco47III Eco52I EcoO65I
EcoRI EcoRV EcoT14I EcoT22I FseI
FspI Hin1I HpaI KasI KpnI
Ksp632I KspI MaeI MamI MfeI
MflI MluI MluNI MroI MscI
MunI MvnI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuII RcaI RsaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI StyI
SwaI Tsp509I XbaI XcmI XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 36 enzymes) #
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AgeI 214 ACCGGT A^CCGGT
AlwNI 293 CAGGCACTG CAG[ACGT][ACGT][ACGT]^CTG
AspHI 571 GAGCAC G[AT]GC[AT]^C
AvaI 60 CTCGGG C^[CT]CG[GA]G
BclI 608 TGATCA T^GATCA
BsaI 637 GAGACC GGTCTC(1/5)
BsaWI 214 ACCGGT [AT]^CCGG[AT]
BsiEI 176 CGATCG CG[GA][CT]^CG
BsiHKAI 571 GAGCAC G[AT]GC[AT]^C
BsoBI 60 CTCGGG C^[CT]CG[GA]G
BsrBI 47 CCGCTC CCGCTC(-3/-3)
CfoI 378 GCGC GCG^C
DrdI 457 GACAAGCCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 434 CCACGG C^C[GA][CT]GG
EcoNI 417 CCTGAGGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 608 TGATCA T^GATCA
HaeII 377 GGCGCT [GA]GCGC^[CT]
HgaI 130 GACGC GACGC(5/10)
HhaI 378 GCGC GCG^C
HinP1I 378 GCGC G^CGC
HincII 466 GTCAAC GT[CT]^[GA]AC
HindII 466 GTCAAC GT[CT]^[GA]AC
HindIII 519 AAGCTT A^AGCTT
MaeII 191 ACGT A^CGT
MaeIII 218 GTGAC ^GT[ACGT]AC
MboII 9 GAAGA GAAGA(8/7)
MseI 272 TTAA T^TAA
MslI 628 CACCCGTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
PflMI 66 CCAGAGCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 214 ACCGGT A^CCGGT
PpuMI 356 GGGACCT [GA]G^G[AT]CC[CT]
PvuI 176 CGATCG CGAT^CG
SgrAI 213 CACCGGTG C[GA]^CCGG[CT]G
Tru9I 272 TTAA T^TAA
Tsp45I 218 GTGAC ^GT[GC]AC
Van91I 66 CCAGAGCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 16 enzymes) #
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AlwI 621 GATCC GGATC(4/5)
209 GGATC
AocI 235 CCTGAGG CC^T[ACGT]AGG
417 CCTGAGG
ApaI 55 GGGCCC GGGCC^C
142 GGGCCC
BanII 55 GGGCCC G[GA]GC[CT]^C
142 GGGCCC
BglI 28 GCCGGGACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
174 GCCGATCGGGC
BseRI 238 GAGGAG GAGGAG(10/8)
601 GAGGAG
BspMI 565 ACCTGC ACCTGC(4/8)
423 GCAGGT
BstF5I 546 CATCC GGATG(2/0)
666 GGATG
Bsu36I 235 CCTGAGG CC^T[ACGT]AGG
417 CCTGAGG
Eco81I 235 CCTGAGG CC^T[ACGT]AGG
417 CCTGAGG
FauI 354 GCGGG CCCGC(4/6)
710 GCGGG
FokI 546 CATCC GGATG(9/13)
666 GGATG
NlaIII 285 CATG CATG^
480 CATG
PstI 160 CTGCAG CTGCA^G
202 CTGCAG
SfaNI 681 GATGC GCATC(5/9)
545 GCATC
TspRI 297 CACTG CA[GC]TG[ACGT][ACGT]^
449 CAGTG
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# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
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AciI AluI AspI AvaII BbvI
BcnI BmyI BsaJI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrFI BsrI
BssKI BstNI Cac8I Cfr10I Cfr13I
DdeI DpnII DraII EcoO109I EcoRII
Fnu4HI HaeIII HapII HinfI HpaII
HphI ItaI MboI MnlI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIV PflFI PleI Sau3AI Sau96I
ScrFI SfcI TaqI TfiI TseI
Tth111I TthHB8I