Restriction map for
pFN21AE1685
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# NO CUT ENZYMES ( 160 enzymes) #
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AatII AccI AccIII AclI AcsI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwI ApaI ApaLI ApoI
AscI AseI AsnI Asp700I AspEI
AspHI AspI AviII AvrII BalI
BamHI BbeI BbrPI BbsI BciVI
BclI BfaI BfrI BglI BglII
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BsiHKAI BsiWI BsmBI BsmI Bsp1407I
BspEI BspHI BspLU11I BsrBI BsrDI
BsrGI BssHII Bst1107I BstBI BstEII
BstPI BstXI BstYI BstZ17I CelII
CpoI DpnII DraI DraIII DrdI
DsaI Eam1105I EarI EcoO65I EcoRI
EcoRV EcoT14I EcoT22I FbaI FseI
FspI Hin1I HincII HindII HindIII
HinfI HpaI KasI Ksp632I KspI
MaeI MamI MboI MboII MfeI
MflI MluI MluNI MroI MscI
MseI MunI NarI NcoI NdeI
NdeII NheI NotI NruI NsiI
NspV PacI PflFI PflMI PinAI
PleI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
RcaI RsrII SacI SacII SalI
SapI Sau3AI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI StyI SwaI TfiI
Tru9I Tsp509I Tth111I Van91I XbaI
XcmI XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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Acc65I 94 GGTACC G^GTACC
AfaI 95 GTAC GT^AC
AocI 69 CCTCAGG CC^T[ACGT]AGG
Aor51HI 308 AGCGCT AGC^GCT
Asp718I 94 GGTACC G^GTACC
AvaII 351 GGACC G^G[AT]CC
BanII 505 GGGCTC G[GA]GC[CT]^C
BcgI 423 CGAGACAGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsiEI 274 CGGCCG CG[GA][CT]^CG
BsmAI 424 GAGAC GTCTC(1/5)
BsmFI 553 GTCCC GGGAC(10/14)
BspDI 403 ATCGAT AT^CGAT
BsrFI 479 GCCGGC [GA]^CCGG[CT]
BsrI 258 CCAGT ACTGG(1/-1)
BssSI 235 CACGAG CACGAG(-5/-1)
BstAPI 177 GCAGGCTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 69 CCTCAGG CC^T[ACGT]AGG
Cfr10I 479 GCCGGC [GA]^CCGG[CT]
ClaI 403 ATCGAT AT^CGAT
EagI 274 CGGCCG C^GGCCG
EclXI 274 CGGCCG C^GGCCG
Eco47III 308 AGCGCT AGC^GCT
Eco52I 274 CGGCCG C^GGCCG
Eco81I 69 CCTCAGG CC^T[ACGT]AGG
FauI 491 CCCGC CCCGC(4/6)
HaeII 308 AGCGCT [GA]GCGC^[CT]
HgaI 3 GACGC GACGC(5/10)
HphI 319 TCACC GGTGA(8/7)
KpnI 94 GGTACC GGTAC^C
MaeII 551 ACGT A^CGT
MaeIII 89 GTCAC ^GT[ACGT]AC
MslI 567 CACCTGCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 428 CAGCTG C[AC]G^C[GT]G
NaeI 479 GCCGGC GCC^GGC
NgoMIV 479 GCCGGC G^CCGGC
NspI 43 ACATGC [GA]CATG^[CT]
PaeR7I 158 CTCGAG C^TCGAG
PvuII 428 CAGCTG CAG^CTG
RsaI 95 GTAC GT^AC
SfaNI 406 GATGC GCATC(5/9)
SfcI 188 CTGTAG C^T[GA][CT]AG
SmlI 158 CTCGAG C^T[CT][GA]AG
Tsp45I 89 GTCAC ^GT[GC]AC
XhoI 158 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AccII 421 CGCG CG^CG
493 CGCG
AvaI 158 CTCGAG C^[CT]CG[GA]G
501 CTCGGG
BbvCI 245 CCTCAGC CCTCAGC(-5/-2)
115 GCTGAGG
BcnI 13 CCCGG CC^[GC]GG
264 CCCGG
BseRI 7 CTCCTC GAGGAG(10/8)
280 GAGGAG
BsoBI 158 CTCGAG C^[CT]CG[GA]G
501 CTCGGG
BspMI 149 ACCTGC ACCTGC(4/8)
568 ACCTGC
BstF5I 546 CATCC GGATG(2/0)
345 GGATG
BstUI 421 CGCG CG^CG
493 CGCG
EaeI 132 CGGCCA [CT]^GGCC[GA]
274 CGGCCG
EcoNI 65 CCTCCCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
129 CCTCGGCCAGG
FokI 546 CATCC GGATG(9/13)
345 GGATG
MvnI 421 CGCG CG^CG
493 CGCG
NciI 13 CCCGG CC^[GC]GG
264 CCCGG
NlaIII 44 CATG CATG^
58 CATG
TaqI 159 TCGA T^CGA
404 TCGA
TthHB8I 159 TCGA T^CGA
404 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 37 enzymes) #
#########################################
AciI AluI AlwNI BanI BbvI
BmyI BpmI BsaJI BsgI BsiYI
BslI Bsp1286I BssKI BstNI Cac8I
CfoI Cfr13I DdeI DraII EcoO109I
EcoRII Fnu4HI HaeIII HapII HhaI
HinP1I HpaII ItaI MnlI MspI
MvaI MwoI NlaIV Sau96I ScrFI
TseI TspRI