Restriction map for
pFN21AB6158
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# NO CUT ENZYMES ( 96 enzymes) #
##################################
AccI AclI AflII AflIII AgeI
AhdI Aor51HI AscI AseI AsnI
Asp700I AspEI AspI AvrII BamHI
BbrPI BclI BfrI BlnI BlpI
Bpu1102I BsaAI BsaBI BsiWI BsmI
BspDI BspLU11I BsrDI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstZ17I CelII ClaI CpoI DraI
DrdI Eam1105I Eco47III EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI HindIII
HpaI KspI MamI MfeI MluI
MseI MunI NaeI NcoI NgoMIV
NheI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I RsrII SacII SalI SbfI
SexAI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspI StuI SwaI
Tru9I Tth111I Van91I XbaI XhoI
XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AatII 135 GACGTC GACGT^C
Acc65I 1668 GGTACC G^GTACC
AccIII 636 TCCGGA T^CCGGA
AcsI 567 AAATTT [GA]^AATT[CT]
Alw44I 1510 GTGCAC G^TGCAC
AocI 715 CCTGAGG CC^T[ACGT]AGG
ApaLI 1510 GTGCAC G^TGCAC
ApoI 567 AAATTT [GA]^AATT[CT]
Asp718I 1668 GGTACC G^GTACC
AviII 1127 TGCGCA TGC^GCA
BbeI 149 GGCGCC GGCGC^C
BbvCI 1579 CCTCAGC CCTCAGC(-5/-2)
BcgI 89 CGAGTGCCTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1589 GTATCC GTATCC(6/5)
BfaI 1019 CTAG C^TAG
BglII 1641 AGATCT A^GATCT
BseAI 636 TCCGGA T^CCGGA
BseRI 1717 CTCCTC GAGGAG(10/8)
BsgI 337 CTGCAC GTGCAG(16/14)
Bsp1407I 749 TGTACA T^GTACA
BspEI 636 TCCGGA T^CCGGA
BspHI 1628 TCATGA T^CATGA
BsrFI 13 GCCGGT [GA]^CCGG[CT]
BsrGI 749 TGTACA T^GTACA
Bsu36I 715 CCTGAGG CC^T[ACGT]AGG
Cfr10I 13 GCCGGT [GA]^CCGG[CT]
Eco81I 715 CCTGAGG CC^T[ACGT]AGG
EcoNI 1626 CCTCATGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 944 CCAAGG C^C[AT][AT]GG
FspI 1127 TGCGCA TGC^GCA
HincII 1402 GTTGAC GT[CT]^[GA]AC
HindII 1402 GTTGAC GT[CT]^[GA]AC
KasI 149 GGCGCC G^GCGCC
KpnI 1668 GGTACC GGTAC^C
MaeI 1019 CTAG C^TAG
MroI 636 TCCGGA T^CCGGA
NarI 149 GGCGCC GG^CGCC
NdeI 1480 CATATG CA^TATG
PpuMI 551 GGGACCT [GA]G^G[AT]CC[CT]
PvuI 960 CGATCG CGAT^CG
RcaI 1628 TCATGA T^CATGA
SacI 418 GAGCTC GAGCT^C
SapI 1461 GCTCTTC GCTCTTC(1/4)
ScaI 521 AGTACT AGT^ACT
SfoI 149 GGCGCC GGC^GCC
SspBI 749 TGTACA T^GTACA
StyI 944 CCAAGG C^C[AT][AT]GG
TaqI 963 TCGA T^CGA
TthHB8I 963 TCGA T^CGA
XcmI 1442 CCAGCCGCCGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AcyI 135 GACGTC G[GA]^CG[CT]C
149 GGCGCC
ApaI 73 GGGCCC GGGCC^C
1568 GGGCCC
BalI 1140 TGGCCA TGG^CCA
1280 TGGCCA
BbsI 651 GAAGAC GAAGAC(2/6)
1216 GAAGAC
BpuAI 651 GAAGAC GAAGAC(2/6)
1216 GAAGAC
BsaHI 135 GACGTC G[GA]^CG[CT]C
149 GGCGCC
BsaWI 636 TCCGGA [AT]^CCGG[AT]
1105 TCCGGT
BsmBI 781 GAGACG CGTCTC(1/5)
799 GAGACG
BsrBI 59 CCGCTC CCGCTC(-3/-3)
1116 GAGCGG
BstXI 671 CCAACTATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1283 CCACAGTAGTGG
DraII 551 GGGACCT [GA]G^G[ACGT]CC[CT]
1568 GGGCCCT
DraIII 361 CACATCGTG CAC[ACGT][ACGT][ACGT]^GTG
1226 CACCCCGTG
EcoO109I 551 GGGACCT [GA]G^G[ACGT]CC[CT]
1568 GGGCCCT
HaeII 149 GGCGCC [GA]GCGC^[CT]
256 AGCGCC
HgaI 391 GACGC GACGC(5/10)
290 GCGTC
Hin1I 135 GACGTC G[GA]^CG[CT]C
149 GGCGCC
MaeII 136 ACGT A^CGT
802 ACGT
MluNI 1140 TGGCCA TGG^CCA
1280 TGGCCA
MscI 1140 TGGCCA TGG^CCA
1280 TGGCCA
NotI 46 GCGGCCGC GC^GGCCGC
173 GCGGCCGC
SfiI 1356 GGCCCCCCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
1485 GGCCCTGTCGGCC
SmlI 517 CTCAAG C^T[CT][GA]AG
556 CTCAAG
TfiI 1077 GAATC G^A[AT]TC
1779 GATTC
Tsp509I 568 AATT ^AATT
1318 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 88 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI AspHI AvaI AvaII BanI
BanII BbvI BcnI BglI BmyI
BpmI BsaI BsaJI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrI BssKI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DsaI EaeI
EagI EarI EclXI Eco52I EcoRII
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeIII MboI MboII
MflI MnlI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PleI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmaI TseI Tsp45I TspRI
XhoII XmaCI XmaI