Restriction map for
pFN21AB0483
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# NO CUT ENZYMES ( 129 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AlwNI Aor51HI AscI Asp718I AvaII
AviII BalI BamHI BbeI BbrPI
BbvCI BcgI BciVI BclI BcnI
BfrI BglI BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BsgI BsiEI
BsiWI BsmBI Bsp1407I BspDI BspEI
BspLU11I BsrDI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstZ17I ClaI CpoI DraI
DraIII DrdI EaeI EagI EclXI
Eco47III Eco52I EcoO65I EcoRI EcoRV
FbaI FseI FspI HaeII Hin1I
HincII HindII HindIII HpaI KasI
KpnI KspI MaeII MamI MfeI
MluI MluNI MroI MscI MunI
MvnI NaeI NarI NciI NdeI
NgoMIV NheI NotI NruI NspV
PaeR7I PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI Psp1406I PvuI
RsrII SacI SacII SalI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
Sse8387I SspBI StuI SwaI Van91I
XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AcsI 1248 AAATTC [GA]^AATT[CT]
Alw44I 151 GTGCAC G^TGCAC
ApaI 1417 GGGCCC GGGCC^C
ApaLI 151 GTGCAC G^TGCAC
ApoI 1248 AAATTC [GA]^AATT[CT]
Asp700I 18 GAATGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 151 GTGCAC G[AT]GC[AT]^C
AspI 1492 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 1539 CCTAGG C^CTAGG
BanI 1127 GGTGCC G^G[CT][GA]CC
BglII 461 AGATCT A^GATCT
BlnI 1539 CCTAGG C^CTAGG
BlpI 41 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 41 GCTCAGC GC^T[ACGT]AGC
BsiHKAI 151 GTGCAC G[AT]GC[AT]^C
BsmAI 947 GAGAC GTCTC(1/5)
BspHI 365 TCATGA T^CATGA
BspMI 920 ACCTGC ACCTGC(4/8)
BsrBI 721 GAGCGG CCGCTC(-3/-3)
BsrFI 136 ACCGGC [GA]^CCGG[CT]
BstXI 905 CCAGTGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 461 AGATCT [GA]^GATC[CT]
CelII 41 GCTCAGC GC^T[ACGT]AGC
CfoI 1555 GCGC GCG^C
Cfr10I 136 ACCGGC [GA]^CCGG[CT]
DraII 1271 AGGGCCT [GA]G^G[ACGT]CC[CT]
EarI 1456 GAAGAG CTCTTC(1/4)
EcoO109I 1271 AGGGCCT [GA]G^G[ACGT]CC[CT]
EcoT22I 425 ATGCAT ATGCA^T
FauI 66 GCGGG CCCGC(4/6)
HhaI 1555 GCGC GCG^C
HinP1I 1555 GCGC G^CGC
HphI 208 GGTGA GGTGA(8/7)
Ksp632I 1456 GAAGAG CTCTTC(1/4)
MaeIII 1504 GTCAC ^GT[ACGT]AC
MflI 461 AGATCT [GA]^GATC[CT]
MslI 254 CATGTTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI 146 CCATGG C^CATGG
NsiI 425 ATGCAT ATGCA^T
PacI 1354 TTAATTAA TTAAT^TAA
PflFI 1492 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PstI 1279 CTGCAG CTGCA^G
RcaI 365 TCATGA T^CATGA
SapI 1456 GAAGAGC GCTCTTC(1/4)
SfcI 1279 CTGCAG C^T[GA][CT]AG
SphI 427 GCATGC GCATG^C
TaqI 179 TCGA T^CGA
Tsp45I 1504 GTCAC ^GT[GC]AC
Tth111I 1492 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 179 TCGA T^CGA
XcmI 331 CCAAGAACCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 461 AGATCT [GA]^GATC[CT]
XmnI 18 GAATGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 21 enzymes) #
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AhdI 175 GACTTCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
986 GACACATTGTC
AseI 820 ATTAAT AT^TAAT
1375 ATTAAT
AsnI 820 ATTAAT AT^TAAT
1375 ATTAAT
AspEI 175 GACTTCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
986 GACACATTGTC
AvaI 1303 CTCGGG C^[CT]CG[GA]G
1444 CCCGAG
BbsI 183 GTCTTC GAAGAC(2/6)
1342 GTCTTC
BpuAI 183 GTCTTC GAAGAC(2/6)
1342 GTCTTC
BsmI 492 GAATGC GAATGC(1/-1)
924 GCATTC
BsoBI 1303 CTCGGG C^[CT]CG[GA]G
1444 CCCGAG
Eam1105I 175 GACTTCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
986 GACACATTGTC
EcoNI 1263 CCTCCTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1278 CCTGCAGAAGG
HapII 137 CCGG C^CGG
1066 CCGG
HgaI 1496 GCGTC GACGC(5/10)
1514 GCGTC
HpaII 137 CCGG C^CGG
1066 CCGG
MspA1I 1190 CAGCTG C[AC]G^C[GT]G
1228 CAGCTG
MspI 137 CCGG C^CGG
1066 CCGG
PshBI 820 ATTAAT AT^TAAT
1375 ATTAAT
PvuII 1190 CAGCTG CAG^CTG
1228 CAGCTG
SfaNI 1152 GATGC GCATC(5/9)
111 GCATC
SspI 964 AATATT AAT^ATT
1240 AATATT
TfiI 23 GATTC G^A[AT]TC
976 GATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 55 enzymes) #
#########################################
AciI AfaI AluI AlwI AocI
BanII BbvI BfaI BmyI BpmI
BsaJI BseRI BsiYI BslI BsmFI
Bsp1286I BsrI BssKI BstF5I BstNI
Bsu36I Cac8I Cfr13I DdeI DpnII
DsaI Eco81I EcoRII EcoT14I Fnu4HI
FokI HaeIII HinfI ItaI MaeI
MboI MboII MnlI MseI MvaI
MwoI NdeII NlaIII NlaIV NspI
PleI RsaI Sau3AI Sau96I ScrFI
StyI Tru9I TseI Tsp509I TspRI