Restriction map for
pFN21AE3381
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# NO CUT ENZYMES ( 96 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AgeI AlwNI AocI
Aor51HI AscI Asp700I Asp718I AvrII
BamHI BanI BbeI BbrPI BcgI
BciVI BfrI BlnI BsaAI BsaHI
BseAI BseRI BsiWI BsmI BspDI
BspEI BspHI BssHII Bst1107I BstBI
BstEII BstPI BstUI BstXI BstZ17I
Bsu36I ClaI CpoI DraI Eco47III
Eco81I EcoNI EcoO65I EcoT22I FauI
FseI HaeII HgaI Hin1I HpaI
KasI KpnI KspI MfeI MluI
MroI MunI MvnI NarI NdeI
NheI NotI NruI NsiI NspV
PacI PinAI PmaCI PmeI PmlI
Psp1406I PvuI RcaI RsrII SacII
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SwaI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AccI 347 GTCGAC GT^[AC][GT]AC
AflIII 3444 ACATGT A^C[GA][CT]GT
ApaI 1482 GGGCCC GGGCC^C
AseI 1987 ATTAAT AT^TAAT
AsnI 1987 ATTAAT AT^TAAT
AspI 2529 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 929 CTCGAG C^[CT]CG[GA]G
AviII 2198 TGCGCA TGC^GCA
BalI 1797 TGGCCA TGG^CCA
BclI 1040 TGATCA T^GATCA
BglI 1103 GCCATTTCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 2845 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2845 GCTCAGC GC^T[ACGT]AGC
BsaBI 1681 GATAATTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 843 TCCGGT [AT]^CCGG[AT]
BsiEI 40 CGGCCG CG[GA][CT]^CG
BsmBI 2534 CGTCTC CGTCTC(1/5)
BsoBI 929 CTCGAG C^[CT]CG[GA]G
BspLU11I 3444 ACATGT A^CATGT
BspMI 3159 ACCTGC ACCTGC(4/8)
BsrDI 2853 CATTGC GCAATG(2/0)
BstAPI 2567 GCACTGTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 2845 GCTCAGC GC^T[ACGT]AGC
DrdI 2526 GACGACCACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 40 CGGCCG C^GGCCG
EclXI 40 CGGCCG C^GGCCG
Eco52I 40 CGGCCG C^GGCCG
FbaI 1040 TGATCA T^GATCA
FspI 2198 TGCGCA TGC^GCA
MamI 1681 GATAATTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 1797 TGGCCA TGG^CCA
MscI 1797 TGGCCA TGG^CCA
MslI 2452 CATTCCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
PaeR7I 929 CTCGAG C^TCGAG
PflFI 2529 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 434 AGGTCCC [GA]G^G[AT]CC[CT]
PshAI 2656 GACCTGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 1987 ATTAAT AT^TAAT
SalI 347 GTCGAC G^TCGAC
SapI 577 GAAGAGC GCTCTTC(1/4)
StuI 2254 AGGCCT AGG^CCT
Tth111I 2529 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 936 TCTAGA T^CTAGA
XhoI 929 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 36 enzymes) #
###################################
AhdI 241 GACACACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
450 GACTGCTGGTC
AlwI 1843 GATCC GGATC(4/5)
3233 GGATC
AspEI 241 GACACACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
450 GACTGCTGGTC
BglII 1544 AGATCT A^GATCT
2490 AGATCT
BsaI 1356 GAGACC GGTCTC(1/5)
3156 GAGACC
BsgI 659 CTGCAC GTGCAG(16/14)
2639 CTGCAC
Bsp1407I 521 TGTACA T^GTACA
547 TGTACA
BsrBI 784 GAGCGG CCGCTC(-3/-3)
2521 GAGCGG
BsrFI 2090 GCCGGC [GA]^CCGG[CT]
3362 GCCGGC
BsrGI 521 TGTACA T^GTACA
547 TGTACA
BssSI 2560 CACGAG CACGAG(-5/-1)
3228 CTCGTG
Cfr10I 2090 GCCGGC [GA]^CCGG[CT]
3362 GCCGGC
DraII 434 AGGTCCC [GA]G^G[ACGT]CC[CT]
1482 GGGCCCT
DraIII 956 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
2568 CACTGTGTG
DsaI 704 CCATGG C^C[GA][CT]GG
1523 CCATGG
EaeI 40 CGGCCG [CT]^GGCC[GA]
1797 TGGCCA
Eam1105I 241 GACACACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
450 GACTGCTGGTC
EarI 577 GAAGAG CTCTTC(1/4)
2154 GAAGAG
EcoO109I 434 AGGTCCC [GA]G^G[ACGT]CC[CT]
1482 GGGCCCT
HincII 347 GTCGAC GT[CT]^[GA]AC
2663 GTCAAC
HindII 347 GTCGAC GT[CT]^[GA]AC
2663 GTCAAC
Ksp632I 577 GAAGAG CTCTTC(1/4)
2154 GAAGAG
MseI 1900 TTAA T^TAA
1988 TTAA
MspA1I 682 CAGCTG C[AC]G^C[GT]G
1826 CAGCTG
NaeI 2090 GCCGGC GCC^GGC
3362 GCCGGC
NcoI 704 CCATGG C^CATGG
1523 CCATGG
NgoMIV 2090 GCCGGC G^CCGGC
3362 GCCGGC
NspI 3432 ACATGC [GA]CATG^[CT]
3444 ACATGT
PflMI 2947 CCATATTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3343 CCAACCAGTGG
PstI 793 CTGCAG CTGCA^G
816 CTGCAG
PvuII 682 CAGCTG CAG^CTG
1826 CAGCTG
SspBI 521 TGTACA T^GTACA
547 TGTACA
SspI 1459 AATATT AAT^ATT
3356 AATATT
Tru9I 1900 TTAA T^TAA
1988 TTAA
Van91I 2947 CCATATTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3343 CCAACCAGTGG
XcmI 461 CCAAGTGGTTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2540 CCAAAAGGCTGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 82 enzymes) #
#########################################
AciI AcsI AfaI AluI Alw44I
ApaLI ApoI AspHI AvaII BanII
BbsI BbvCI BbvI BcnI BfaI
BmyI BpmI BpuAI BsaJI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
EcoRI EcoRII EcoRV EcoT14I Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HindIII HinfI HpaII HphI ItaI
MaeI MaeII MaeIII MboI MboII
MflI MnlI MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PleI
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII