Restriction map for
pFN21AE3078
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# NO CUT ENZYMES ( 164 enzymes) #
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AatII Acc65I AccII AccIII AclI
AflII AflIII AgeI AhdI Alw44I
AlwI AocI Aor51HI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AvaI AvrII BalI BamHI
BanI BbeI BbrPI BcgI BciVI
BclI BfaI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaI BsaWI BseAI BsgI BsiEI
BsiHKAI BsiWI BsmAI BsmBI BsmFI
BsmI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrFI BsrGI BssHII
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstXI BstYI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DraI
DraIII DrdI DsaI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoRV EcoT14I EcoT22I FbaI FseI
HincII HindII HindIII HinfI HpaI
KasI KpnI KspI MaeI MaeII
MamI MfeI MflI MluI MluNI
MroI MscI MslI MunI MvnI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PinAI PleI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI StyI
SwaI TfiI XbaI XcmI XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AccI 457 GTCTAC GT^[AC][GT]AC
AcyI 31 GACGCC G[GA]^CG[CT]C
AfaI 253 GTAC GT^AC
ApaI 514 GGGCCC GGGCC^C
AspI 451 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 59 GGACC G^G[AT]CC
AviII 605 TGCGCA TGC^GCA
BbsI 448 GAAGAC GAAGAC(2/6)
BbvCI 144 CCTCAGC CCTCAGC(-5/-2)
BcnI 480 CCGGG CC^[GC]GG
BpmI 22 CTGGAG CTGGAG(16/14)
BpuAI 448 GAAGAC GAAGAC(2/6)
BsaHI 31 GACGCC G[GA]^CG[CT]C
BspMI 497 ACCTGC ACCTGC(4/8)
BsrBI 96 CCGCTC CCGCTC(-3/-3)
BsrDI 317 GCAATG GCAATG(2/0)
BssSI 397 CTCGTG CACGAG(-5/-1)
BstF5I 487 CATCC GGATG(2/0)
EaeI 102 CGGCCA [CT]^GGCC[GA]
EarI 648 GAAGAG CTCTTC(1/4)
EcoRI 219 GAATTC G^AATTC
FauI 95 CCCGC CCCGC(4/6)
FokI 487 CATCC GGATG(9/13)
FspI 605 TGCGCA TGC^GCA
HgaI 31 GACGC GACGC(5/10)
Hin1I 31 GACGCC G[GA]^CG[CT]C
HphI 50 TCACC GGTGA(8/7)
Ksp632I 648 GAAGAG CTCTTC(1/4)
MseI 248 TTAA T^TAA
NciI 480 CCGGG CC^[GC]GG
NlaIII 553 CATG CATG^
NlaIV 514 GGGCCC GG[ACGT]^[ACGT]CC
PflFI 451 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 289 CCAAATTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 58 AGGACCT [GA]G^G[AT]CC[CT]
PstI 637 CTGCAG CTGCA^G
PvuII 634 CAGCTG CAG^CTG
RsaI 253 GTAC GT^AC
SfaNI 608 GCATC GCATC(5/9)
TaqI 374 TCGA T^CGA
Tru9I 248 TTAA T^TAA
Tth111I 451 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 374 TCGA T^CGA
Van91I 289 CCAAATTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AlwNI 640 CAGAACCTG CAG[ACGT][ACGT][ACGT]^CTG
673 CAGAAGCTG
BanII 408 GAGCCC G[GA]GC[CT]^C
514 GGGCCC
BmyI 408 GAGCCC G[AGT]GC[ACT]^C
514 GGGCCC
BseRI 25 GAGGAG GAGGAG(10/8)
154 GAGGAG
Bsp1286I 408 GAGCCC G[AGT]GC[ACT]^C
514 GGGCCC
BsrI 238 ACTGG ACTGG(1/-1)
311 CCAGT
DpnII 159 GATC ^GATC
372 GATC
HaeII 78 AGCGCC [GA]GCGC^[CT]
653 GGCGCT
HapII 101 CCGG C^CGG
480 CCGG
HpaII 101 CCGG C^CGG
480 CCGG
MboI 159 GATC ^GATC
372 GATC
MspA1I 6 CAGCGG C[AC]G^C[GT]G
634 CAGCTG
MspI 101 CCGG C^CGG
480 CCGG
NdeII 159 GATC ^GATC
372 GATC
Sau3AI 159 GATC ^GATC
372 GATC
TspRI 237 CACTG CA[GC]TG[ACGT][ACGT]^
357 CACTG
#########################################
# 3 OR MORE CUT ENZYMES ( 34 enzymes) #
#########################################
AciI AcsI AluI ApoI BbvI
BsaJI BsiYI BslI BssKI BstNI
Cac8I CfoI Cfr13I DdeI DraII
EcoNI EcoO109I EcoRII Fnu4HI HaeIII
HhaI HinP1I ItaI MaeIII MboII
MnlI MvaI MwoI Sau96I ScrFI
SfcI TseI Tsp45I Tsp509I