Restriction map for
pFN21AE3541
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# NO CUT ENZYMES ( 133 enzymes) #
##################################
AatII AccIII AclI AcsI AflII
AflIII AgeI AhdI Alw44I AlwI
AlwNI AocI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
AspEI AspHI AspI AvaI AviII
BamHI BbrPI BbsI BbvCI BcgI
BclI BcnI BfrI BglII BpuAI
BsaBI BsaWI BseAI BsiHKAI BsiWI
BsmBI BsmI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstYI BstZ17I Bsu36I ClaI DraI
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HincII HindII HindIII HpaI MamI
MfeI MflI MluI MroI MseI
MunI NciI NcoI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PleI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
RcaI SacI SalI SapI SbfI
ScaI SexAI SfiI SfuI SgrAI
SmaI SmlI SnaBI SpeI Sse8387I
SspBI SspI StuI SwaI Tru9I
Tsp509I Tth111I XbaI XhoI XhoII
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
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Acc65I 358 GGTACC G^GTACC
Asp718I 358 GGTACC G^GTACC
AvrII 429 CCTAGG C^CTAGG
BalI 653 TGGCCA TGG^CCA
BanII 241 GAGCCC G[GA]GC[CT]^C
BciVI 851 GTATCC GTATCC(6/5)
BfaI 430 CTAG C^TAG
BlnI 429 CCTAGG C^CTAGG
BlpI 546 GCTCAGC GC^T[ACGT]AGC
BmyI 241 GAGCCC G[AGT]GC[ACT]^C
BpmI 238 CTGGAG CTGGAG(16/14)
Bpu1102I 546 GCTCAGC GC^T[ACGT]AGC
BsaAI 216 CACGTA [CT]AC^GT[GA]
BseRI 175 GAGGAG GAGGAG(10/8)
BsgI 152 CTGCAC GTGCAG(16/14)
Bsp1286I 241 GAGCCC G[AGT]GC[ACT]^C
BsrBI 544 CCGCTC CCGCTC(-3/-3)
BsrDI 230 GCAATG GCAATG(2/0)
BstAPI 758 GCATGCACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 546 GCTCAGC GC^T[ACGT]AGC
CpoI 540 CGGACCG CG^G[AT]CCG
DraIII 323 CACTCCGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 34 CTCTTC CTCTTC(1/4)
EcoT14I 429 CCTAGG C^C[AT][AT]GG
KpnI 358 GGTACC GGTAC^C
Ksp632I 34 CTCTTC CTCTTC(1/4)
MaeI 430 CTAG C^TAG
MluNI 653 TGGCCA TGG^CCA
MscI 653 TGGCCA TGG^CCA
PflMI 160 CCAAGTAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 75 GGGACCC [GA]G^G[AT]CC[CT]
PvuI 461 CGATCG CGAT^CG
PvuII 807 CAGCTG CAG^CTG
RsrII 540 CGGACCG CG^G[AT]CCG
StyI 429 CCTAGG C^C[AT][AT]GG
TaqI 455 TCGA T^CGA
TthHB8I 455 TCGA T^CGA
Van91I 160 CCAAGTAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 160 CCAAGTAATGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccI 382 GTCTAC GT^[AC][GT]AC
616 GTCTAC
AfaI 219 GTAC GT^AC
359 GTAC
BbeI 349 GGCGCC GGCGC^C
769 GGCGCC
BglI 127 GCCTCCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
428 GCCTAGGCGGC
BsaI 4 GAGACC GGTCTC(1/5)
370 GAGACC
BsiEI 201 CGACCG CG[GA][CT]^CG
461 CGATCG
BspMI 7 ACCTGC ACCTGC(4/8)
361 ACCTGC
DdeI 398 CTGAG C^T[ACGT]AG
547 CTCAG
DraII 75 GGGACCC [GA]G^G[ACGT]CC[CT]
647 GGGGCCT
EcoO109I 75 GGGACCC [GA]G^G[ACGT]CC[CT]
647 GGGGCCT
HgaI 420 GCGTC GACGC(5/10)
614 GCGTC
HinfI 118 GATTC G^A[ACGT]TC
821 GATTC
HphI 499 GGTGA GGTGA(8/7)
835 GGTGA
KasI 349 GGCGCC G^GCGCC
769 GGCGCC
KspI 288 CCGCGG CCGC^GG
353 CCGCGG
MaeIII 329 GTGAC ^GT[ACGT]AC
836 GTGAC
MboII 35 TCTTC GAAGA(8/7)
302 TCTTC
NaeI 440 GCCGGC GCC^GGC
551 GCCGGC
NarI 349 GGCGCC GG^CGCC
769 GGCGCC
NgoMIV 440 GCCGGC G^CCGGC
551 GCCGGC
NlaIII 590 CATG CATG^
759 CATG
NspI 589 GCATGC [GA]CATG^[CT]
758 GCATGC
RsaI 219 GTAC GT^AC
359 GTAC
SacII 288 CCGCGG CCGC^GG
353 CCGCGG
SfoI 349 GGCGCC GGC^GCC
769 GGCGCC
SphI 589 GCATGC GCATG^C
758 GCATGC
TfiI 118 GATTC G^A[AT]TC
821 GATTC
Tsp45I 329 GTGAC ^GT[GC]AC
836 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 58 enzymes) #
#########################################
AccII AciI AcyI AluI AvaII
BanI BbvI BsaHI BsaJI BsiYI
BslI BsmAI BsmFI BsrFI BsrI
BssKI BstF5I BstNI BstUI BstXI
Cac8I CfoI Cfr10I Cfr13I DpnII
DsaI EaeI EcoRII FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
Hin1I HinP1I HpaII ItaI MaeII
MboI MnlI MslI MspA1I MspI
MvaI MvnI MwoI NdeII NlaIV
PstI Sau3AI Sau96I ScrFI SfaNI
SfcI TseI TspRI