Restriction map for
pFN21AE4137
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# NO CUT ENZYMES ( 153 enzymes) #
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AatII AccII AccIII AclI AcyI
AflII AgeI AhdI AlwI AlwNI
AocI Aor51HI ApaI AscI AseI
AsnI AspEI AspI AvaI AvaII
AviII AvrII BalI BamHI BbeI
BbrPI BbvCI BciVI BcnI BfaI
BfrI BglII BlnI BlpI Bpu1102I
BsaBI BsaHI BsaI BsaWI BseAI
BsgI BsiWI BsoBI Bsp1407I BspDI
BspEI BspHI BsrBI BsrDI BsrFI
BsrGI BssHII Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstYI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DraI DraII DraIII DrdI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRV
EcoT22I FauI FseI FspI HaeII
HapII HgaI Hin1I HpaI HpaII
KasI KspI MaeI MamI MfeI
MflI MluI MluNI MroI MscI
MseI MspI MunI MvnI NaeI
NarI NciI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PpuMI PshBI Psp1406I RcaI
RsrII SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
Tru9I Tth111I XbaI XcmI XhoI
XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 35 enzymes) #
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Acc65I 199 GGTACC G^GTACC
AccI 242 GTAGAC GT^[AC][GT]AC
AcsI 794 GAATTC [GA]^AATT[CT]
AflIII 726 ACATGT A^C[GA][CT]GT
Alw44I 452 GTGCAC G^TGCAC
ApaLI 452 GTGCAC G^TGCAC
ApoI 794 GAATTC [GA]^AATT[CT]
Asp718I 199 GGTACC G^GTACC
BanI 199 GGTACC G^G[CT][GA]CC
BbsI 580 GAAGAC GAAGAC(2/6)
BglI 1159 GCCCCTGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 580 GAAGAC GAAGAC(2/6)
BsaAI 962 TACGTG [CT]AC^GT[GA]
BsiEI 549 CGATCG CG[GA][CT]^CG
BsmBI 771 CGTCTC CGTCTC(1/5)
BsmI 837 GAATGC GAATGC(1/-1)
BspLU11I 726 ACATGT A^CATGT
BssSI 535 CTCGTG CACGAG(-5/-1)
BstXI 530 CCATCCTCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr13I 219 GGGCC G^G[ACGT]CC
EcoRI 794 GAATTC G^AATTC
HincII 934 GTCAAC GT[CT]^[GA]AC
HindII 934 GTCAAC GT[CT]^[GA]AC
HindIII 1124 AAGCTT A^AGCTT
HphI 111 GGTGA GGTGA(8/7)
KpnI 199 GGTACC GGTAC^C
MaeIII 1228 GTGAC ^GT[ACGT]AC
NspI 726 ACATGT [GA]CATG^[CT]
PflMI 1279 CCAGCGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuI 549 CGATCG CGAT^CG
PvuII 574 CAGCTG CAG^CTG
Sau96I 219 GGGCC G^G[ACGT]CC
StuI 167 AGGCCT AGG^CCT
Tsp45I 1228 GTGAC ^GT[GC]AC
Van91I 1279 CCAGCGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 23 enzymes) #
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AfaI 200 GTAC GT^AC
421 GTAC
Asp700I 921 GAACCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1078 GAAAATGTTC
BanII 511 GAGCTC G[GA]GC[CT]^C
1132 GAGCTC
BcgI 223 CGATTCCGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
590 GCAAAACTATCG
BsmAI 498 GAGAC GTCTC(1/5)
772 GTCTC
BsmFI 1263 GGGAC GGGAC(10/14)
77 GTCCC
CfoI 5 GCGC GCG^C
390 GCGC
DsaI 47 CCATGG C^C[GA][CT]GG
206 CCATGG
EarI 382 GAAGAG CTCTTC(1/4)
1069 GAAGAG
EcoT14I 47 CCATGG C^C[AT][AT]GG
206 CCATGG
HhaI 5 GCGC GCG^C
390 GCGC
HinP1I 5 GCGC G^CGC
390 GCGC
Ksp632I 382 GAAGAG CTCTTC(1/4)
1069 GAAGAG
MaeII 770 ACGT A^CGT
963 ACGT
MspA1I 228 CCGCTG C[AC]G^C[GT]G
574 CAGCTG
NcoI 47 CCATGG C^CATGG
206 CCATGG
PshAI 500 GACAGAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
687 GACCCAAGTC
RsaI 200 GTAC GT^AC
421 GTAC
SacI 511 GAGCTC GAGCT^C
1132 GAGCTC
StyI 47 CCATGG C^C[AT][AT]GG
206 CCATGG
TaqI 552 TCGA T^CGA
599 TCGA
TthHB8I 552 TCGA T^CGA
599 TCGA
XmnI 921 GAACCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1078 GAAAATGTTC
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# 3 OR MORE CUT ENZYMES ( 47 enzymes) #
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AciI AluI AspHI BbvI BclI
BmyI BpmI BsaJI BseRI BsiHKAI
BsiYI BslI Bsp1286I BspMI BsrI
BssKI BstF5I BstNI Cac8I DdeI
DpnII EcoRII FbaI Fnu4HI FokI
HaeIII HinfI ItaI MboI MboII
MnlI MslI MvaI MwoI NdeII
NlaIII NlaIV PleI PstI Sau3AI
ScrFI SfaNI SfcI TfiI TseI
Tsp509I TspRI