Restriction map for
pFN21AB9976
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# NO CUT ENZYMES ( 99 enzymes) #
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AatII Acc65I AclI AflII AflIII
AgeI AhdI Alw44I AlwNI ApaLI
AscI AseI AsnI Asp718I AspEI
AspHI AviII AvrII BalI BbrPI
BbvCI BciVI BclI BfrI BlnI
BlpI Bpu1102I BsaAI BsaI BsgI
BsiHKAI BsiWI BsmI Bsp1407I BspDI
BspLU11I BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstZ17I CelII Cfr10I ClaI CpoI
DraIII DrdI Eam1105I EcoO65I EcoT22I
FbaI FseI FspI HpaI KpnI
MluI MluNI MscI NaeI NdeI
NgoMIV NheI NruI NsiI NspV
PacI PaeR7I PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SexAI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspBI SwaI Van91I XhoI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AccI 1076 GTAGAC GT^[AC][GT]AC
AccIII 17 TCCGGA T^CCGGA
AocI 535 CCTCAGG CC^T[ACGT]AGG
Aor51HI 1994 AGCGCT AGC^GCT
Asp700I 1648 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 2030 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 1809 GGATCC G^GATCC
BbsI 1373 GTCTTC GAAGAC(2/6)
BpuAI 1373 GTCTTC GAAGAC(2/6)
BsaBI 1573 GATGCCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 17 TCCGGA [AT]^CCGG[AT]
BseAI 17 TCCGGA T^CCGGA
BsmBI 1620 GAGACG CGTCTC(1/5)
BspEI 17 TCCGGA T^CCGGA
BspHI 1581 TCATGA T^CATGA
BspMI 912 ACCTGC ACCTGC(4/8)
BsrDI 1849 GCAATG GCAATG(2/0)
BstXI 48 CCACAGGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 535 CCTCAGG CC^T[ACGT]AGG
DraI 832 TTTAAA TTT^AAA
Eco47III 1994 AGCGCT AGC^GCT
Eco81I 535 CCTCAGG CC^T[ACGT]AGG
EcoNI 1897 CCTCTAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 934 GAATTC G^AATTC
EcoRV 919 GATATC GAT^ATC
EcoT14I 137 CCATGG C^C[AT][AT]GG
HgaI 1640 GACGC GACGC(5/10)
HincII 1742 GTCAAC GT[CT]^[GA]AC
HindII 1742 GTCAAC GT[CT]^[GA]AC
HindIII 1012 AAGCTT A^AGCTT
MamI 1573 GATGCCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1690 CAATTG C^AATTG
MroI 17 TCCGGA T^CCGGA
MunI 1690 CAATTG C^AATTG
NcoI 137 CCATGG C^CATGG
PflFI 2030 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PstI 464 CTGCAG CTGCA^G
RcaI 1581 TCATGA T^CATGA
SmlI 852 CTTGAG C^T[CT][GA]AG
SspI 1466 AATATT AAT^ATT
StuI 859 AGGCCT AGG^CCT
StyI 137 CCATGG C^C[AT][AT]GG
TspRI 1293 CAGTG CA[GC]TG[ACGT][ACGT]^
Tth111I 2030 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 1021 TCTAGA T^CTAGA
XmnI 1648 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 22 enzymes) #
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AcyI 489 GGCGCC G[GA]^CG[CT]C
1769 GGCGCC
BanI 489 GGCGCC G^G[CT][GA]CC
1769 GGCGCC
BbeI 489 GGCGCC GGCGC^C
1769 GGCGCC
BcgI 466 GCAGTCACCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
992 GCAAAGGATTCG
BsaHI 489 GGCGCC G[GA]^CG[CT]C
1769 GGCGCC
BsiEI 69 CGGCCG CG[GA][CT]^CG
795 CGGCCG
BsrBI 350 CCGCTC CCGCTC(-3/-3)
159 GAGCGG
BsrI 1053 ACTGG ACTGG(1/-1)
1330 CCAGT
EagI 69 CGGCCG C^GGCCG
795 CGGCCG
EclXI 69 CGGCCG C^GGCCG
795 CGGCCG
Eco52I 69 CGGCCG C^GGCCG
795 CGGCCG
Hin1I 489 GGCGCC G[GA]^CG[CT]C
1769 GGCGCC
KasI 489 GGCGCC G^GCGCC
1769 GGCGCC
NarI 489 GGCGCC GG^CGCC
1769 GGCGCC
NotI 68 GCGGCCGC GC^GGCCGC
794 GCGGCCGC
SfcI 464 CTGCAG C^T[GA][CT]AG
1229 CTACAG
SfiI 70 GGCCGCGGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
154 GGCCAGAGCGGCC
SfoI 489 GGCGCC GGC^GCC
1769 GGCGCC
TaqI 705 TCGA T^CGA
1086 TCGA
Tsp45I 469 GTCAC ^GT[GC]AC
1979 GTGAC
TthHB8I 705 TCGA T^CGA
1086 TCGA
XcmI 1043 CCAAAGCCGAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1148 CCAATGAACTGTTGG
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# 3 OR MORE CUT ENZYMES ( 91 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI ApaI ApoI AvaI AvaII
BanII BbvI BcnI BfaI BglI
BglII BmyI BpmI BsaJI BseRI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BssKI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoO109I EcoRII FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
KspI MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV NspI
PleI PpuMI RsaI SacII Sau3AI
Sau96I ScrFI SfaNI SmaI TfiI
Tru9I TseI Tsp509I XhoII XmaCI
XmaI