Restriction map for
pFN21AE4168
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# NO CUT ENZYMES ( 114 enzymes) #
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AatII Acc65I AccIII AclI AcyI
AflII AgeI Alw44I AocI ApaI
ApaLI AscI AseI AsnI Asp718I
AvaI AviII AvrII BbeI BbrPI
BcgI BfrI BlnI BlpI Bpu1102I
BsaAI BsaBI BsaHI BsaI BseAI
BsgI BsiWI BsmBI BsoBI BspEI
BspHI BspLU11I BsrBI BsrFI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstZ17I Bsu36I CelII Cfr10I CpoI
DraI DrdI EagI EclXI Eco52I
Eco81I EcoO65I EcoRI EcoT22I FseI
FspI HgaI Hin1I HpaI KasI
KpnI KspI MamI MfeI MluI
MroI MunI NaeI NarI NdeI
NgoMIV NotI NruI NsiI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI Sse8387I SspI StuI SwaI
XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 58 enzymes) #
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AccI 1453 GTCTAC GT^[AC][GT]AC
AccII 895 CGCG CG^CG
AcsI 154 AAATTC [GA]^AATT[CT]
AhdI 1098 GACTGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 90 AGCGCT AGC^GCT
ApoI 154 AAATTC [GA]^AATT[CT]
Asp700I 538 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1098 GACTGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 676 GAGCTC G[AT]GC[AT]^C
AspI 76 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 842 GGACC G^G[AT]CC
BalI 1605 TGGCCA TGG^CCA
BamHI 1529 GGATCC G^GATCC
BanII 676 GAGCTC G[GA]GC[CT]^C
BbsI 1337 GTCTTC GAAGAC(2/6)
BbvCI 142 GCTGAGG CCTCAGC(-5/-2)
BclI 939 TGATCA T^GATCA
BglI 822 GCCTGTCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 617 AGATCT A^GATCT
BmyI 676 GAGCTC G[AGT]GC[ACT]^C
BpuAI 1337 GTCTTC GAAGAC(2/6)
BsaWI 1526 ACCGGA [AT]^CCGG[AT]
BseRI 105 GAGGAG GAGGAG(10/8)
BsiEI 588 CGACCG CG[GA][CT]^CG
BsiHKAI 676 GAGCTC G[AT]GC[AT]^C
BsmAI 763 GAGAC GTCTC(1/5)
BsmI 948 GAATGC GAATGC(1/-1)
Bsp1286I 676 GAGCTC G[AGT]GC[ACT]^C
BspDI 490 ATCGAT AT^CGAT
BspMI 1595 ACCTGC ACCTGC(4/8)
BstUI 895 CGCG CG^CG
BstXI 992 CCATGCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
ClaI 490 ATCGAT AT^CGAT
DraII 788 AGGCCCT [GA]G^G[ACGT]CC[CT]
DraIII 256 CACGGTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 1098 GACTGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 90 AGCGCT AGC^GCT
EcoNI 564 CCTGCTTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 788 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoRV 1347 GATATC GAT^ATC
FbaI 939 TGATCA T^GATCA
HaeII 90 AGCGCT [GA]GCGC^[CT]
HincII 781 GTCAAC GT[CT]^[GA]AC
HindII 781 GTCAAC GT[CT]^[GA]AC
HindIII 1483 AAGCTT A^AGCTT
MluNI 1605 TGGCCA TGG^CCA
MscI 1605 TGGCCA TGG^CCA
MvnI 895 CGCG CG^CG
PflFI 76 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 834 GACTC GAGTC(4/5)
PstI 1441 CTGCAG CTGCA^G
PvuII 367 CAGCTG CAG^CTG
SacI 676 GAGCTC GAGCT^C
TaqI 491 TCGA T^CGA
Tth111I 76 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 491 TCGA T^CGA
XcmI 1608 CCAAAATGAATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 538 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 19 enzymes) #
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AflIII 1084 ACGTGT A^C[GA][CT]GT
1579 ACACGT
BanI 1180 GGTGCC G^G[CT][GA]CC
1852 GGCACC
BciVI 714 GGATAC GTATCC(6/5)
1694 GTATCC
BcnI 201 CCGGG CC^[GC]GG
1161 CCGGG
BsmFI 1034 GGGAC GGGAC(10/14)
738 GTCCC
Bsp1407I 440 TGTACA T^GTACA
1473 TGTACA
BsrGI 440 TGTACA T^GTACA
1473 TGTACA
BstAPI 116 GCATCCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1167 GCATTTTGTGC
DsaI 1184 CCATGG C^C[GA][CT]GG
1463 CCATGG
EaeI 1077 TGGCCG [CT]^GGCC[GA]
1605 TGGCCA
FauI 1118 CCCGC CCCGC(4/6)
352 GCGGG
HphI 131 TCACC GGTGA(8/7)
360 TCACC
MaeII 1084 ACGT A^CGT
1581 ACGT
MspA1I 350 CAGCGG C[AC]G^C[GT]G
367 CAGCTG
NciI 201 CCGGG CC^[GC]GG
1161 CCGGG
NcoI 1184 CCATGG C^CATGG
1463 CCATGG
NheI 345 GCTAGC G^CTAGC
627 GCTAGC
SfaNI 116 GCATC GCATC(5/9)
809 GCATC
SspBI 440 TGTACA T^GTACA
1473 TGTACA
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# 3 OR MORE CUT ENZYMES ( 67 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
BbvI BfaI BpmI BsaJI BsiYI
BslI BsrDI BsrI BssKI BstF5I
BstNI BstYI Cac8I CfoI Cfr13I
DdeI DpnII EarI EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII ItaI Ksp632I
MaeI MaeIII MboI MboII MflI
MnlI MseI MslI MspI MvaI
MwoI NdeII NlaIII NlaIV NspI
PflMI RsaI SapI Sau3AI Sau96I
ScrFI SfcI SphI StyI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
Van91I XhoII