Restriction map for
pFN21AE3508
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# NO CUT ENZYMES ( 107 enzymes) #
##################################
AatII AccI AccIII AclI AcsI
AcyI AflII AgeI AhdI Alw44I
ApaLI ApoI AscI AseI AsnI
Asp700I AspEI AvrII BamHI BbeI
BbsI BcgI BclI BfrI BglII
BlnI BpuAI BsaBI BsaHI BsaWI
BseAI BsiWI BsmBI BsmI Bsp1407I
BspDI BspEI BspHI BsrGI BssHII
Bst1107I BstBI BstZ17I ClaI CpoI
DraI DrdI Eam1105I EarI EcoRI
EcoRV EcoT22I FbaI FseI Hin1I
HincII HindII HindIII HpaI KasI
Ksp632I KspI MamI MfeI MluI
MroI MseI MunI NarI NdeI
NotI NruI NsiI NspV PacI
PinAI PmeI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI TfiI Tru9I XbaI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 31 enzymes) #
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Acc65I 188 GGTACC G^GTACC
AflIII 1571 ACATGT A^C[GA][CT]GT
AocI 569 CCTCAGG CC^T[ACGT]AGG
Aor51HI 1515 AGCGCT AGC^GCT
Asp718I 188 GGTACC G^GTACC
AviII 1253 TGCGCA TGC^GCA
BalI 1005 TGGCCA TGG^CCA
BbvCI 711 CCTCAGC CCTCAGC(-5/-2)
BciVI 1337 GGATAC GTATCC(6/5)
BlpI 1740 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1740 GCTGAGC GC^T[ACGT]AGC
BspLU11I 1571 ACATGT A^CATGT
BsrDI 836 CATTGC GCAATG(2/0)
BssSI 415 CTCGTG CACGAG(-5/-1)
BstAPI 835 GCATTGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 569 CCTCAGG CC^T[ACGT]AGG
CelII 1740 GCTGAGC GC^T[ACGT]AGC
DraIII 1840 CACGGCGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 1515 AGCGCT AGC^GCT
Eco81I 569 CCTCAGG CC^T[ACGT]AGG
FspI 1253 TGCGCA TGC^GCA
KpnI 188 GGTACC GGTAC^C
MluNI 1005 TGGCCA TGG^CCA
MscI 1005 TGGCCA TGG^CCA
NaeI 1415 GCCGGC GCC^GGC
NgoMIV 1415 GCCGGC G^CCGGC
NheI 553 GCTAGC G^CTAGC
PaeR7I 511 CTCGAG C^TCGAG
Tsp509I 137 AATT ^AATT
XcmI 518 CCAGCTATGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 511 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 34 enzymes) #
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AccII 27 CGCG CG^CG
1584 CGCG
AspI 634 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2050 GACAAGGTC
BbrPI 291 CACGTG CAC^GTG
403 CACGTG
BglI 566 GCCCCTCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
575 GCCCCAAGGGC
BsaAI 291 CACGTG [CT]AC^GT[GA]
403 CACGTG
BsiEI 1544 CGGCCG CG[GA][CT]^CG
1948 CGGCCG
BsrBI 2094 CCGCTC CCGCTC(-3/-3)
1507 GAGCGG
BstEII 639 GGTCACC G^GT[ACGT]ACC
1641 GGTGACC
BstPI 639 GGTCACC G^GT[ACGT]ACC
1641 GGTGACC
BstUI 27 CGCG CG^CG
1584 CGCG
BstYI 168 GGATCT [GA]^GATC[CT]
725 AGATCC
EagI 1544 CGGCCG C^GGCCG
1948 CGGCCG
EclXI 1544 CGGCCG C^GGCCG
1948 CGGCCG
Eco52I 1544 CGGCCG C^GGCCG
1948 CGGCCG
EcoO65I 639 GGTCACC G^GT[ACGT]ACC
1641 GGTGACC
HaeII 1515 AGCGCT [GA]GCGC^[CT]
2033 GGCGCT
HgaI 1293 GACGC GACGC(5/10)
605 GCGTC
MboII 1686 GAAGA GAAGA(8/7)
1180 TCTTC
MflI 168 GGATCT [GA]^GATC[CT]
725 AGATCC
MslI 1311 CACCAAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1909 CACACCAATG
MvnI 27 CGCG CG^CG
1584 CGCG
NspI 1305 GCATGT [GA]CATG^[CT]
1571 ACATGT
PflFI 634 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2050 GACAAGGTC
PflMI 58 CCAGAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2073 CCACAAGCTGG
PmaCI 291 CACGTG CAC^GTG
403 CACGTG
PmlI 291 CACGTG CAC^GTG
403 CACGTG
PpuMI 34 GGGTCCC [GA]G^G[AT]CC[CT]
2054 AGGTCCT
PstI 815 CTGCAG CTGCA^G
1930 CTGCAG
SacI 148 GAGCTC GAGCT^C
1954 GAGCTC
TaqI 512 TCGA T^CGA
2048 TCGA
Tth111I 634 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2050 GACAAGGTC
TthHB8I 512 TCGA T^CGA
2048 TCGA
Van91I 58 CCAGAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2073 CCACAAGCTGG
XhoII 168 GGATCT [GA]^GATC[CT]
725 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 86 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
ApaI AspHI AvaI AvaII BanI
BanII BbvI BcnI BfaI BmyI
BpmI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrFI BsrI
BssKI BstF5I BstNI BstXI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI MaeI MaeII
MaeIII MboI MnlI MspA1I MspI
MvaI MwoI NciI NcoI NdeII
NlaIII NlaIV PleI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StuI StyI TseI Tsp45I
TspRI