Restriction map for
pFN21AE4286
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# NO CUT ENZYMES ( 78 enzymes) #
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AatII AclI AflII AflIII AgeI
AhdI Alw44I ApaLI AscI AseI
AsnI AspEI AviII AvrII BbeI
BbrPI BciVI BfrI BlnI BsaAI
BsaBI BsiEI BsiWI BspDI BspLU11I
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstZ17I ClaI CpoI EagI
Eam1105I EclXI Eco52I EcoO65I EcoRI
EcoT22I FseI FspI HindIII KasI
KspI MamI MfeI MluI MunI
NarI NheI NotI NruI NsiI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RsrII SacII SalI SexAI
SfoI SfuI SgrAI SnaBI SpeI
SspI SwaI XhoI
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# ONE CUT ENZYMES ( 30 enzymes) #
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AccII 4315 CGCG CG^CG
AccIII 1163 TCCGGA T^CCGGA
Aor51HI 122 AGCGCT AGC^GCT
BamHI 1743 GGATCC G^GATCC
BcgI 3359 GCAACCTCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BseAI 1163 TCCGGA T^CCGGA
BsmBI 3336 GAGACG CGTCTC(1/5)
BsmI 2256 GCATTC GAATGC(1/-1)
BspEI 1163 TCCGGA T^CCGGA
BspHI 4817 TCATGA T^CATGA
BsrDI 3897 CATTGC GCAATG(2/0)
BsrFI 104 GCCGGC [GA]^CCGG[CT]
BstUI 4315 CGCG CG^CG
Cfr10I 104 GCCGGC [GA]^CCGG[CT]
DraIII 1135 CACGCTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 3490 GACAGCGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 122 AGCGCT AGC^GCT
EcoRV 4123 GATATC GAT^ATC
HpaI 2461 GTTAAC GTT^AAC
MroI 1163 TCCGGA T^CCGGA
MslI 3840 CATCACCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 4315 CGCG CG^CG
NaeI 104 GCCGGC GCC^GGC
NdeI 3290 CATATG CA^TATG
NgoMIV 104 GCCGGC G^CCGGC
RcaI 4817 TCATGA T^CATGA
ScaI 3800 AGTACT AGT^ACT
SfiI 1356 GGCCCTGGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SphI 2532 GCATGC GCATG^C
XbaI 888 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 20 enzymes) #
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AccI 415 GTCTAC GT^[AC][GT]AC
739 GTCTAC
AocI 1207 CCTGAGG CC^T[ACGT]AGG
2430 CCTGAGG
Asp700I 4654 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4821 GAAACTCTTC
BbsI 1623 GAAGAC GAAGAC(2/6)
3193 GAAGAC
BclI 1113 TGATCA T^GATCA
3906 TGATCA
BglII 251 AGATCT A^GATCT
4656 AGATCT
BpuAI 1623 GAAGAC GAAGAC(2/6)
3193 GAAGAC
BsaI 373 GAGACC GGTCTC(1/5)
2745 GGTCTC
BsgI 2440 CTGCAC GTGCAG(16/14)
1141 GTGCAG
BsrBI 4266 CCGCTC CCGCTC(-3/-3)
1885 GAGCGG
BstAPI 616 GCAGTCCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
778 GCAGCCAATGC
Bsu36I 1207 CCTGAGG CC^T[ACGT]AGG
2430 CCTGAGG
DraI 2359 TTTAAA TTT^AAA
2823 TTTAAA
Eco81I 1207 CCTGAGG CC^T[ACGT]AGG
2430 CCTGAGG
FbaI 1113 TGATCA T^GATCA
3906 TGATCA
NspI 401 ACATGC [GA]CATG^[CT]
2532 GCATGC
SapI 703 GCTCTTC GCTCTTC(1/4)
1308 GCTCTTC
SbfI 1518 CCTGCAGG CCTGCA^GG
3153 CCTGCAGG
Sse8387I 1518 CCTGCAGG CCTGCA^GG
3153 CCTGCAGG
XmnI 4654 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4821 GAAACTCTTC
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# 3 OR MORE CUT ENZYMES ( 130 enzymes) #
#########################################
Acc65I AciI AcsI AcyI AfaI
AluI AlwI AlwNI ApaI ApoI
Asp718I AspHI AspI AvaI AvaII
BalI BanI BanII BbvCI BbvI
BcnI BfaI BglI BlpI BmyI
BpmI Bpu1102I BsaHI BsaJI BsaWI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I Bsp1407I BspMI
BsrGI BsrI BssKI BstF5I BstNI
BstXI BstYI Cac8I CelII CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HinfI HpaII
HphI ItaI KpnI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MspA1I
MspI MvaI MwoI NciI NcoI
NdeII NlaIII NlaIV PflFI PflMI
PleI PpuMI PstI PvuII RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SmaI SmlI SspBI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI Tth111I TthHB8I
Van91I XcmI XhoII XmaCI XmaI