Restriction map for
pFN21AB0445
##################################
# NO CUT ENZYMES ( 114 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AgeI AhdI AocI
Aor51HI AscI AseI AsnI Asp718I
AspEI AspI AviII AvrII BamHI
BbeI BbrPI BcgI BciVI BclI
BfrI BglII BlnI BsaBI BsaHI
BsaWI BseAI BsiEI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstZ17I
Bsu36I ClaI CpoI DraI DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoO65I EcoRV EcoT22I FbaI
FseI FspI HgaI Hin1I HincII
HindII HpaI KasI KpnI MamI
MfeI MluI MroI MunI NarI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SalI
SapI ScaI SfoI SfuI SgrAI
SnaBI SpeI SphI SspBI SspI
SwaI Tth111I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
AcsI 652 GAATTC [GA]^AATT[CT]
Alw44I 1316 GTGCAC G^TGCAC
ApaLI 1316 GTGCAC G^TGCAC
ApoI 652 GAATTC [GA]^AATT[CT]
Asp700I 1380 GAAAGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BanI 786 GGTGCC G^G[CT][GA]CC
BbsI 673 GAAGAC GAAGAC(2/6)
BbvCI 1050 CCTCAGC CCTCAGC(-5/-2)
BpuAI 673 GAAGAC GAAGAC(2/6)
BsaAI 813 CACGTA [CT]AC^GT[GA]
BsaI 215 GAGACC GGTCTC(1/5)
BsrDI 1194 CATTGC GCAATG(2/0)
BsrFI 95 GCCGGC [GA]^CCGG[CT]
BstXI 162 CCAGGAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 95 GCCGGC [GA]^CCGG[CT]
DraIII 362 CACTAGGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoNI 450 CCTCTGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 652 GAATTC G^AATTC
HindIII 1402 AAGCTT A^AGCTT
MaeIII 324 GTCAC ^GT[ACGT]AC
MslI 1194 CATTGCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 95 GCCGGC GCC^GGC
NgoMIV 95 GCCGGC G^CCGGC
NspI 1208 GCATGT [GA]CATG^[CT]
PpuMI 458 AGGACCT [GA]G^G[AT]CC[CT]
SbfI 1065 CCTGCAGG CCTGCA^GG
SexAI 1037 ACCTGGT A^CC[AT]GGT
SfiI 252 GGCCATGATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
Sse8387I 1065 CCTGCAGG CCTGCA^GG
StuI 1272 AGGCCT AGG^CCT
TaqI 1259 TCGA T^CGA
Tsp45I 324 GTCAC ^GT[GC]AC
Tsp509I 653 AATT ^AATT
TthHB8I 1259 TCGA T^CGA
XmnI 1380 GAAAGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AflIII 380 ACACGT A^C[GA][CT]GT
926 ACGTGT
AlwI 852 GATCC GGATC(4/5)
1100 GGATC
ApaI 421 GGGCCC GGGCC^C
465 GGGCCC
BfaI 107 CTAG C^TAG
364 CTAG
BstYI 851 AGATCC [GA]^GATC[CT]
1100 GGATCT
DpnII 852 GATC ^GATC
1101 GATC
KspI 1321 CCGCGG CCGC^GG
1392 CCGCGG
MaeI 107 CTAG C^TAG
364 CTAG
MboI 852 GATC ^GATC
1101 GATC
MflI 851 AGATCC [GA]^GATC[CT]
1100 GGATCT
MseI 875 TTAA T^TAA
1420 TTAA
NcoI 80 CCATGG C^CATGG
1436 CCATGG
NdeII 852 GATC ^GATC
1101 GATC
PvuII 30 CAGCTG CAG^CTG
1057 CAGCTG
SacI 1153 GAGCTC GAGCT^C
1327 GAGCTC
SacII 1321 CCGCGG CCGC^GG
1392 CCGCGG
Sau3AI 852 GATC ^GATC
1101 GATC
SfaNI 801 GATGC GCATC(5/9)
1137 GCATC
SmaI 248 CCCGGG CCC^GGG
1350 CCCGGG
SmlI 148 CTCAAG C^T[CT][GA]AG
1330 CTCAAG
TfiI 645 GATTC G^A[AT]TC
1345 GATTC
Tru9I 875 TTAA T^TAA
1420 TTAA
TspRI 267 CACTG CA[GC]TG[ACGT][ACGT]^
1314 CAGTG
XhoII 851 AGATCC [GA]^GATC[CT]
1100 GGATCT
XmaCI 248 CCCGGG C^CCGGG
1350 CCCGGG
XmaI 248 CCCGGG C^CCGGG
1350 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 83 enzymes) #
#########################################
AccII AciI AfaI AluI AlwNI
AspHI AvaI AvaII BalI BanII
BbvI BcnI BglI BlpI BmyI
BpmI Bpu1102I BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrI BssKI
BstAPI BstF5I BstNI BstUI Cac8I
CelII CfoI Cfr13I DdeI DraII
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeII
MboII MluNI MnlI MscI MspA1I
MspI MvaI MvnI MwoI NciI
NlaIII NlaIV PflMI PleI PstI
RsaI Sau96I ScrFI SfcI StyI
TseI Van91I XcmI