Restriction map for
pFN21AE3514
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# NO CUT ENZYMES ( 150 enzymes) #
##################################
AatII Acc65I AccI AcsI AcyI
AfaI AflII AflIII AgeI AhdI
Alw44I AlwI AlwNI AocI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvaI
AviII AvrII BalI BamHI BbeI
BbrPI BcgI BciVI BclI BfrI
BglII BlnI BsaAI BsaBI BsaHI
BsaI BsiWI BsmAI BsmBI BsmI
BsoBI Bsp1407I BspDI BspHI BspLU11I
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstXI BstYI BstZ17I
Bsu36I ClaI CpoI DraI DraIII
DrdI Eam1105I Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI Hin1I HincII HindII HindIII
HpaI KasI KpnI KspI MamI
MfeI MflI MluI MluNI MscI
MseI MslI MspA1I MunI NaeI
NarI NdeI NgoMIV NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI PstI PvuI PvuII
RcaI RsaI RsrII SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI TaqI TfiI Tru9I
Tsp509I Tth111I TthHB8I Van91I XbaI
XhoI XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 59 enzymes) #
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AccII 368 CGCG CG^CG
AccIII 215 TCCGGA T^CCGGA
AclI 685 AACGTT AA^CGTT
Aor51HI 419 AGCGCT AGC^GCT
BbsI 625 GAAGAC GAAGAC(2/6)
BbvCI 694 GCTGAGG CCTCAGC(-5/-2)
BfaI 142 CTAG C^TAG
BglI 425 GCCCCCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 606 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 606 GCTCAGC GC^T[ACGT]AGC
BpuAI 625 GAAGAC GAAGAC(2/6)
BseAI 215 TCCGGA T^CCGGA
BseRI 658 GAGGAG GAGGAG(10/8)
BsiEI 455 CGGCCG CG[GA][CT]^CG
BspEI 215 TCCGGA T^CCGGA
BspMI 360 GCAGGT ACCTGC(4/8)
BsrBI 632 CCGCTC CCGCTC(-3/-3)
BsrDI 397 CATTGC GCAATG(2/0)
BsrFI 389 ACCGGC [GA]^CCGG[CT]
BsrI 578 CCAGT ACTGG(1/-1)
BstAPI 119 GCAAAAAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 129 CATCC GGATG(2/0)
BstUI 368 CGCG CG^CG
CelII 606 GCTCAGC GC^T[ACGT]AGC
Cfr10I 389 ACCGGC [GA]^CCGG[CT]
DpnII 16 GATC ^GATC
DsaI 69 CCATGG C^C[GA][CT]GG
EagI 455 CGGCCG C^GGCCG
EarI 25 CTCTTC CTCTTC(1/4)
EclXI 455 CGGCCG C^GGCCG
Eco47III 419 AGCGCT AGC^GCT
Eco52I 455 CGGCCG C^GGCCG
EcoT14I 69 CCATGG C^C[AT][AT]GG
FokI 129 CATCC GGATG(9/13)
HgaI 259 GACGC GACGC(5/10)
HinfI 310 GACTC G^A[ACGT]TC
HphI 180 GGTGA GGTGA(8/7)
Ksp632I 25 CTCTTC CTCTTC(1/4)
MaeI 142 CTAG C^TAG
MaeII 686 ACGT A^CGT
MaeIII 274 GTGAC ^GT[ACGT]AC
MboI 16 GATC ^GATC
MroI 215 TCCGGA T^CCGGA
MvnI 368 CGCG CG^CG
NcoI 69 CCATGG C^CATGG
NdeII 16 GATC ^GATC
NheI 141 GCTAGC G^CTAGC
NspI 277 ACATGC [GA]CATG^[CT]
PleI 310 GACTC GAGTC(4/5)
Psp1406I 685 AACGTT AA^CGTT
SacI 604 GAGCTC GAGCT^C
Sau3AI 16 GATC ^GATC
SfaNI 128 GCATC GCATC(5/9)
SfcI 168 CTGTAG C^T[GA][CT]AG
SmlI 79 CTCAAG C^T[CT][GA]AG
StyI 69 CCATGG C^C[AT][AT]GG
Tsp45I 274 GTGAC ^GT[GC]AC
TspRI 579 CAGTG CA[GC]TG[ACGT][ACGT]^
XcmI 224 CCATTGGCCGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 12 enzymes) #
###################################
BanII 604 GAGCTC G[GA]GC[CT]^C
709 GAGCCC
BcnI 453 CCCGG CC^[GC]GG
483 CCGGG
BsaWI 134 TCCGGT [AT]^CCGG[AT]
215 TCCGGA
BsgI 351 CTGCAC GTGCAG(16/14)
490 CTGCAC
DdeI 607 CTCAG C^T[ACGT]AG
695 CTGAG
DraII 373 GGGACCC [GA]G^G[ACGT]CC[CT]
409 GGGTCCC
EaeI 228 TGGCCG [CT]^GGCC[GA]
455 CGGCCG
EcoO109I 373 GGGACCC [GA]G^G[ACGT]CC[CT]
409 GGGTCCC
FauI 8 GCGGG CCCGC(4/6)
437 GCGGG
HaeII 419 AGCGCT [GA]GCGC^[CT]
518 AGCGCC
NciI 453 CCCGG CC^[GC]GG
483 CCGGG
PpuMI 373 GGGACCC [GA]G^G[AT]CC[CT]
409 GGGTCCC
#########################################
# 3 OR MORE CUT ENZYMES ( 37 enzymes) #
#########################################
AciI AluI AspHI AvaII BanI
BbvI BmyI BpmI BsaJI BsiHKAI
BsiYI BslI BsmFI Bsp1286I BssKI
BstNI Cac8I CfoI Cfr13I EcoRII
Fnu4HI HaeIII HapII HhaI HinP1I
HpaII ItaI MboII MnlI MspI
MvaI MwoI NlaIII NlaIV Sau96I
ScrFI TseI