Restriction map for
pFN21AE3773
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# NO CUT ENZYMES ( 86 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AgeI Aor51HI ApaI AscI AseI
AsnI Asp718I AviII AvrII BbeI
BbrPI BcgI BlnI BsaAI BsaBI
BseAI BsiEI BsiWI BsmBI BspDI
BspEI BsrFI BssHII BssSI Bst1107I
BstUI BstZ17I Cfr10I ClaI CpoI
EagI EclXI Eco47III Eco52I EcoNI
EcoRI EcoRV FseI FspI HaeII
KasI KpnI KspI MamI MluI
MroI MvnI NaeI NarI NcoI
NgoMIV NheI NotI NruI PacI
PaeR7I PflMI PinAI PmaCI PmeI
PmlI PshBI Psp1406I PvuI RsrII
SacII SbfI ScaI SexAI SfiI
SfoI SgrAI SmaI SnaBI Sse8387I
SwaI Van91I XbaI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AcyI 1464 GACGCC G[GA]^CG[CT]C
AflII 747 CTTAAG C^TTAAG
AhdI 1696 GACAATTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 2786 GTGCAC G^TGCAC
AocI 1375 CCTGAGG CC^T[ACGT]AGG
ApaLI 2786 GTGCAC G^TGCAC
AspEI 1696 GACAATTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 3394 CCCGAG C^[CT]CG[GA]G
BanI 2910 GGCACC G^G[CT][GA]CC
BfrI 747 CTTAAG C^TTAAG
BglI 3031 GCCAGTCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 4627 AGATCT A^GATCT
BsaHI 1464 GACGCC G[GA]^CG[CT]C
BsaWI 1073 ACCGGA [AT]^CCGG[AT]
BsoBI 3394 CCCGAG C^[CT]CG[GA]G
BspHI 731 TCATGA T^CATGA
BsrBI 1589 CCGCTC CCGCTC(-3/-3)
BstEII 2310 GGTCACC G^GT[ACGT]ACC
BstPI 2310 GGTCACC G^GT[ACGT]ACC
Bsu36I 1375 CCTGAGG CC^T[ACGT]AGG
DraIII 4703 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 3792 GACACGGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 4959 CCGTGG C^C[GA][CT]GG
Eam1105I 1696 GACAATTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1375 CCTGAGG CC^T[ACGT]AGG
EcoO65I 2310 GGTCACC G^GT[ACGT]ACC
EcoT14I 1968 CCTTGG C^C[AT][AT]GG
EcoT22I 1205 ATGCAT ATGCA^T
HgaI 1464 GACGC GACGC(5/10)
Hin1I 1464 GACGCC G[GA]^CG[CT]C
HpaI 546 GTTAAC GTT^AAC
MfeI 3767 CAATTG C^AATTG
MunI 3767 CAATTG C^AATTG
NsiI 1205 ATGCAT ATGCA^T
PshAI 3471 GACAGAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 731 TCATGA T^CATGA
SacI 4540 GAGCTC GAGCT^C
SalI 4619 GTCGAC G^TCGAC
SphI 4202 GCATGC GCATG^C
StuI 3689 AGGCCT AGG^CCT
StyI 1968 CCTTGG C^C[AT][AT]GG
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# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AccI 4619 GTCGAC GT^[AC][GT]AC
4911 GTCTAC
AlwNI 1712 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
3489 CAGTCACTG
AspI 2523 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2649 GACTCAGTC
BalI 2 TGGCCA TGG^CCA
1022 TGGCCA
BamHI 18 GGATCC G^GATCC
3681 GGATCC
BanII 1785 GGGCTC G[GA]GC[CT]^C
4540 GAGCTC
BbsI 1297 GAAGAC GAAGAC(2/6)
1476 GAAGAC
BclI 1629 TGATCA T^GATCA
3111 TGATCA
BcnI 819 CCGGG CC^[GC]GG
1458 CCGGG
BlpI 556 GCTGAGC GC^T[ACGT]AGC
1591 GCTCAGC
Bpu1102I 556 GCTGAGC GC^T[ACGT]AGC
1591 GCTCAGC
BpuAI 1297 GAAGAC GAAGAC(2/6)
1476 GAAGAC
BsaI 1454 GAGACC GGTCTC(1/5)
598 GGTCTC
Bsp1407I 1959 TGTACA T^GTACA
3516 TGTACA
BspMI 4011 ACCTGC ACCTGC(4/8)
4605 ACCTGC
BsrGI 1959 TGTACA T^GTACA
3516 TGTACA
BstBI 2096 TTCGAA TT^CGAA
4646 TTCGAA
CelII 556 GCTGAGC GC^T[ACGT]AGC
1591 GCTCAGC
CfoI 2341 GCGC GCG^C
4127 GCGC
FbaI 1629 TGATCA T^GATCA
3111 TGATCA
HhaI 2341 GCGC GCG^C
4127 GCGC
HinP1I 2341 GCGC G^CGC
4127 GCGC
MluNI 2 TGGCCA TGG^CCA
1022 TGGCCA
MscI 2 TGGCCA TGG^CCA
1022 TGGCCA
NciI 819 CCGGG CC^[GC]GG
1458 CCGGG
NdeI 1388 CATATG CA^TATG
5027 CATATG
NspV 2096 TTCGAA TT^CGAA
4646 TTCGAA
PflFI 2523 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2649 GACTCAGTC
SfuI 2096 TTCGAA TT^CGAA
4646 TTCGAA
SmlI 747 CTTAAG C^T[CT][GA]AG
3170 CTCAAG
SpeI 1084 ACTAGT A^CTAGT
1857 ACTAGT
SspBI 1959 TGTACA T^GTACA
3516 TGTACA
SspI 256 AATATT AAT^ATT
838 AATATT
Tth111I 2523 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2649 GACTCAGTC
XcmI 2599 CCAGTTGGTCGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3266 CCACTACTGTCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 96 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI ApoI Asp700I AspHI AvaII
BbvCI BbvI BciVI BfaI BmyI
BpmI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
Bsp1286I BspLU11I BsrDI BsrI BssKI
BstAPI BstF5I BstNI BstXI BstYI
Cac8I Cfr13I DdeI DpnII DraI
DraII EaeI EarI EcoO109I EcoRII
FauI Fnu4HI FokI HaeIII HapII
HincII HindII HindIII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MwoI NdeII NlaIII NlaIV NspI
PleI PpuMI PstI PvuII RsaI
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII
XmnI