Restriction map for
pFN21AB0944
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# NO CUT ENZYMES ( 121 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI Alw44I AocI
Aor51HI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AvrII BbrPI
BbsI BbvCI BcgI BciVI BclI
BfaI BfrI BlnI BlpI Bpu1102I
BpuAI BsaAI BsaBI BseAI BsiWI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrDI BsrGI Bst1107I
BstBI BstEII BstPI BstZ17I Bsu36I
CelII ClaI CpoI DraI DraIII
EarI Eco47III Eco81I EcoO65I EcoRI
EcoRV EcoT22I FbaI HincII HindII
HindIII HpaI HphI KpnI Ksp632I
MaeI MaeIII MamI MfeI MroI
MseI MslI MunI NcoI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI PvuII RcaI RsrII SalI
SapI SbfI ScaI SexAI SfiI
SfuI SgrAI SmlI SnaBI SpeI
Sse8387I SspBI SspI SwaI Tru9I
Tsp45I Tsp509I Van91I XbaI XhoI
XmnI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AfaI 906 GTAC GT^AC
AflIII 791 ACGCGT A^C[GA][CT]GT
ApaI 1157 GGGCCC GGGCC^C
AspI 828 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 380 TGGCCA TGG^CCA
BglII 1146 AGATCT A^GATCT
BsaWI 226 ACCGGA [AT]^CCGG[AT]
BsrBI 262 GAGCGG CCGCTC(-3/-3)
BssHII 220 GCGCGC G^CGCGC
BssSI 304 CACGAG CACGAG(-5/-1)
BstAPI 303 GCACGAGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 384 CATCC GGATG(2/0)
BstXI 859 CCAGTGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DrdI 828 GACGCAGTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 630 CCTCGCCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FokI 384 CATCC GGATG(9/13)
KspI 144 CCGCGG CCGC^GG
MaeII 91 ACGT A^CGT
MluI 791 ACGCGT A^CGCGT
MluNI 380 TGGCCA TGG^CCA
MscI 380 TGGCCA TGG^CCA
NspI 278 GCATGC [GA]CATG^[CT]
PflFI 828 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 469 GGGTCCC [GA]G^G[AT]CC[CT]
RsaI 906 GTAC GT^AC
SacII 144 CCGCGG CCGC^GG
SfaNI 1169 GCATC GCATC(5/9)
SmaI 1003 CCCGGG CCC^GGG
SphI 278 GCATGC GCATG^C
StuI 1107 AGGCCT AGG^CCT
TaqI 365 TCGA T^CGA
TfiI 112 GATTC G^A[AT]TC
Tth111I 828 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 365 TCGA T^CGA
XcmI 856 CCACCAGTGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 1003 CCCGGG C^CCGGG
XmaI 1003 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AhdI 85 GACTCCACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
463 GACGCGGGGTC
AspEI 85 GACTCCACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
463 GACGCGGGGTC
AspHI 950 GAGCTC G[AT]GC[AT]^C
1101 GAGCTC
AviII 275 TGCGCA TGC^GCA
281 TGCGCA
BamHI 649 GGATCC G^GATCC
717 GGATCC
BglI 26 GCCTCCTCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
187 GCCGCAGAGGC
BsgI 301 CTGCAC GTGCAG(16/14)
1025 GTGCAG
BsiHKAI 950 GAGCTC G[AT]GC[AT]^C
1101 GAGCTC
BsmBI 794 CGTCTC CGTCTC(1/5)
367 GAGACG
DraII 469 GGGTCCC [GA]G^G[ACGT]CC[CT]
1156 GGGGCCC
DsaI 144 CCGCGG C^C[GA][CT]GG
501 CCGTGG
EagI 341 CGGCCG C^GGCCG
407 CGGCCG
Eam1105I 85 GACTCCACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
463 GACGCGGGGTC
EclXI 341 CGGCCG C^GGCCG
407 CGGCCG
Eco52I 341 CGGCCG C^GGCCG
407 CGGCCG
EcoO109I 469 GGGTCCC [GA]G^G[ACGT]CC[CT]
1156 GGGGCCC
FseI 72 GGCCGGCC GGCCGG^CC
342 GGCCGGCC
FspI 275 TGCGCA TGC^GCA
281 TGCGCA
NlaIII 279 CATG CATG^
977 CATG
SacI 950 GAGCTC GAGCT^C
1101 GAGCTC
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# 3 OR MORE CUT ENZYMES ( 80 enzymes) #
#########################################
AccII AciI AcyI AluI AlwI
AlwNI AvaI AvaII BanI BanII
BbeI BbvI BcnI BmyI BpmI
BsaHI BsaI BsaJI BseRI BsiEI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BsrFI BsrI BssKI BstNI
BstUI BstYI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII EaeI EcoRII
EcoT14I FauI Fnu4HI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII ItaI KasI MboI
MboII MflI MnlI MspA1I MspI
MvaI MvnI MwoI NaeI NarI
NciI NdeII NgoMIV NlaIV PleI
PstI Sau3AI Sau96I ScrFI SfcI
SfoI StyI TseI TspRI XhoII