Restriction map for
pFN21AE4303
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# NO CUT ENZYMES ( 161 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AspI AviII AvrII
BamHI BanI BanII BbeI BbrPI
BbvCI BciVI BclI BcnI BfrI
BglI BglII BlnI BlpI BmyI
BpmI Bpu1102I BsaAI BsaHI BsaI
BsaWI BseAI BseRI BsgI BsiEI
BsiHKAI BsiWI BsmBI BsmFI Bsp1286I
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrFI BsrGI BssHII
BssSI Bst1107I BstEII BstPI BstXI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoT22I
FbaI FseI FspI HaeII HapII
Hin1I HincII HindII HindIII HpaI
HpaII KasI KpnI Ksp632I KspI
MfeI MluI MroI MslI MspI
MunI NaeI NarI NciI NdeI
NgoMIV NheI NotI NruI NsiI
NspI PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PpuMI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SgrAI SmaI
SmlI SnaBI SphI Sse8387I SspBI
SspI StuI SwaI TspRI Tth111I
XbaI XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AfaI 830 GTAC GT^AC
AlwI 214 GGATC GGATC(4/5)
AvaI 170 CCCGAG C^[CT]CG[GA]G
AvaII 38 GGACC G^G[AT]CC
BalI 701 TGGCCA TGG^CCA
BbsI 256 GAAGAC GAAGAC(2/6)
BcgI 657 CGATATTGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 740 CTAG C^TAG
BpuAI 256 GAAGAC GAAGAC(2/6)
BsaBI 209 GATTGGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaJI 767 CCATGG C^C[ACGT][ACGT]GG
BsmAI 99 GAGAC GTCTC(1/5)
BsoBI 170 CCCGAG C^[CT]CG[GA]G
BsrI 441 CCAGT ACTGG(1/-1)
BstAPI 160 GCAAAATATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 65 TTCGAA TT^CGAA
BstYI 214 GGATCT [GA]^GATC[CT]
Cac8I 778 GCCAGC GC[ACGT]^[ACGT]GC
CfoI 11 GCGC GCG^C
DraII 249 GGGGCCT [GA]G^G[ACGT]CC[CT]
DsaI 767 CCATGG C^C[GA][CT]GG
EaeI 701 TGGCCA [CT]^GGCC[GA]
EcoO109I 249 GGGGCCT [GA]G^G[ACGT]CC[CT]
EcoT14I 767 CCATGG C^C[AT][AT]GG
HgaI 496 GCGTC GACGC(5/10)
HhaI 11 GCGC GCG^C
HinP1I 11 GCGC G^CGC
MaeI 740 CTAG C^TAG
MaeII 671 ACGT A^CGT
MaeIII 401 GTGAC ^GT[ACGT]AC
MamI 209 GATTGGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 214 GGATCT [GA]^GATC[CT]
MluNI 701 TGGCCA TGG^CCA
MscI 701 TGGCCA TGG^CCA
MspA1I 368 CAGCTG C[AC]G^C[GT]G
NcoI 767 CCATGG C^CATGG
NspV 65 TTCGAA TT^CGAA
PflMI 441 CCAGTTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 101 GACTAAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 881 CTGCAG CTGCA^G
PvuII 368 CAGCTG CAG^CTG
RsaI 830 GTAC GT^AC
SfcI 881 CTGCAG C^T[GA][CT]AG
SfuI 65 TTCGAA TT^CGAA
SpeI 739 ACTAGT A^CTAGT
StyI 767 CCATGG C^C[AT][AT]GG
TfiI 57 GATTC G^A[AT]TC
Tsp45I 401 GTGAC ^GT[GC]AC
Van91I 441 CCAGTTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 214 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 25 enzymes) #
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AccII 10 CGCG CG^CG
12 CGCG
AlwNI 365 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
442 CAGTTTCTG
BsiYI 441 CCAGTTTCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
481 CCCACTTTTGG
BslI 441 CCAGTTTCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
481 CCCACTTTTGG
BsmI 786 GAATGC GAATGC(1/-1)
454 GCATTC
BstF5I 120 CATCC GGATG(2/0)
758 GGATG
BstUI 10 CGCG CG^CG
12 CGCG
Cfr13I 38 GGACC G^G[ACGT]CC
250 GGGCC
DpnII 215 GATC ^GATC
295 GATC
EcoRV 88 GATATC GAT^ATC
241 GATATC
FauI 833 CCCGC CCCGC(4/6)
13 GCGGG
FokI 120 CATCC GGATG(9/13)
758 GGATG
HphI 400 GGTGA GGTGA(8/7)
92 TCACC
MboI 215 GATC ^GATC
295 GATC
MseI 560 TTAA T^TAA
617 TTAA
MvnI 10 CGCG CG^CG
12 CGCG
MwoI 13 GCGGGTGTCGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
160 GCAAAATATGC
NdeII 215 GATC ^GATC
295 GATC
NlaIII 642 CATG CATG^
768 CATG
NlaIV 249 GGGGCC GG[ACGT]^[ACGT]CC
547 GGCTCC
PleI 429 GACTC GAGTC(4/5)
730 GAGTC
Sau3AI 215 GATC ^GATC
295 GATC
Sau96I 38 GGACC G^G[ACGT]CC
250 GGGCC
SfaNI 493 GATGC GCATC(5/9)
664 GATGC
Tru9I 560 TTAA T^TAA
617 TTAA
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# 3 OR MORE CUT ENZYMES ( 22 enzymes) #
#########################################
AciI AcsI AluI ApoI BbvI
BsrDI BssKI BstNI DdeI EcoRII
Fnu4HI HaeIII HinfI ItaI MboII
MnlI MvaI ScrFI TaqI TseI
Tsp509I TthHB8I