Restriction map for
pFN21AE4260
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# NO CUT ENZYMES ( 117 enzymes) #
##################################
AatII Acc65I AccI AccIII AflII
AgeI AhdI Alw44I Aor51HI ApaI
ApaLI AscI AseI AsnI Asp718I
AspEI AspI AvaI AviII AvrII
BalI BamHI BbeI BbrPI BclI
BfrI BglI BlnI BsaI BsaWI
BseAI BsgI BsiEI BsiWI BsmI
BsoBI BspEI BspMI BsrBI BsrFI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstZ17I Cfr10I CpoI
DraIII DrdI EaeI EagI Eam1105I
EclXI Eco47III Eco52I EcoO65I EcoRI
EcoRV FbaI FseI FspI HaeII
HpaI KasI KpnI KspI MfeI
MluI MluNI MroI MscI MunI
NaeI NarI NgoMIV NotI NruI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI PvuI RsrII SacI SacII
SalI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI Sse8387I SspI StuI SwaI
Tth111I Van91I XbaI XcmI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AcyI 209 GACGCC G[GA]^CG[CT]C
AflIII 2668 ACATGT A^C[GA][CT]GT
AocI 2281 CCTGAGG CC^T[ACGT]AGG
AspHI 1838 GAGCAC G[AT]GC[AT]^C
BbvCI 1384 GCTGAGG CCTCAGC(-5/-2)
BlpI 1120 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1120 GCTGAGC GC^T[ACGT]AGC
BsaAI 118 TACGTG [CT]AC^GT[GA]
BsaBI 1210 GATTCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 209 GACGCC G[GA]^CG[CT]C
BseRI 3152 GAGGAG GAGGAG(10/8)
BsiHKAI 1838 GAGCAC G[AT]GC[AT]^C
BsmBI 1737 GAGACG CGTCTC(1/5)
Bsp1407I 918 TGTACA T^GTACA
BspDI 371 ATCGAT AT^CGAT
BspHI 753 TCATGA T^CATGA
BspLU11I 2668 ACATGT A^CATGT
BsrDI 80 GCAATG GCAATG(2/0)
BsrGI 918 TGTACA T^GTACA
Bsu36I 2281 CCTGAGG CC^T[ACGT]AGG
CelII 1120 GCTGAGC GC^T[ACGT]AGC
ClaI 371 ATCGAT AT^CGAT
DraI 1440 TTTAAA TTT^AAA
DsaI 2535 CCATGG C^C[GA][CT]GG
Eco81I 2281 CCTGAGG CC^T[ACGT]AGG
EcoNI 219 CCTCATCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 2036 ATGCAT ATGCA^T
HgaI 209 GACGC GACGC(5/10)
Hin1I 209 GACGCC G[GA]^CG[CT]C
HindIII 2501 AAGCTT A^AGCTT
MamI 1210 GATTCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NcoI 2535 CCATGG C^CATGG
NheI 1033 GCTAGC G^CTAGC
NsiI 2036 ATGCAT ATGCA^T
RcaI 753 TCATGA T^CATGA
SapI 2549 GAAGAGC GCTCTTC(1/4)
SspBI 918 TGTACA T^GTACA
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# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AclI 569 AACGTT AA^CGTT
1526 AACGTT
AlwI 374 GATCC GGATC(4/5)
768 GGATC
BanI 70 GGCACC G^G[CT][GA]CC
161 GGCACC
BanII 1153 GAGCCC G[GA]GC[CT]^C
2238 GGGCTC
BcgI 54 CGAGCCGTATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
167 GCAAGACCTTCG
BciVI 523 GTATCC GTATCC(6/5)
3012 GTATCC
BglII 720 AGATCT A^GATCT
2337 AGATCT
BpmI 184 CTGGAG CTGGAG(16/14)
434 CTGGAG
BsiYI 219 CCTCATCGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
2565 CCTGGAACGGG
BslI 219 CCTCATCGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
2565 CCTGGAACGGG
BstXI 554 CCAAAGGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2043 CCAGGTCTGTGG
DraII 215 GGGACCT [GA]G^G[ACGT]CC[CT]
240 GGGTCCC
EcoO109I 215 GGGACCT [GA]G^G[ACGT]CC[CT]
240 GGGTCCC
EcoT14I 2535 CCATGG C^C[AT][AT]GG
2868 CCAAGG
FauI 131 GCGGG CCCGC(4/6)
1944 GCGGG
HincII 547 GTTGAC GT[CT]^[GA]AC
1429 GTTGAC
HindII 547 GTTGAC GT[CT]^[GA]AC
1429 GTTGAC
HphI 2728 GGTGA GGTGA(8/7)
255 TCACC
PpuMI 215 GGGACCT [GA]G^G[AT]CC[CT]
240 GGGTCCC
Psp1406I 569 AACGTT AA^CGTT
1526 AACGTT
PstI 196 CTGCAG CTGCA^G
1582 CTGCAG
PvuII 607 CAGCTG CAG^CTG
1382 CAGCTG
SphI 2038 GCATGC GCATG^C
2912 GCATGC
StyI 2535 CCATGG C^C[AT][AT]GG
2868 CCAAGG
Tsp45I 2452 GTCAC ^GT[GC]AC
2962 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 79 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwNI ApoI Asp700I AvaII BbsI
BbvI BcnI BfaI BmyI BpuAI
BsaJI BsmAI BsmFI Bsp1286I BsrI
BssKI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
EarI EcoRII Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeI NdeII NlaIII
NlaIV NspI PleI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
TaqI TfiI Tru9I TseI Tsp509I
TspRI TthHB8I XhoII XmnI