Restriction map for
pFN21AE4215
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# NO CUT ENZYMES ( 110 enzymes) #
##################################
AatII AccI AclI AcyI AflII
AflIII AgeI Alw44I AlwNI AocI
Aor51HI ApaLI AscI AseI AsnI
Asp700I AspI AviII AvrII BamHI
BbeI BbrPI BbvCI BclI BfrI
BglI BglII BlnI BsaAI BsaHI
BsgI BsiEI BsiWI Bsp1407I BspDI
BspHI BspLU11I BsrBI BsrGI BssHII
Bst1107I BstAPI BstBI BstEII BstPI
BstZ17I Bsu36I ClaI CpoI DraI
DraIII DrdI EagI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoT22I
FbaI FspI HaeII Hin1I KasI
KspI MaeII MluI NarI NdeI
NheI NotI NruI NsiI NspI
NspV PacI PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
SapI SbfI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
Tth111I XbaI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AccII 1239 CGCG CG^CG
AccIII 1223 TCCGGA T^CCGGA
AcsI 1468 GAATTC [GA]^AATT[CT]
AhdI 833 GACCAATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 1684 GGATC GGATC(4/5)
ApaI 53 GGGCCC GGGCC^C
ApoI 1468 GAATTC [GA]^AATT[CT]
AspEI 833 GACCAATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BcnI 1716 CCGGG CC^[GC]GG
BsaBI 960 GATCAGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 1223 TCCGGA T^CCGGA
BspEI 1223 TCCGGA T^CCGGA
BspMI 1840 ACCTGC ACCTGC(4/8)
BsrFI 2149 GCCGGC [GA]^CCGG[CT]
BssSI 2101 CTCGTG CACGAG(-5/-1)
BstUI 1239 CGCG CG^CG
BstYI 1684 GGATCT [GA]^GATC[CT]
Cfr10I 2149 GCCGGC [GA]^CCGG[CT]
Eam1105I 833 GACCAATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 1468 GAATTC G^AATTC
EcoRV 1705 GATATC GAT^ATC
FauI 1885 CCCGC CCCGC(4/6)
FseI 2148 GGCCGGCC GGCCGG^CC
HgaI 561 GACGC GACGC(5/10)
HindIII 1655 AAGCTT A^AGCTT
HpaI 1081 GTTAAC GTT^AAC
MamI 960 GATCAGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 836 CAATTG C^AATTG
MflI 1684 GGATCT [GA]^GATC[CT]
MroI 1223 TCCGGA T^CCGGA
MunI 836 CAATTG C^AATTG
MvnI 1239 CGCG CG^CG
NaeI 2149 GCCGGC GCC^GGC
NciI 1716 CCGGG CC^[GC]GG
NgoMIV 2149 GCCGGC G^CCGGC
PaeR7I 1735 CTCGAG C^TCGAG
ScaI 1739 AGTACT AGT^ACT
SmlI 1735 CTCGAG C^T[CT][GA]AG
StuI 496 AGGCCT AGG^CCT
XhoI 1735 CTCGAG C^TCGAG
XhoII 1684 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 26 enzymes) #
###################################
Acc65I 302 GGTACC G^GTACC
1421 GGTACC
Asp718I 302 GGTACC G^GTACC
1421 GGTACC
AvaI 1615 CCCGAG C^[CT]CG[GA]G
1735 CTCGAG
BcgI 2093 GCAACCAGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2168 GCAATTTCATCG
BciVI 965 GTATCC GTATCC(6/5)
1275 GTATCC
BsaWI 341 ACCGGA [AT]^CCGG[AT]
1223 TCCGGA
BseRI 1050 CTCCTC GAGGAG(10/8)
662 GAGGAG
BsmBI 2178 CGTCTC CGTCTC(1/5)
1726 GAGACG
BsmI 577 GAATGC GAATGC(1/-1)
1776 GAATGC
BsoBI 1615 CCCGAG C^[CT]CG[GA]G
1735 CTCGAG
CfoI 1240 GCGC GCG^C
1893 GCGC
EarI 2196 CTCTTC CTCTTC(1/4)
1018 GAAGAG
HhaI 1240 GCGC GCG^C
1893 GCGC
HinP1I 1240 GCGC G^CGC
1893 GCGC
HincII 571 GTTGAC GT[CT]^[GA]AC
1081 GTTAAC
HindII 571 GTTGAC GT[CT]^[GA]AC
1081 GTTAAC
KpnI 302 GGTACC GGTAC^C
1421 GGTACC
Ksp632I 2196 CTCTTC CTCTTC(1/4)
1018 GAAGAG
PflMI 909 CCAACAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2139 CCAGTCAGTGG
PpuMI 199 GGGTCCC [GA]G^G[AT]CC[CT]
882 AGGACCC
PstI 982 CTGCAG CTGCA^G
2067 CTGCAG
TaqI 1415 TCGA T^CGA
1736 TCGA
TfiI 30 GAATC G^A[AT]TC
756 GATTC
TthHB8I 1415 TCGA T^CGA
1736 TCGA
Van91I 909 CCAACAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2139 CCAGTCAGTGG
XcmI 905 CCAACCAACAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2154 CCACTCCAACAATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 81 enzymes) #
#########################################
AciI AfaI AluI AspHI AvaII
BalI BanI BanII BbsI BbvI
BfaI BlpI BmyI BpmI Bpu1102I
BpuAI BsaI BsaJI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrDI
BsrI BssKI BstF5I BstNI BstXI
Cac8I CelII Cfr13I DdeI DpnII
DraII DsaI EaeI EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HinfI HpaII HphI ItaI MaeI
MaeIII MboI MboII MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MwoI NcoI NdeII NlaIII
NlaIV PleI PvuII RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI Tru9I TseI Tsp45I Tsp509I
TspRI