Restriction map for
pFN21AE4231
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# NO CUT ENZYMES ( 97 enzymes) #
##################################
AatII Acc65I AccI AclI AflIII
AgeI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI AscI Asp700I Asp718I
AspI AviII AvrII BanI BbeI
BbrPI BbsI BcgI BciVI BclI
BglI BlnI BlpI Bpu1102I BpuAI
BsaAI BsaI BsiWI BspDI BspLU11I
BsrBI BsrDI BssHII Bst1107I BstBI
BstZ17I Bsu36I CelII ClaI CpoI
DraIII Eco47III Eco81I EcoNI EcoRI
EcoRV EcoT22I FbaI FseI FspI
HindIII KasI KpnI MfeI MluI
MunI NarI NcoI NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI Psp1406I PstI PvuI
PvuII RsrII SalI SbfI SfiI
SfoI SfuI SgrAI SnaBI SphI
Sse8387I SspI SwaI Tth111I XbaI
XhoI XmnI
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# ONE CUT ENZYMES ( 64 enzymes) #
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AccII 30 CGCG CG^CG
AccIII 1331 TCCGGA T^CCGGA
AcyI 2319 GGCGTC G[GA]^CG[CT]C
AflII 252 CTTAAG C^TTAAG
AhdI 1425 GACAGAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 1425 GACAGAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 476 CCCGGG C^[CT]CG[GA]G
BbvCI 1231 CCTCAGC CCTCAGC(-5/-2)
BfrI 252 CTTAAG C^TTAAG
BglII 430 AGATCT A^GATCT
BsaHI 2319 GGCGTC G[GA]^CG[CT]C
BsaWI 1331 TCCGGA [AT]^CCGG[AT]
BseAI 1331 TCCGGA T^CCGGA
BsgI 1313 GTGCAG GTGCAG(16/14)
BsiEI 38 CGGCCG CG[GA][CT]^CG
BsmBI 1481 GAGACG CGTCTC(1/5)
BsmI 909 GAATGC GAATGC(1/-1)
BsoBI 476 CCCGGG C^[CT]CG[GA]G
Bsp1407I 1200 TGTACA T^GTACA
BspEI 1331 TCCGGA T^CCGGA
BspHI 1080 TCATGA T^CATGA
BspMI 930 GCAGGT ACCTGC(4/8)
BsrFI 40 GCCGGC [GA]^CCGG[CT]
BsrGI 1200 TGTACA T^GTACA
BssSI 879 CACGAG CACGAG(-5/-1)
BstAPI 205 GCAGCATTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 862 GGTAACC G^GT[ACGT]ACC
BstPI 862 GGTAACC G^GT[ACGT]ACC
BstUI 30 CGCG CG^CG
CfoI 1656 GCGC GCG^C
Cfr10I 40 GCCGGC [GA]^CCGG[CT]
DrdI 1215 GACAGTTGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 29 CCGCGG C^C[GA][CT]GG
EagI 38 CGGCCG C^GGCCG
Eam1105I 1425 GACAGAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 38 CGGCCG C^GGCCG
Eco52I 38 CGGCCG C^GGCCG
EcoO65I 862 GGTAACC G^GT[ACGT]ACC
HaeII 1655 GGCGCT [GA]GCGC^[CT]
HhaI 1656 GCGC GCG^C
Hin1I 2319 GGCGTC G[GA]^CG[CT]C
HinP1I 1656 GCGC G^CGC
HpaI 1726 GTTAAC GTT^AAC
KspI 29 CCGCGG CCGC^GG
MroI 1331 TCCGGA T^CCGGA
MvnI 30 CGCG CG^CG
NaeI 40 GCCGGC GCC^GGC
NdeI 599 CATATG CA^TATG
NgoMIV 40 GCCGGC G^CCGGC
PflMI 867 CCAGTTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI 1080 TCATGA T^CATGA
SacI 1176 GAGCTC GAGCT^C
SacII 29 CCGCGG CCGC^GG
SapI 1768 GAAGAGC GCTCTTC(1/4)
ScaI 2332 AGTACT AGT^ACT
SmaI 476 CCCGGG CCC^GGG
SpeI 955 ACTAGT A^CTAGT
SspBI 1200 TGTACA T^GTACA
StuI 1942 AGGCCT AGG^CCT
Tsp45I 916 GTCAC ^GT[GC]AC
Van91I 867 CCAGTTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 667 CCATATTTGGATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 476 CCCGGG C^CCGGG
XmaI 476 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AseI 268 ATTAAT AT^TAAT
2020 ATTAAT
AsnI 268 ATTAAT AT^TAAT
2020 ATTAAT
AspHI 1176 GAGCTC G[AT]GC[AT]^C
1803 GAGCAC
BalI 573 TGGCCA TGG^CCA
875 TGGCCA
BamHI 546 GGATCC G^GATCC
1627 GGATCC
BcnI 476 CCCGG CC^[GC]GG
477 CCGGG
BseRI 2399 CTCCTC GAGGAG(10/8)
1420 GAGGAG
BsiHKAI 1176 GAGCTC G[AT]GC[AT]^C
1803 GAGCAC
Cac8I 40 GCCGGC GC[ACGT]^[ACGT]GC
571 GCTGGC
DraI 222 TTTAAA TTT^AAA
699 TTTAAA
DraII 1227 AGGTCCT [GA]G^G[ACGT]CC[CT]
1464 AGGACCT
EarI 850 CTCTTC CTCTTC(1/4)
1768 GAAGAG
EcoO109I 1227 AGGTCCT [GA]G^G[ACGT]CC[CT]
1464 AGGACCT
EcoT14I 1651 CCAAGG C^C[AT][AT]GG
2292 CCTTGG
FauI 28 CCCGC CCCGC(4/6)
4 GCGGG
HincII 1582 GTTGAC GT[CT]^[GA]AC
1726 GTTAAC
HindII 1582 GTTGAC GT[CT]^[GA]AC
1726 GTTAAC
Ksp632I 850 CTCTTC CTCTTC(1/4)
1768 GAAGAG
MaeII 68 ACGT A^CGT
1336 ACGT
MluNI 573 TGGCCA TGG^CCA
875 TGGCCA
MscI 573 TGGCCA TGG^CCA
875 TGGCCA
MspA1I 29 CCGCGG C[AC]G^C[GT]G
45 CAGCGG
NciI 476 CCCGG CC^[GC]GG
477 CCGGG
PpuMI 1227 AGGTCCT [GA]G^G[AT]CC[CT]
1464 AGGACCT
PshBI 268 ATTAAT AT^TAAT
2020 ATTAAT
SexAI 393 ACCAGGT A^CC[AT]GGT
2358 ACCTGGT
SfcI 461 CTACAG C^T[GA][CT]AG
839 CTGTAG
StyI 1651 CCAAGG C^C[AT][AT]GG
2292 CCTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 69 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AvaII BanII BbvI BfaI
BmyI BpmI BsaBI BsaJI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrI
BssKI BstF5I BstNI BstXI BstYI
Cfr13I DdeI DpnII EaeI EcoRII
Fnu4HI FokI HaeIII HapII HgaI
HinfI HpaII HphI ItaI MaeI
MaeIII MamI MboI MboII MflI
MnlI MseI MslI MspI MvaI
MwoI NdeII NlaIII NlaIV PleI
RsaI Sau3AI Sau96I ScrFI SfaNI
SmlI TaqI TfiI Tru9I TseI
Tsp509I TspRI TthHB8I XhoII