Restriction map for
pFN21AE4135
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# NO CUT ENZYMES ( 127 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AgeI AhdI Aor51HI
ApaI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AviII AvrII
BamHI BbeI BbrPI BcgI BciVI
BclI BfrI BglI BlnI BlpI
Bpu1102I BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BsiEI BsiWI BsmBI
Bsp1407I BspDI BspEI BspMI BsrBI
BsrDI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstZ17I
CelII ClaI CpoI DraI DraII
DrdI EagI Eam1105I EarI EclXI
Eco47III Eco52I EcoO109I EcoO65I EcoT22I
FbaI FseI FspI HaeII HgaI
Hin1I HpaI KasI KpnI Ksp632I
KspI MaeII MamI MfeI MluI
MroI MunI NaeI NarI NdeI
NgoMIV NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI RsrII SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SnaBI SpeI
Sse8387I SspBI SspI SwaI Tth111I
XhoI XmnI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AccII 1129 CGCG CG^CG
AfaI 821 GTAC GT^AC
AflIII 443 ACATGT A^C[GA][CT]GT
Alw44I 787 GTGCAC G^TGCAC
ApaLI 787 GTGCAC G^TGCAC
BalI 879 TGGCCA TGG^CCA
BanI 1192 GGTGCC G^G[CT][GA]CC
BbsI 1507 GTCTTC GAAGAC(2/6)
BbvCI 409 GCTGAGG CCTCAGC(-5/-2)
BglII 1362 AGATCT A^GATCT
BpuAI 1507 GTCTTC GAAGAC(2/6)
BsgI 851 GTGCAG GTGCAG(16/14)
BsmI 472 GAATGC GAATGC(1/-1)
BspHI 176 TCATGA T^CATGA
BspLU11I 443 ACATGT A^CATGT
BsrFI 1025 ACCGGC [GA]^CCGG[CT]
BstUI 1129 CGCG CG^CG
BstYI 1362 AGATCT [GA]^GATC[CT]
CfoI 963 GCGC GCG^C
Cfr10I 1025 ACCGGC [GA]^CCGG[CT]
DraIII 1076 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 879 TGGCCA [CT]^GGCC[GA]
EcoRI 9 GAATTC G^AATTC
EcoRV 652 GATATC GAT^ATC
FauI 491 CCCGC CCCGC(4/6)
HhaI 963 GCGC GCG^C
HinP1I 963 GCGC G^CGC
HincII 991 GTTGAC GT[CT]^[GA]AC
HindII 991 GTTGAC GT[CT]^[GA]AC
MflI 1362 AGATCT [GA]^GATC[CT]
MluNI 879 TGGCCA TGG^CCA
MscI 879 TGGCCA TGG^CCA
MvnI 1129 CGCG CG^CG
NheI 70 GCTAGC G^CTAGC
RcaI 176 TCATGA T^CATGA
RsaI 821 GTAC GT^AC
SmaI 1054 CCCGGG CCC^GGG
SphI 1340 GCATGC GCATG^C
StuI 1448 AGGCCT AGG^CCT
TaqI 405 TCGA T^CGA
TfiI 79 GAATC G^A[AT]TC
TthHB8I 405 TCGA T^CGA
XbaI 189 TCTAGA T^CTAGA
XhoII 1362 AGATCT [GA]^GATC[CT]
XmaCI 1054 CCCGGG C^CCGGG
XmaI 1054 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AlwNI 364 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG
1181 CAGGGGCTG
AocI 14 CCTCAGG CC^T[ACGT]AGG
1444 CCTGAGG
AvaI 1019 CTCGGG C^[CT]CG[GA]G
1054 CCCGGG
BsoBI 1019 CTCGGG C^[CT]CG[GA]G
1054 CCCGGG
BstXI 218 CCATTTCGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1465 CCATCCCTCTGG
Bsu36I 14 CCTCAGG CC^T[ACGT]AGG
1444 CCTGAGG
Eco81I 14 CCTCAGG CC^T[ACGT]AGG
1444 CCTGAGG
EcoNI 306 CCTGAAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1440 CCTTCCTGAGG
EcoT14I 104 CCATGG C^C[AT][AT]GG
387 CCATGG
HindIII 762 AAGCTT A^AGCTT
1549 AAGCTT
MseI 719 TTAA T^TAA
1592 TTAA
MslI 219 CATTTCGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1038 CACCCTGGTG
NcoI 104 CCATGG C^CATGG
387 CCATGG
PstI 337 CTGCAG CTGCA^G
604 CTGCAG
SfcI 337 CTGCAG C^T[GA][CT]AG
604 CTGCAG
SmlI 24 CTCAAG C^T[CT][GA]AG
167 CTCAAG
StyI 104 CCATGG C^C[AT][AT]GG
387 CCATGG
Tru9I 719 TTAA T^TAA
1592 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 67 enzymes) #
#########################################
AciI AcsI AluI AlwI ApoI
AspHI AvaII BanII BbvI BcnI
BfaI BmyI BpmI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrI BssKI BstF5I BstNI
Cac8I Cfr13I DdeI DpnII DsaI
EcoRII Fnu4HI FokI HaeIII HapII
HinfI HpaII HphI ItaI MaeI
MaeIII MboI MboII MnlI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PflMI PleI
PvuII SacI Sau3AI Sau96I ScrFI
SfaNI TseI Tsp45I Tsp509I TspRI
Van91I XcmI