Restriction map for
pFN21AE2173
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# NO CUT ENZYMES ( 129 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI Asp718I AspEI AspI AviII
BalI BamHI BanI BbeI BbrPI
BciVI BfrI BglI BlpI Bpu1102I
BsaHI BsaI BsaWI BseAI BseRI
BsiEI BsiWI BsmFI Bsp1407I BspDI
BspEI BspLU11I BspMI BsrBI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstEII BstPI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DraII DraIII
DrdI DsaI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRI EcoRV FauI FseI
FspI HgaI Hin1I HindIII HpaI
KasI KpnI KspI MluI MluNI
MroI MscI MslI NaeI NarI
NcoI NgoMIV NheI NotI NruI
NspI PacI PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI Psp1406I
PvuI RsrII SacII SalI SapI
SbfI SexAI SfiI SfoI SgrAI
SmaI SpeI SphI Sse8387I SspBI
SspI SwaI Tsp45I Tth111I Van91I
XbaI XcmI XmaCI XmaI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AccII 2090 CGCG CG^CG
AspHI 453 GAGCTC G[AT]GC[AT]^C
AvaI 839 CTCGAG C^[CT]CG[GA]G
AvaII 169 GGACC G^G[AT]CC
AvrII 310 CCTAGG C^CTAGG
BbsI 1908 GTCTTC GAAGAC(2/6)
BbvCI 699 GCTGAGG CCTCAGC(-5/-2)
BcgI 1119 CGAGTTTGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 404 CCGGG CC^[GC]GG
BlnI 310 CCTAGG C^CTAGG
BpuAI 1908 GTCTTC GAAGAC(2/6)
BsaAI 180 TACGTA [CT]AC^GT[GA]
BsaBI 1642 GATCTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 1127 CTGCAC GTGCAG(16/14)
BsiHKAI 453 GAGCTC G[AT]GC[AT]^C
BsiYI 678 CCTTCGACTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 678 CCTTCGACTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmBI 1688 GAGACG CGTCTC(1/5)
BsmI 807 GAATGC GAATGC(1/-1)
BsoBI 839 CTCGAG C^[CT]CG[GA]G
BspHI 2135 TCATGA T^CATGA
BsrDI 1664 CATTGC GCAATG(2/0)
BstBI 209 TTCGAA TT^CGAA
BstUI 2090 CGCG CG^CG
EaeI 1184 TGGCCG [CT]^GGCC[GA]
EcoT14I 310 CCTAGG C^C[AT][AT]GG
EcoT22I 299 ATGCAT ATGCA^T
HaeII 1859 AGCGCC [GA]GCGC^[CT]
HapII 404 CCGG C^CGG
HincII 2148 GTTGAC GT[CT]^[GA]AC
HindII 2148 GTTGAC GT[CT]^[GA]AC
HpaII 404 CCGG C^CGG
MamI 1642 GATCTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 2036 CAATTG C^AATTG
MspI 404 CCGG C^CGG
MunI 2036 CAATTG C^AATTG
MvnI 2090 CGCG CG^CG
NciI 404 CCGGG CC^[GC]GG
NdeI 2183 CATATG CA^TATG
NsiI 299 ATGCAT ATGCA^T
NspV 209 TTCGAA TT^CGAA
PaeR7I 839 CTCGAG C^TCGAG
PshAI 438 GACACCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 2135 TCATGA T^CATGA
SacI 453 GAGCTC GAGCT^C
ScaI 946 AGTACT AGT^ACT
SfuI 209 TTCGAA TT^CGAA
SnaBI 180 TACGTA TAC^GTA
StyI 310 CCTAGG C^C[AT][AT]GG
XhoI 839 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 21 enzymes) #
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AciI 1883 CCGC CCGC(-3/-1)
1069 GCGG
AseI 1281 ATTAAT AT^TAAT
1915 ATTAAT
AsnI 1281 ATTAAT AT^TAAT
1915 ATTAAT
Asp700I 1405 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1693 GAAGAAATTC
BanII 453 GAGCTC G[GA]GC[CT]^C
851 GAGCCC
BclI 54 TGATCA T^GATCA
2040 TGATCA
BglII 36 AGATCT A^GATCT
1641 AGATCT
BmyI 453 GAGCTC G[AGT]GC[ACT]^C
851 GAGCCC
Bsp1286I 453 GAGCTC G[AGT]GC[ACT]^C
851 GAGCCC
CfoI 1860 GCGC GCG^C
2089 GCGC
Cfr13I 169 GGACC G^G[ACGT]CC
1458 GGGCC
DraI 42 TTTAAA TTT^AAA
720 TTTAAA
FbaI 54 TGATCA T^GATCA
2040 TGATCA
HhaI 1860 GCGC GCG^C
2089 GCGC
HinP1I 1860 GCGC G^CGC
2089 GCGC
MaeII 181 ACGT A^CGT
1155 ACGT
NlaIV 396 GGAGCC GG[ACGT]^[ACGT]CC
530 GGAGCC
PshBI 1281 ATTAAT AT^TAAT
1915 ATTAAT
Sau96I 169 GGACC G^G[ACGT]CC
1458 GGGCC
SmlI 764 CTCAAG C^T[CT][GA]AG
839 CTCGAG
XmnI 1405 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1693 GAAGAAATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 58 enzymes) #
#########################################
AcsI AfaI AluI AlwI AlwNI
ApoI BbvI BfaI BpmI BsaJI
BsmAI BsrI BssKI BstF5I BstNI
BstXI BstYI Cac8I DdeI DpnII
EarI EcoRII Fnu4HI FokI HaeIII
HinfI HphI ItaI Ksp632I MaeI
MaeIII MboI MboII MflI MnlI
MseI MspA1I MvaI MwoI NdeII
NlaIII PleI PstI PvuII RsaI
Sau3AI ScrFI SfaNI SfcI StuI
TaqI TfiI Tru9I TseI Tsp509I
TspRI TthHB8I XhoII