Restriction map for
pFN21AE4064
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# NO CUT ENZYMES ( 123 enzymes) #
##################################
AatII Acc65I AccI AclI AflII
AgeI AhdI AlwNI AscI AseI
AsnI Asp718I AspEI AspI AviII
AvrII BalI BamHI BbeI BbvCI
BcgI BciVI BclI BfaI BfrI
BglI BglII BlnI BlpI Bpu1102I
BsaBI BsaI BseRI BsiEI BsmI
BspDI BspHI BspLU11I BsrDI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstZ17I CelII ClaI CpoI
DraI DraIII EagI Eam1105I EarI
EclXI Eco52I EcoNI EcoO65I EcoRI
EcoT14I EcoT22I FbaI FseI FspI
HincII HindII HindIII HpaI KasI
KpnI Ksp632I KspI MaeI MamI
MfeI MluI MluNI MscI MunI
NarI NcoI NheI NotI NruI
NsiI NspI NspV PaeR7I PflFI
PinAI PleI PmeI PshAI PshBI
Psp1406I PvuI PvuII RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SfiI SfoI SfuI SgrAI
SmlI SnaBI SpeI SphI Sse8387I
SspI StuI StyI SwaI Tth111I
XbaI XcmI XhoI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AcsI 471 GAATTT [GA]^AATT[CT]
AflIII 908 ACACGT A^C[GA][CT]GT
Alw44I 1007 GTGCAC G^TGCAC
AocI 58 CCTGAGG CC^T[ACGT]AGG
ApaLI 1007 GTGCAC G^TGCAC
ApoI 471 GAATTT [GA]^AATT[CT]
Asp700I 87 GAAGTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbrPI 935 CACGTG CAC^GTG
BsaAI 935 CACGTG [CT]AC^GT[GA]
BsgI 932 CTGCAC GTGCAG(16/14)
BsiWI 41 CGTACG C^GTACG
BsmBI 661 CGTCTC CGTCTC(1/5)
Bsp1407I 410 TGTACA T^GTACA
BsrBI 52 GAGCGG CCGCTC(-3/-3)
BsrFI 636 GCCGGC [GA]^CCGG[CT]
BsrGI 410 TGTACA T^GTACA
BsrI 1023 CCAGT ACTGG(1/-1)
BstF5I 233 GGATG GGATG(2/0)
BstXI 98 CCAGCTACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 746 GGATCT [GA]^GATC[CT]
Bsu36I 58 CCTGAGG CC^T[ACGT]AGG
Cfr10I 636 GCCGGC [GA]^CCGG[CT]
DrdI 598 GACCAGGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 575 CGGCCA [CT]^GGCC[GA]
Eco81I 58 CCTGAGG CC^T[ACGT]AGG
EcoRV 485 GATATC GAT^ATC
FokI 233 GGATG GGATG(9/13)
MflI 746 GGATCT [GA]^GATC[CT]
MslI 909 CACGTTTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 636 GCCGGC GCC^GGC
NdeI 428 CATATG CA^TATG
NgoMIV 636 GCCGGC G^CCGGC
NlaIII 579 CATG CATG^
PacI 805 TTAATTAA TTAAT^TAA
PflMI 444 CCAGGGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 935 CACGTG CAC^GTG
PmlI 935 CACGTG CAC^GTG
SexAI 104 ACCTGGT A^CC[AT]GGT
SfaNI 535 GATGC GCATC(5/9)
SmaI 979 CCCGGG CCC^GGG
SspBI 410 TGTACA T^GTACA
Van91I 444 CCAGGGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 746 GGATCT [GA]^GATC[CT]
XmaCI 979 CCCGGG C^CCGGG
XmaI 979 CCCGGG C^CCGGG
XmnI 87 GAAGTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccII 20 CGCG CG^CG
226 CGCG
AccIII 194 TCCGGA T^CCGGA
374 TCCGGA
AfaI 42 GTAC GT^AC
411 GTAC
AlwI 32 GGATC GGATC(4/5)
746 GGATC
Aor51HI 14 AGCGCT AGC^GCT
185 AGCGCT
AspHI 214 GAGCAC G[AT]GC[AT]^C
1007 GTGCAC
AvaI 610 CTCGGG C^[CT]CG[GA]G
979 CCCGGG
BanI 434 GGCACC G^G[CT][GA]CC
1019 GGCACC
BbsI 378 GAAGAC GAAGAC(2/6)
591 GAAGAC
BpuAI 378 GAAGAC GAAGAC(2/6)
591 GAAGAC
BseAI 194 TCCGGA T^CCGGA
374 TCCGGA
BsiHKAI 214 GAGCAC G[AT]GC[AT]^C
1007 GTGCAC
BsmAI 733 GAGAC GTCTC(1/5)
662 GTCTC
BsoBI 610 CTCGGG C^[CT]CG[GA]G
979 CCCGGG
BspEI 194 TCCGGA T^CCGGA
374 TCCGGA
BspMI 971 ACCTGC ACCTGC(4/8)
918 GCAGGT
BstUI 20 CGCG CG^CG
226 CGCG
DsaI 383 CCACGG C^C[GA][CT]GG
728 CCGTGG
Eco47III 14 AGCGCT AGC^GCT
185 AGCGCT
HaeII 14 AGCGCT [GA]GCGC^[CT]
185 AGCGCT
HphI 116 TCACC GGTGA(8/7)
969 TCACC
MaeIII 109 GTCAC ^GT[ACGT]AC
905 GTCAC
MroI 194 TCCGGA T^CCGGA
374 TCCGGA
MspA1I 208 CCGCTG C[AC]G^C[GT]G
693 CCGCTG
MvnI 20 CGCG CG^CG
226 CGCG
PstI 148 CTGCAG CTGCA^G
973 CTGCAG
RsaI 42 GTAC GT^AC
411 GTAC
Tsp45I 109 GTCAC ^GT[GC]AC
905 GTCAC
Tsp509I 472 AATT ^AATT
807 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 60 enzymes) #
#########################################
AciI AcyI AluI ApaI AvaII
BanII BbvI BcnI BmyI BpmI
BsaHI BsaJI BsaWI BsiYI BslI
BsmFI Bsp1286I BssKI BstNI Cac8I
CfoI Cfr13I DdeI DpnII DraII
EcoO109I EcoRII FauI Fnu4HI HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII ItaI MaeII MboI
MboII MnlI MseI MspI MvaI
MwoI NciI NdeII NlaIV PpuMI
Sau3AI Sau96I ScrFI SfcI TaqI
TfiI Tru9I TseI TspRI TthHB8I