Restriction map for
pFN21AE4087
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# NO CUT ENZYMES ( 156 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaLI
AscI AseI AsnI Asp718I AspEI
AviII AvrII BalI BamHI BanI
BbeI BbrPI BbsI BcgI BciVI
BclI BfrI BglII BlnI BlpI
Bpu1102I BpuAI BsaAI BsaHI BsaI
BsaWI BseAI BsiEI BsiWI BsmBI
Bsp1407I BspDI BspEI BspLU11I BspMI
BsrBI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstBI BstUI BstXI
BstYI BstZ17I Bsu36I CelII CfoI
Cfr10I ClaI CpoI DraI DraIII
DrdI DsaI EaeI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoNI EcoT14I EcoT22I FbaI FseI
FspI HaeII HgaI HhaI Hin1I
HinP1I HpaI KasI KpnI Ksp632I
KspI MaeII MfeI MflI MluI
MluNI MroI MscI MspA1I MunI
MvnI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI PvuII RsrII
SacII SalI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI StyI SwaI
XbaI XcmI XhoI XhoII XmaCI
XmaI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AciI 560 CCGC CCGC(-3/-1)
AfaI 952 GTAC GT^AC
AlwI 366 GGATC GGATC(4/5)
ApaI 817 GGGCCC GGGCC^C
AvaI 1011 CTCGGG C^[CT]CG[GA]G
AvaII 1032 GGTCC G^G[AT]CC
BbvCI 397 GCTGAGG CCTCAGC(-5/-2)
BglI 649 GCCAAAATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 345 CTCCAG CTGGAG(16/14)
BsaBI 1270 GATGTTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaJI 1010 CCTCGG C^C[ACGT][ACGT]GG
BsmI 160 GAATGC GAATGC(1/-1)
BsoBI 1011 CTCGGG C^[CT]CG[GA]G
BspHI 876 TCATGA T^CATGA
BstAPI 988 GCATGGGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 75 GGTCACC G^GT[ACGT]ACC
BstPI 75 GGTCACC G^GT[ACGT]ACC
DpnII 367 GATC ^GATC
DraII 816 AGGGCCC [GA]G^G[ACGT]CC[CT]
EcoO109I 816 AGGGCCC [GA]G^G[ACGT]CC[CT]
EcoO65I 75 GGTCACC G^GT[ACGT]ACC
EcoRI 3 GAATTC G^AATTC
EcoRV 895 GATATC GAT^ATC
FauI 559 CCCGC CCCGC(4/6)
HincII 171 GTCAAC GT[CT]^[GA]AC
HindII 171 GTCAAC GT[CT]^[GA]AC
MamI 1270 GATGTTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboI 367 GATC ^GATC
NdeII 367 GATC ^GATC
PflMI 494 CCAGATGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI 876 TCATGA T^CATGA
RsaI 952 GTAC GT^AC
Sau3AI 367 GATC ^GATC
SspI 1019 AATATT AAT^ATT
TaqI 630 TCGA T^CGA
TthHB8I 630 TCGA T^CGA
Van91I 494 CCAGATGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 18 enzymes) #
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Asp700I 57 GAACTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
300 GAAGATGTTC
AspI 70 GACCCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
374 GACACTGTC
BcnI 72 CCCGG CC^[GC]GG
363 CCGGG
BsgI 778 CTGCAC GTGCAG(16/14)
812 GTGCAG
HapII 73 CCGG C^CGG
363 CCGG
HindIII 415 AAGCTT A^AGCTT
449 AAGCTT
HpaII 73 CCGG C^CGG
363 CCGG
MaeIII 76 GTCAC ^GT[ACGT]AC
938 GTGAC
MspI 73 CCGG C^CGG
363 CCGG
NciI 72 CCCGG CC^[GC]GG
363 CCGGG
NlaIV 323 GGAGCC GG[ACGT]^[ACGT]CC
817 GGGCCC
NspI 782 ACATGC [GA]CATG^[CT]
923 GCATGT
PflFI 70 GACCCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
374 GACACTGTC
SacI 258 GAGCTC GAGCT^C
805 GAGCTC
StuI 106 AGGCCT AGG^CCT
1330 AGGCCT
Tsp45I 76 GTCAC ^GT[GC]AC
938 GTGAC
Tth111I 70 GACCCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
374 GACACTGTC
XmnI 57 GAACTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
300 GAAGATGTTC
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# 3 OR MORE CUT ENZYMES ( 47 enzymes) #
#########################################
AcsI AluI AlwNI ApoI AspHI
BanII BbvI BfaI BmyI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrI BssKI BstF5I BstNI
Cac8I Cfr13I DdeI EcoRII Fnu4HI
FokI HaeIII HinfI HphI ItaI
MaeI MboII MnlI MseI MslI
MvaI MwoI NlaIII PleI Sau96I
ScrFI SfaNI TfiI Tru9I TseI
Tsp509I TspRI