Restriction map for
pFN21AE4191
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# NO CUT ENZYMES ( 99 enzymes) #
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AatII AccI AclI AflII AgeI
AhdI Alw44I Aor51HI ApaLI AscI
AseI AsnI AspEI AspI AvrII
BbeI BbrPI BcgI BclI BfrI
BglII BlnI BsaBI BsaI BsiWI
BsmI Bsp1407I BspDI BspLU11I BsrGI
BssHII Bst1107I BstBI BstEII BstPI
BstZ17I ClaI CpoI DraI DraIII
Eam1105I Eco47III EcoO65I EcoRV EcoT22I
FbaI FseI HindIII HpaI KasI
KspI MamI MluI MseI NaeI
NarI NdeI NgoMIV NheI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RsrII
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I SspBI
SspI SwaI Tru9I Tth111I Van91I
XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 53 enzymes) #
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Acc65I 716 GGTACC G^GTACC
AccIII 37 TCCGGA T^CCGGA
AcsI 844 GAATTC [GA]^AATT[CT]
AcyI 1496 GGCGTC G[GA]^CG[CT]C
ApoI 844 GAATTC [GA]^AATT[CT]
Asp700I 1842 GAACCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 716 GGTACC G^GTACC
AvaI 649 CCCGAG C^[CT]CG[GA]G
BamHI 1738 GGATCC G^GATCC
BanI 716 GGTACC G^G[CT][GA]CC
BbsI 400 GAAGAC GAAGAC(2/6)
BciVI 732 GTATCC GTATCC(6/5)
BglI 10 GCCTCCGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 549 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 549 GCTGAGC GC^T[ACGT]AGC
BpuAI 400 GAAGAC GAAGAC(2/6)
BsaAI 615 CACGTA [CT]AC^GT[GA]
BsaHI 1496 GGCGTC G[GA]^CG[CT]C
BsaWI 37 TCCGGA [AT]^CCGG[AT]
BseAI 37 TCCGGA T^CCGGA
BsgI 940 CTGCAC GTGCAG(16/14)
BsmBI 1498 CGTCTC CGTCTC(1/5)
BsoBI 649 CCCGAG C^[CT]CG[GA]G
BspEI 37 TCCGGA T^CCGGA
BspHI 1073 TCATGA T^CATGA
BsrFI 269 ACCGGC [GA]^CCGG[CT]
BssSI 628 CACGAG CACGAG(-5/-1)
BstAPI 942 GCACGACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 549 GCTGAGC GC^T[ACGT]AGC
Cfr10I 269 ACCGGC [GA]^CCGG[CT]
DrdI 1564 GACCCTCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 1115 CCATGG C^C[GA][CT]GG
EarI 1207 CTCTTC CTCTTC(1/4)
EcoRI 844 GAATTC G^AATTC
EcoT14I 1115 CCATGG C^C[AT][AT]GG
Hin1I 1496 GGCGTC G[GA]^CG[CT]C
HincII 1453 GTTGAC GT[CT]^[GA]AC
HindII 1453 GTTGAC GT[CT]^[GA]AC
KpnI 716 GGTACC GGTAC^C
Ksp632I 1207 CTCTTC CTCTTC(1/4)
MfeI 355 CAATTG C^AATTG
MroI 37 TCCGGA T^CCGGA
MunI 355 CAATTG C^AATTG
NcoI 1115 CCATGG C^CATGG
NotI 76 GCGGCCGC GC^GGCCGC
PstI 2011 CTGCAG CTGCA^G
RcaI 1073 TCATGA T^CATGA
SacI 62 GAGCTC GAGCT^C
SphI 1274 GCATGC GCATG^C
StyI 1115 CCATGG C^C[AT][AT]GG
TfiI 1672 GATTC G^A[AT]TC
XcmI 1718 CCACTGGTGATATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 1842 GAACCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AlwNI 1193 CAGGCGCTG CAG[ACGT][ACGT][ACGT]^CTG
1358 CAGACACTG
AocI 832 CCTGAGG CC^T[ACGT]AGG
913 CCTGAGG
ApaI 815 GGGCCC GGGCC^C
890 GGGCCC
AspHI 62 GAGCTC G[AT]GC[AT]^C
1882 GAGCAC
AviII 878 TGCGCA TGC^GCA
1901 TGCGCA
BsiEI 77 CGGCCG CG[GA][CT]^CG
1107 CGGCCG
BsiHKAI 62 GAGCTC G[AT]GC[AT]^C
1882 GAGCAC
BsmAI 1506 GAGAC GTCTC(1/5)
1499 GTCTC
BsrBI 153 CCGCTC CCGCTC(-3/-3)
757 GAGCGG
BsrDI 392 CATTGC GCAATG(2/0)
1904 GCAATG
Bsu36I 832 CCTGAGG CC^T[ACGT]AGG
913 CCTGAGG
EagI 77 CGGCCG C^GGCCG
1107 CGGCCG
EclXI 77 CGGCCG C^GGCCG
1107 CGGCCG
Eco52I 77 CGGCCG C^GGCCG
1107 CGGCCG
Eco81I 832 CCTGAGG CC^T[ACGT]AGG
913 CCTGAGG
FspI 878 TGCGCA TGC^GCA
1901 TGCGCA
HgaI 1497 GCGTC GACGC(5/10)
1973 GCGTC
MslI 510 CACTCAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
676 CATGGCGGTG
NspI 1274 GCATGC [GA]CATG^[CT]
1406 ACATGC
PleI 2029 GACTC GAGTC(4/5)
116 GAGTC
PvuII 1576 CAGCTG CAG^CTG
1589 CAGCTG
StuI 1414 AGGCCT AGG^CCT
1709 AGGCCT
TaqI 188 TCGA T^CGA
1259 TCGA
Tsp45I 1004 GTGAC ^GT[GC]AC
1285 GTCAC
Tsp509I 356 AATT ^AATT
845 AATT
TthHB8I 188 TCGA T^CGA
1259 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 80 enzymes) #
#########################################
AccII AciI AfaI AflIII AluI
AlwI AvaII BalI BanII BbvCI
BbvI BcnI BfaI BmyI BpmI
BsaJI BseRI BsiYI BslI BsmFI
Bsp1286I BspMI BsrI BssKI BstF5I
BstNI BstUI BstXI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraII
EaeI EcoNI EcoO109I EcoRII FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV PpuMI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI TseI TspRI XhoII