Restriction map for
pFN21AE4121
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# NO CUT ENZYMES ( 121 enzymes) #
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AatII Acc65I AccI AccIII AgeI
Alw44I AocI Aor51HI ApaLI AscI
AseI AsnI Asp718I AspI AvaII
AviII AvrII BamHI BanI BbeI
BbrPI BbvCI BcgI BciVI BclI
BcnI BglI BlnI BlpI Bpu1102I
BsaAI BsaWI BseAI BseRI BsiEI
BsiWI Bsp1407I BspDI BspEI BspMI
BsrBI BsrDI BsrGI BssHII Bst1107I
BstAPI BstBI BstZ17I Bsu36I CelII
ClaI CpoI DraI DrdI DsaI
EagI EclXI Eco47III Eco52I Eco81I
FbaI FseI FspI HaeII HincII
HindII HindIII HpaI KasI KpnI
KspI MfeI MluI MroI MunI
NarI NciI NcoI NdeI NheI
NotI NruI NspV PacI PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI PstI PvuI PvuII
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfcI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI Tsp45I Tth111I XbaI XmaCI
XmaI
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# ONE CUT ENZYMES ( 54 enzymes) #
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AccII 1058 CGCG CG^CG
AclI 1462 AACGTT AA^CGTT
AflII 49 CTTAAG C^TTAAG
AflIII 1193 ACATGT A^C[GA][CT]GT
AhdI 1150 GACGCCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 793 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 272 GGGCCC GGGCC^C
AspEI 1150 GACGCCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 584 GTGCTC G[AT]GC[AT]^C
AvaI 893 CTCGAG C^[CT]CG[GA]G
BalI 1200 TGGCCA TGG^CCA
BfaI 1147 CTAG C^TAG
BfrI 49 CTTAAG C^TTAAG
BglII 744 AGATCT A^GATCT
BsaBI 1180 GATTTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1238 GGTCTC GGTCTC(1/5)
BsgI 921 GTGCAG GTGCAG(16/14)
BsiHKAI 584 GTGCTC G[AT]GC[AT]^C
BsmBI 694 CGTCTC CGTCTC(1/5)
BsmFI 805 GTCCC GGGAC(10/14)
BsmI 539 GAATGC GAATGC(1/-1)
BsoBI 893 CTCGAG C^[CT]CG[GA]G
BspLU11I 1193 ACATGT A^CATGT
BssSI 626 CTCGTG CACGAG(-5/-1)
BstEII 333 GGTTACC G^GT[ACGT]ACC
BstPI 333 GGTTACC G^GT[ACGT]ACC
BstUI 1058 CGCG CG^CG
BstXI 1334 CCAAGAAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraII 271 GGGGCCC [GA]G^G[ACGT]CC[CT]
Eam1105I 1150 GACGCCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 1208 CCTCTCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 271 GGGGCCC [GA]G^G[ACGT]CC[CT]
EcoO65I 333 GGTTACC G^GT[ACGT]ACC
EcoRI 68 GAATTC G^AATTC
EcoRV 862 GATATC GAT^ATC
EcoT14I 267 CCAAGG C^C[AT][AT]GG
EcoT22I 541 ATGCAT ATGCA^T
MaeI 1147 CTAG C^TAG
MaeII 1463 ACGT A^CGT
MamI 1180 GATTTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 1200 TGGCCA TGG^CCA
MscI 1200 TGGCCA TGG^CCA
MslI 867 CATTGAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 1058 CGCG CG^CG
NaeI 966 GCCGGC GCC^GGC
NgoMIV 966 GCCGGC G^CCGGC
NsiI 541 ATGCAT ATGCA^T
NspI 1193 ACATGT [GA]CATG^[CT]
PaeR7I 893 CTCGAG C^TCGAG
Psp1406I 1462 AACGTT AA^CGTT
StuI 242 AGGCCT AGG^CCT
StyI 267 CCAAGG C^C[AT][AT]GG
XcmI 521 CCAAGCTGTTTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 893 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AfaI 168 GTAC GT^AC
681 GTAC
Asp700I 345 GAAGAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
348 GAAATTCTTC
BanII 272 GGGCCC G[GA]GC[CT]^C
770 GAGCCC
BspHI 686 TCATGA T^CATGA
1322 TCATGA
BsrFI 425 ACCGGC [GA]^CCGG[CT]
966 GCCGGC
CfoI 777 GCGC GCG^C
1059 GCGC
Cfr10I 425 ACCGGC [GA]^CCGG[CT]
966 GCCGGC
Cfr13I 272 GGGCC G^G[ACGT]CC
273 GGCCC
DraIII 601 CACAATGTG CAC[ACGT][ACGT][ACGT]^GTG
915 CACCAAGTG
EaeI 608 TGGCCG [CT]^GGCC[GA]
1200 TGGCCA
EarI 163 GAAGAG CTCTTC(1/4)
238 GAAGAG
FauI 326 CCCGC CCCGC(4/6)
228 GCGGG
HapII 426 CCGG C^CGG
967 CCGG
HhaI 777 GCGC GCG^C
1059 GCGC
HinP1I 777 GCGC G^CGC
1059 GCGC
HpaII 426 CCGG C^CGG
967 CCGG
Ksp632I 163 GAAGAG CTCTTC(1/4)
238 GAAGAG
MspA1I 226 CAGCGG C[AC]G^C[GT]G
974 CAGCGG
MspI 426 CCGG C^CGG
967 CCGG
NlaIV 271 GGGGCC GG[ACGT]^[ACGT]CC
272 GGGCCC
RcaI 686 TCATGA T^CATGA
1322 TCATGA
RsaI 168 GTAC GT^AC
681 GTAC
Sau96I 272 GGGCC G^G[ACGT]CC
273 GGCCC
TaqI 653 TCGA T^CGA
894 TCGA
TthHB8I 653 TCGA T^CGA
894 TCGA
XmnI 345 GAAGAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
348 GAAATTCTTC
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# 3 OR MORE CUT ENZYMES ( 57 enzymes) #
#########################################
AciI AcsI AcyI AluI AlwI
ApoI BbsI BbvI BmyI BpmI
BpuAI BsaHI BsaJI BsiYI BslI
BsmAI Bsp1286I BsrI BssKI BstF5I
BstNI BstYI Cac8I DdeI DpnII
EcoRII Fnu4HI FokI HaeIII HgaI
Hin1I HinfI HphI ItaI MaeIII
MboI MboII MflI MnlI MseI
MvaI MwoI NdeII NlaIII PflMI
PleI Sau3AI ScrFI SfaNI SmlI
TfiI Tru9I TseI Tsp509I TspRI
Van91I XhoII