Restriction map for
pFN21AE3165
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# NO CUT ENZYMES ( 141 enzymes) #
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AatII Acc65I AccIII AclI AcyI
AflII AflIII AgeI Alw44I AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp718I AspHI AspI AvaI
AviII AvrII BalI BamHI BanI
BanII BbeI BbrPI BcgI BcnI
BfrI BlnI BlpI BmyI Bpu1102I
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BsiEI BsiHKAI BsmBI BsmFI
BsoBI Bsp1286I BspDI BspEI BspLU11I
BspMI BsrBI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstZ17I
Bsu36I CelII ClaI CpoI DraI
DraII DraIII DrdI EagI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRI EcoRV EcoT22I FseI
FspI HaeII Hin1I HindIII KasI
KpnI KspI MaeII MamI MfeI
MluI MluNI MroI MscI MunI
NaeI NarI NciI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI RsrII
SacI SacII SbfI ScaI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspI StuI
SwaI Tth111I XcmI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AccI 779 GTCGAC GT^[AC][GT]AC
AhdI 340 GACTGGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 340 GACTGGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbvCI 81 CCTCAGC CCTCAGC(-5/-2)
BciVI 1080 GGATAC GTATCC(6/5)
BfaI 361 CTAG C^TAG
BglI 627 GCCTCAGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 357 AGATCT A^GATCT
BpmI 67 CTGGAG CTGGAG(16/14)
BsaJI 1066 CCATGG C^C[ACGT][ACGT]GG
BseRI 148 GAGGAG GAGGAG(10/8)
BsgI 758 GTGCAG GTGCAG(16/14)
BsiWI 133 CGTACG C^GTACG
BsmAI 348 GTCTC GTCTC(1/5)
BsmI 1013 GAATGC GAATGC(1/-1)
Bsp1407I 195 TGTACA T^GTACA
BsrDI 934 GCAATG GCAATG(2/0)
BsrFI 812 GCCGGT [GA]^CCGG[CT]
BsrGI 195 TGTACA T^GTACA
BstXI 286 CCAATTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 812 GCCGGT [GA]^CCGG[CT]
DsaI 1066 CCATGG C^C[GA][CT]GG
Eam1105I 340 GACTGGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT14I 1066 CCATGG C^C[AT][AT]GG
FauI 4 GCGGG CCCGC(4/6)
HapII 813 CCGG C^CGG
HgaI 177 GACGC GACGC(5/10)
HpaI 763 GTTAAC GTT^AAC
HpaII 813 CCGG C^CGG
MaeI 361 CTAG C^TAG
MslI 751 CATACAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 813 CCGG C^CGG
NcoI 1066 CCATGG C^CATGG
NlaIV 59 GGGGCC GG[ACGT]^[ACGT]CC
PleI 659 GAGTC GAGTC(4/5)
PvuII 617 CAGCTG CAG^CTG
SalI 779 GTCGAC G^TCGAC
SexAI 549 ACCAGGT A^CC[AT]GGT
SfaNI 1044 GCATC GCATC(5/9)
SfcI 426 CTATAG C^T[GA][CT]AG
SmlI 995 CTCAAG C^T[CT][GA]AG
SspBI 195 TGTACA T^GTACA
StyI 1066 CCATGG C^C[AT][AT]GG
TaqI 780 TCGA T^CGA
TthHB8I 780 TCGA T^CGA
XbaI 360 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 20 enzymes) #
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AcsI 688 AAATTC [GA]^AATT[CT]
916 GAATTT
ApoI 688 AAATTC [GA]^AATT[CT]
916 GAATTT
Asp700I 93 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
452 GAAGAGCTTC
AvaII 547 GGACC G^G[AT]CC
1074 GGTCC
BclI 512 TGATCA T^GATCA
798 TGATCA
BspHI 228 TCATGA T^CATGA
1035 TCATGA
CfoI 18 GCGC GCG^C
130 GCGC
EaeI 74 CGGCCA [CT]^GGCC[GA]
164 CGGCCA
FbaI 512 TGATCA T^GATCA
798 TGATCA
HhaI 18 GCGC GCG^C
130 GCGC
HinP1I 18 GCGC G^CGC
130 GCGC
MaeIII 391 GTCAC ^GT[ACGT]AC
490 GTCAC
MseI 370 TTAA T^TAA
764 TTAA
PflMI 613 CCAACAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
946 CCAGCCTTTGG
RcaI 228 TCATGA T^CATGA
1035 TCATGA
SapI 452 GAAGAGC GCTCTTC(1/4)
745 GAAGAGC
Tru9I 370 TTAA T^TAA
764 TTAA
Tsp45I 391 GTCAC ^GT[GC]AC
490 GTCAC
Van91I 613 CCAACAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
946 CCAGCCTTTGG
XmnI 93 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
452 GAAGAGCTTC
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# 3 OR MORE CUT ENZYMES ( 51 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI BbsI BbvI BpuAI BsiYI
BslI BsrI BssKI BstF5I BstNI
BstUI BstYI Cac8I Cfr13I DdeI
DpnII EarI EcoRII Fnu4HI FokI
HaeIII HincII HindII HinfI HphI
ItaI Ksp632I MboI MboII MflI
MnlI MspA1I MvaI MvnI MwoI
NdeII NlaIII RsaI Sau3AI Sau96I
ScrFI TfiI TseI Tsp509I TspRI
XhoII