Restriction map for
pFN21AE4044
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# NO CUT ENZYMES ( 148 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I AlwI AocI ApaLI ApoI
AscI AseI AsnI Asp718I AspEI
AspI AviII AvrII BamHI BbrPI
BbsI BbvCI BcgI BclI BfrI
BglI BglII BlnI BlpI Bpu1102I
BpuAI BsaAI BsaBI BsaI BsaWI
BseAI BsiEI BsiWI BsmBI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstXI BstYI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DraI DraIII
DrdI EagI Eam1105I EclXI Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT14I EcoT22I FbaI FseI FspI
HgaI HincII HindII HindIII HpaI
KpnI MaeIII MamI MfeI MflI
MluI MroI MseI MslI MunI
NaeI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SalI
SbfI ScaI SexAI SfiI SfuI
SgrAI SmlI SnaBI SpeI Sse8387I
SspBI SspI StyI SwaI TfiI
Tru9I Tsp45I Tsp509I Tth111I Van91I
XbaI XhoI XhoII
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# ONE CUT ENZYMES ( 50 enzymes) #
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AcyI 131 GGCGCC G[GA]^CG[CT]C
AlwNI 49 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 610 AGCGCT AGC^GCT
Asp700I 725 GAAACTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 219 GAGCAC G[AT]GC[AT]^C
BalI 323 TGGCCA TGG^CCA
BbeI 131 GGCGCC GGCGC^C
BciVI 453 GTATCC GTATCC(6/5)
BfaI 289 CTAG C^TAG
BpmI 481 CTGGAG CTGGAG(16/14)
BsaHI 131 GGCGCC G[GA]^CG[CT]C
BseRI 520 GAGGAG GAGGAG(10/8)
BsiHKAI 219 GAGCAC G[AT]GC[AT]^C
BsmAI 495 GAGAC GTCTC(1/5)
BsmI 568 GCATTC GAATGC(1/-1)
BsrBI 199 GAGCGG CCGCTC(-3/-3)
DdeI 467 CTCAG C^T[ACGT]AG
DpnII 276 GATC ^GATC
EaeI 323 TGGCCA [CT]^GGCC[GA]
Eco47III 610 AGCGCT AGC^GCT
Hin1I 131 GGCGCC G[GA]^CG[CT]C
HinfI 385 GAGTC G^A[ACGT]TC
KasI 131 GGCGCC G^GCGCC
KspI 573 CCGCGG CCGC^GG
MaeI 289 CTAG C^TAG
MaeII 681 ACGT A^CGT
MboI 276 GATC ^GATC
MluNI 323 TGGCCA TGG^CCA
MscI 323 TGGCCA TGG^CCA
NarI 131 GGCGCC GG^CGCC
NdeII 276 GATC ^GATC
NspI 306 GCATGC [GA]CATG^[CT]
PleI 385 GAGTC GAGTC(4/5)
PpuMI 589 AGGACCC [GA]G^G[AT]CC[CT]
PstI 343 CTGCAG CTGCA^G
SacII 573 CCGCGG CCGC^GG
SapI 88 GAAGAGC GCTCTTC(1/4)
Sau3AI 276 GATC ^GATC
SfaNI 638 GCATC GCATC(5/9)
SfcI 343 CTGCAG C^T[GA][CT]AG
SfoI 131 GGCGCC GGC^GCC
SmaI 233 CCCGGG CCC^GGG
SphI 306 GCATGC GCATG^C
StuI 668 AGGCCT AGG^CCT
TaqI 170 TCGA T^CGA
TthHB8I 170 TCGA T^CGA
XcmI 216 CCAGAGCACTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 233 CCCGGG C^CCGGG
XmaI 233 CCCGGG C^CCGGG
XmnI 725 GAAACTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 13 enzymes) #
###################################
AccII 360 CGCG CG^CG
574 CGCG
AvaI 76 CCCGAG C^[CT]CG[GA]G
233 CCCGGG
BsgI 101 CTGCAC GTGCAG(16/14)
262 CTGCAC
BsoBI 76 CCCGAG C^[CT]CG[GA]G
233 CCCGGG
BsrI 450 CCAGT ACTGG(1/-1)
624 CCAGT
BstUI 360 CGCG CG^CG
574 CGCG
EarI 729 CTCTTC CTCTTC(1/4)
88 GAAGAG
HphI 472 GGTGA GGTGA(8/7)
664 GGTGA
Ksp632I 729 CTCTTC CTCTTC(1/4)
88 GAAGAG
MboII 88 GAAGA GAAGA(8/7)
730 TCTTC
MvnI 360 CGCG CG^CG
574 CGCG
NlaIII 307 CATG CATG^
516 CATG
PvuII 279 CAGCTG CAG^CTG
478 CAGCTG
#########################################
# 3 OR MORE CUT ENZYMES ( 47 enzymes) #
#########################################
AciI AfaI AluI ApaI AvaII
BanI BanII BbvI BcnI BmyI
BsaJI BsiYI BslI BsmFI Bsp1286I
BssKI BstF5I BstNI Cac8I CfoI
Cfr13I DraII DsaI EcoO109I EcoRII
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HpaII ItaI
MnlI MspA1I MspI MvaI MwoI
NciI NlaIV RsaI Sau96I ScrFI
TseI TspRI