Restriction map for
pFN21AB0985
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# NO CUT ENZYMES ( 136 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AfaI AflII AflIII AgeI AhdI
Alw44I AlwNI AocI ApaLI AseI
AsnI Asp718I AspEI AspHI AviII
AvrII BalI BbrPI BbsI BbvCI
BcgI BciVI BclI BfrI BglII
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaBI BsaWI BseAI BsiHKAI BsiWI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrDI BsrGI BsrI BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstXI BstZ17I Bsu36I CelII ClaI
CpoI DraI DraIII DrdI Eam1105I
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HincII
HindII HindIII HpaI KpnI MaeII
MaeIII MamI MfeI MluI MluNI
MroI MscI MseI MslI MunI
NcoI NdeI NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflMI PinAI PleI PmaCI PmeI
PmlI PshBI Psp1406I PstI PvuI
RcaI RsaI RsrII SacI SalI
SapI SbfI ScaI SexAI SfcI
SfiI SfuI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI Tru9I Tsp45I Van91I XbaI
XhoI
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# ONE CUT ENZYMES ( 31 enzymes) #
###################################
AcsI 88 AAATTC [GA]^AATT[CT]
Aor51HI 102 AGCGCT AGC^GCT
ApaI 222 GGGCCC GGGCC^C
ApoI 88 AAATTC [GA]^AATT[CT]
AscI 700 GGCGCGCC GG^CGCGCC
Asp700I 537 GAACAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 187 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BfaI 340 CTAG C^TAG
BsaI 205 GAGACC GGTCTC(1/5)
BsgI 781 CTGCAC GTGCAG(16/14)
BsiEI 362 CGGCCG CG[GA][CT]^CG
BsmBI 610 GAGACG CGTCTC(1/5)
BsrBI 313 GAGCGG CCGCTC(-3/-3)
DdeI 598 CTCAG C^T[ACGT]AG
EagI 362 CGGCCG C^GGCCG
EclXI 362 CGGCCG C^GGCCG
Eco47III 102 AGCGCT AGC^GCT
Eco52I 362 CGGCCG C^GGCCG
HinfI 636 GAATC G^A[ACGT]TC
MaeI 340 CTAG C^TAG
NlaIII 8 CATG CATG^
PflFI 187 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 612 GACGCAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
TaqI 158 TCGA T^CGA
TfiI 636 GAATC G^A[AT]TC
Tsp509I 89 AATT ^AATT
TspRI 566 CAGTG CA[GC]TG[ACGT][ACGT]^
Tth111I 187 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 158 TCGA T^CGA
XcmI 137 CCAGGAAAAGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 537 GAACAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
BamHI 288 GGATCC G^GATCC
430 GGATCC
BspMI 615 GCAGGT ACCTGC(4/8)
687 GCAGGT
BstF5I 72 CATCC GGATG(2/0)
279 GGATG
BstYI 288 GGATCC [GA]^GATC[CT]
430 GGATCC
DpnII 289 GATC ^GATC
431 GATC
DraII 185 GGGACCC [GA]G^G[ACGT]CC[CT]
418 AGGTCCC
EarI 823 CTCTTC CTCTTC(1/4)
268 GAAGAG
EcoO109I 185 GGGACCC [GA]G^G[ACGT]CC[CT]
418 AGGTCCC
FokI 72 CATCC GGATG(9/13)
279 GGATG
Ksp632I 823 CTCTTC CTCTTC(1/4)
268 GAAGAG
MboI 289 GATC ^GATC
431 GATC
MflI 288 GGATCC [GA]^GATC[CT]
430 GGATCC
NaeI 232 GCCGGC GCC^GGC
458 GCCGGC
NdeII 289 GATC ^GATC
431 GATC
NgoMIV 232 GCCGGC G^CCGGC
458 GCCGGC
PpuMI 185 GGGACCC [GA]G^G[AT]CC[CT]
418 AGGTCCC
PvuII 199 CAGCTG CAG^CTG
520 CAGCTG
Sau3AI 289 GATC ^GATC
431 GATC
SgrAI 457 CGCCGGCG C[GA]^CCGG[CT]G
771 CACCGGCG
XhoII 288 GGATCC [GA]^GATC[CT]
430 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 71 enzymes) #
#########################################
AccII AciI AcyI AluI AlwI
AvaI AvaII BanI BanII BbeI
BbvI BcnI BglI BmyI BpmI
BsaHI BsaJI BseRI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BsrFI
BssHII BssKI BstNI BstUI Cac8I
CfoI Cfr10I Cfr13I DsaI EaeI
EcoRII EcoT14I FauI Fnu4HI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HpaII HphI ItaI KasI
KspI MboII MnlI MspA1I MspI
MvaI MvnI MwoI NarI NciI
NlaIV SacII Sau96I ScrFI SfaNI
SfoI SmaI StyI TseI XmaCI
XmaI