Restriction map for
pFN21AE3192
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# NO CUT ENZYMES ( 144 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AciI AclI AcsI AflII AgeI
AhdI Alw44I AlwI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AviII
AvrII BamHI BbeI BbrPI BbsI
BcgI BclI BfrI BglI BlnI
BlpI Bpu1102I BpuAI BsaAI BsaJI
BsaWI BseAI BseRI BsiEI BsiWI
BsmBI BsmI BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrDI BsrFI
BssHII Bst1107I BstBI BstUI BstXI
BstZ17I CelII CfoI Cfr10I ClaI
CpoI DraI DrdI DsaI EagI
Eam1105I EclXI Eco47III Eco52I EcoNI
EcoRI EcoRV EcoT14I EcoT22I FauI
FbaI FseI FspI HaeII HhaI
HinP1I HindIII HpaI KasI KpnI
KspI MfeI MluI MroI MspA1I
MunI MvnI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PflFI
PflMI PinAI PmaCI PmeI PmlI
PshBI Psp1406I PvuI PvuII RcaI
RsrII SacII SalI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
StyI SwaI Tth111I Van91I XbaI
XcmI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AcyI 698 GGCGTC G[GA]^CG[CT]C
AflIII 752 ACACGT A^C[GA][CT]GT
AocI 28 CCTCAGG CC^T[ACGT]AGG
AvaI 281 CTCGAG C^[CT]CG[GA]G
BalI 915 TGGCCA TGG^CCA
BanI 872 GGCACC G^G[CT][GA]CC
BbvCI 40 GCTGAGG CCTCAGC(-5/-2)
BciVI 1205 GGATAC GTATCC(6/5)
BcnI 401 CCCGG CC^[GC]GG
BsaBI 588 GATGTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 698 GGCGTC G[GA]^CG[CT]C
BsgI 657 GTGCAG GTGCAG(16/14)
BsmFI 1120 GTCCC GGGAC(10/14)
BsoBI 281 CTCGAG C^[CT]CG[GA]G
BssSI 785 CTCGTG CACGAG(-5/-1)
BstAPI 1252 GCAGTCAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1177 GGTCACC G^GT[ACGT]ACC
BstF5I 149 GGATG GGATG(2/0)
BstPI 1177 GGTCACC G^GT[ACGT]ACC
Bsu36I 28 CCTCAGG CC^T[ACGT]AGG
DraII 581 AGGTCCT [GA]G^G[ACGT]CC[CT]
DraIII 874 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 915 TGGCCA [CT]^GGCC[GA]
Eco81I 28 CCTCAGG CC^T[ACGT]AGG
EcoO109I 581 AGGTCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 1177 GGTCACC G^GT[ACGT]ACC
FokI 149 GGATG GGATG(9/13)
HapII 402 CCGG C^CGG
HgaI 699 GCGTC GACGC(5/10)
Hin1I 698 GGCGTC G[GA]^CG[CT]C
HincII 930 GTTGAC GT[CT]^[GA]AC
HindII 930 GTTGAC GT[CT]^[GA]AC
HpaII 402 CCGG C^CGG
MamI 588 GATGTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 915 TGGCCA TGG^CCA
MscI 915 TGGCCA TGG^CCA
MslI 561 CATATCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 402 CCGG C^CGG
NciI 401 CCCGG CC^[GC]GG
NlaIV 872 GGCACC GG[ACGT]^[ACGT]CC
PaeR7I 281 CTCGAG C^TCGAG
PleI 511 GAGTC GAGTC(4/5)
PpuMI 581 AGGTCCT [GA]G^G[AT]CC[CT]
PshAI 1248 GACTGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SapI 943 GAAGAGC GCTCTTC(1/4)
SfaNI 994 GATGC GCATC(5/9)
SspI 884 AATATT AAT^ATT
StuI 1333 AGGCCT AGG^CCT
XhoI 281 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 26 enzymes) #
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AlwNI 35 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
726 CAGAAGCTG
AspHI 140 GAGCTC G[AT]GC[AT]^C
323 GAGCTC
AvaII 582 GGTCC G^G[AT]CC
624 GGACC
BanII 140 GAGCTC G[GA]GC[CT]^C
323 GAGCTC
BglII 111 AGATCT A^GATCT
471 AGATCT
BmyI 140 GAGCTC G[AGT]GC[ACT]^C
323 GAGCTC
BsaI 683 GAGACC GGTCTC(1/5)
404 GGTCTC
BsiHKAI 140 GAGCTC G[AT]GC[AT]^C
323 GAGCTC
Bsp1286I 140 GAGCTC G[AGT]GC[ACT]^C
323 GAGCTC
BstYI 111 AGATCT [GA]^GATC[CT]
471 AGATCT
Cac8I 117 GCAGGC GC[ACGT]^[ACGT]GC
706 GCCTGC
Cfr13I 582 GGTCC G^G[ACGT]CC
624 GGACC
EarI 289 CTCTTC CTCTTC(1/4)
943 GAAGAG
Ksp632I 289 CTCTTC CTCTTC(1/4)
943 GAAGAG
MaeII 754 ACGT A^CGT
966 ACGT
MflI 111 AGATCT [GA]^GATC[CT]
471 AGATCT
MwoI 642 GCAGTTGTCGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
1252 GCAGTCAGTGC
NlaIII 919 CATG CATG^
1150 CATG
PstI 115 CTGCAG CTGCA^G
1250 CTGCAG
SacI 140 GAGCTC GAGCT^C
323 GAGCTC
Sau96I 582 GGTCC G^G[ACGT]CC
624 GGACC
SmlI 281 CTCGAG C^T[CT][GA]AG
541 CTTGAG
TaqI 195 TCGA T^CGA
282 TCGA
Tsp509I 184 AATT ^AATT
999 AATT
TthHB8I 195 TCGA T^CGA
282 TCGA
XhoII 111 AGATCT [GA]^GATC[CT]
471 AGATCT
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# 3 OR MORE CUT ENZYMES ( 39 enzymes) #
#########################################
AfaI AluI BbvI BfaI BpmI
BsiYI BslI BsmAI Bsp1407I BsrGI
BsrI BssKI BstNI DdeI DpnII
EcoRII Fnu4HI HaeIII HinfI HphI
ItaI MaeI MaeIII MboI MboII
MnlI MseI MvaI NdeII RsaI
Sau3AI ScrFI SfcI SspBI TfiI
Tru9I TseI Tsp45I TspRI