Restriction map for
pFN21AE3062
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# NO CUT ENZYMES ( 175 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AspI AvaII
AviII AvrII BalI BamHI BanI
BanII BbeI BbrPI BbsI BcgI
BciVI BclI BfrI BglI BglII
BlnI BlpI BmyI Bpu1102I BpuAI
BsaAI BsaBI BsaHI BsaWI BseAI
BseRI BsiEI BsiHKAI BsiWI BsmBI
Bsp1286I BspDI BspEI BspHI BspMI
BsrDI BsrFI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstUI BstXI
BstZ17I Bsu36I CelII Cfr10I Cfr13I
ClaI CpoI DraI DraII DraIII
EaeI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRI EcoRV EcoT14I EcoT22I
FbaI FseI FspI HaeII HaeIII
HgaI Hin1I HincII HindII HindIII
HpaI KasI KpnI Ksp632I KspI
MamI MfeI MluI MluNI MroI
MscI MspA1I MunI MvnI NaeI
NarI NcoI NdeI NgoMIV NheI
NlaIV NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI PvuII
RcaI RsrII SacI SacII SalI
SapI Sau96I SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmlI
SnaBI SpeI SphI Sse8387I SspI
StuI StyI SwaI Tsp45I TspRI
Tth111I Van91I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AcsI 548 AAATTT [GA]^AATT[CT]
AfaI 343 GTAC GT^AC
AflIII 306 ACATGT A^C[GA][CT]GT
ApoI 548 AAATTT [GA]^AATT[CT]
AvaI 100 CCCGGG C^[CT]CG[GA]G
BbvCI 467 CCTCAGC CCTCAGC(-5/-2)
BbvI 105 GCTGC GCAGC(8/12)
BpmI 160 CTGGAG CTGGAG(16/14)
BsaI 4 GAGACC GGTCTC(1/5)
BsgI 73 CTGCAC GTGCAG(16/14)
BsoBI 100 CCCGGG C^[CT]CG[GA]G
Bsp1407I 342 TGTACA T^GTACA
BspLU11I 306 ACATGT A^CATGT
BsrBI 217 GAGCGG CCGCTC(-3/-3)
BsrGI 342 TGTACA T^GTACA
BsrI 21 CCAGT ACTGG(1/-1)
BstYI 14 GGATCT [GA]^GATC[CT]
CfoI 108 GCGC GCG^C
DrdI 124 GACCCCACAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 53 CCGTGG C^C[GA][CT]GG
Fnu4HI 105 GCTGC GC^[ACGT]GC
HhaI 108 GCGC GCG^C
HinP1I 108 GCGC G^CGC
HphI 83 TCACC GGTGA(8/7)
ItaI 105 GCTGC GC^[ACGT]GC
MaeII 402 ACGT A^CGT
MaeIII 409 GTTAC ^GT[ACGT]AC
MflI 14 GGATCT [GA]^GATC[CT]
MslI 379 CATAAATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NlaIII 307 CATG CATG^
NspI 306 ACATGT [GA]CATG^[CT]
PleI 46 GACTC GAGTC(4/5)
RsaI 343 GTAC GT^AC
SfaNI 478 GATGC GCATC(5/9)
SfcI 190 CTACAG C^T[GA][CT]AG
SmaI 100 CCCGGG CCC^GGG
SspBI 342 TGTACA T^GTACA
TaqI 167 TCGA T^CGA
TfiI 496 GAATC G^A[AT]TC
TseI 105 GCTGC G^C[AT]GC
Tsp509I 549 AATT ^AATT
TthHB8I 167 TCGA T^CGA
XcmI 128 CCACAGTCGGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 14 GGATCT [GA]^GATC[CT]
XmaCI 100 CCCGGG C^CCGGG
XmaI 100 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AlwI 14 GGATC GGATC(4/5)
182 GGATC
BfaI 322 CTAG C^TAG
370 CTAG
BsiYI 127 CCCACAGTCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
153 CCGCCTGCTGG
BslI 127 CCCACAGTCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
153 CCGCCTGCTGG
BsmAI 4 GAGAC GTCTC(1/5)
518 GAGAC
BsmI 640 GAATGC GAATGC(1/-1)
606 GCATTC
BstAPI 59 GCAAGTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
472 GCAAAGGATGC
BstF5I 477 GGATG GGATG(2/0)
561 GGATG
BstNI 87 CCAGG CC^[AT]GG
632 CCAGG
Cac8I 118 GCCTGC GC[ACGT]^[ACGT]GC
155 GCCTGC
EcoRII 87 CCAGG ^CC[AT]GG
632 CCAGG
FokI 477 GGATG GGATG(9/13)
561 GGATG
HapII 101 CCGG C^CGG
172 CCGG
HinfI 46 GACTC G^A[ACGT]TC
496 GAATC
HpaII 101 CCGG C^CGG
172 CCGG
MaeI 322 CTAG C^TAG
370 CTAG
MseI 536 TTAA T^TAA
578 TTAA
MspI 101 CCGG C^CGG
172 CCGG
MvaI 87 CCAGG CC^[AT]GG
632 CCAGG
MwoI 59 GCAAGTCCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
472 GCAAAGGATGC
Tru9I 536 TTAA T^TAA
578 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 16 enzymes) #
#########################################
AciI AluI BcnI BsaJI BsmFI
BssKI DdeI DpnII FauI MboI
MboII MnlI NciI NdeII Sau3AI
ScrFI