Restriction map for
pFN21AE2149
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# NO CUT ENZYMES ( 114 enzymes) #
##################################
AatII AccIII AclI AcsI AflII
AgeI AhdI AocI Aor51HI ApoI
AscI AseI AsnI Asp700I AspEI
AspI AviII AvrII BamHI BbsI
BciVI BclI BfaI BfrI BglII
BlnI BpuAI BsaBI BsaWI BseAI
BsiWI BsmBI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrDI BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstXI BstZ17I Bsu36I ClaI CpoI
DraI DrdI Eam1105I EarI Eco47III
Eco81I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HindIII HpaI
Ksp632I KspI MaeI MamI MfeI
MluI MroI MseI MunI NcoI
NdeI NheI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmeI
PpuMI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacI SacII SapI
ScaI SexAI SfiI SfuI SgrAI
SmlI SnaBI SpeI SphI SspBI
SspI SwaI TfiI Tru9I Tsp509I
Tth111I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 37 enzymes) #
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Acc65I 248 GGTACC G^GTACC
AccI 409 GTCGAC GT^[AC][GT]AC
AfaI 249 GTAC GT^AC
Alw44I 1089 GTGCAC G^TGCAC
ApaLI 1089 GTGCAC G^TGCAC
Asp718I 248 GGTACC G^GTACC
BalI 1268 TGGCCA TGG^CCA
BbeI 875 GGCGCC GGCGC^C
BbvCI 1576 CCTCAGC CCTCAGC(-5/-2)
BsaI 429 GGTCTC GGTCTC(1/5)
BsmAI 430 GTCTC GTCTC(1/5)
BsmI 284 GAATGC GAATGC(1/-1)
BsrBI 43 GAGCGG CCGCTC(-3/-3)
BsrFI 1060 GCCGGC [GA]^CCGG[CT]
Cfr10I 1060 GCCGGC [GA]^CCGG[CT]
DraIII 556 CACGCTGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoNI 456 CCTGGACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HincII 409 GTCGAC GT[CT]^[GA]AC
HindII 409 GTCGAC GT[CT]^[GA]AC
KasI 875 GGCGCC G^GCGCC
KpnI 248 GGTACC GGTAC^C
MluNI 1268 TGGCCA TGG^CCA
MscI 1268 TGGCCA TGG^CCA
NaeI 1060 GCCGGC GCC^GGC
NarI 875 GGCGCC GG^CGCC
NgoMIV 1060 GCCGGC G^CCGGC
NotI 859 GCGGCCGC GC^GGCCGC
NspI 1448 ACATGC [GA]CATG^[CT]
PflMI 555 CCACGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RsaI 249 GTAC GT^AC
SalI 409 GTCGAC G^TCGAC
SbfI 92 CCTGCAGG CCTGCA^GG
SfoI 875 GGCGCC GGC^GCC
Sse8387I 92 CCTGCAGG CCTGCA^GG
StuI 37 AGGCCT AGG^CCT
Van91I 555 CCACGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 395 CCAAGGATCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 24 enzymes) #
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AcyI 177 GGCGTC G[GA]^CG[CT]C
875 GGCGCC
AflIII 290 ACGTGT A^C[GA][CT]GT
782 ACGTGT
AlwI 399 GGATC GGATC(4/5)
506 GGATC
AspHI 440 GTGCTC G[AT]GC[AT]^C
1089 GTGCAC
AvaII 28 GGACC G^G[AT]CC
1716 GGACC
BcgI 647 GCACCAGCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
894 GCAGCTGGTTCG
BsaHI 177 GGCGTC G[GA]^CG[CT]C
875 GGCGCC
BseRI 271 GAGGAG GAGGAG(10/8)
1252 GAGGAG
BsgI 1409 CTGCAC GTGCAG(16/14)
231 GTGCAG
BsiEI 626 CGGCCG CG[GA][CT]^CG
860 CGGCCG
BsiHKAI 440 GTGCTC G[AT]GC[AT]^C
1089 GTGCAC
BstAPI 138 GCAGAGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
736 GCAGCAGCTGC
BstYI 399 GGATCT [GA]^GATC[CT]
506 GGATCT
EagI 626 CGGCCG C^GGCCG
860 CGGCCG
EclXI 626 CGGCCG C^GGCCG
860 CGGCCG
Eco52I 626 CGGCCG C^GGCCG
860 CGGCCG
EcoT14I 395 CCAAGG C^C[AT][AT]GG
1271 CCAAGG
Hin1I 177 GGCGTC G[GA]^CG[CT]C
875 GGCGCC
HinfI 274 GAGTC G^A[ACGT]TC
535 GACTC
MflI 399 GGATCT [GA]^GATC[CT]
506 GGATCT
MslI 289 CACGTGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
834 CACCCCTGTG
PleI 535 GACTC GAGTC(4/5)
274 GAGTC
StyI 395 CCAAGG C^C[AT][AT]GG
1271 CCAAGG
XhoII 399 GGATCT [GA]^GATC[CT]
506 GGATCT
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# 3 OR MORE CUT ENZYMES ( 83 enzymes) #
#########################################
AccII AciI AluI AlwNI ApaI
AvaI BanI BanII BbrPI BbvI
BcnI BglI BlpI BmyI BpmI
Bpu1102I BsaAI BsaJI BsiYI BslI
BsmFI BsoBI Bsp1286I BspMI BsrI
BssKI BstF5I BstNI BstUI Cac8I
CelII CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EcoO109I EcoRII
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI HinP1I HpaII
HphI ItaI MaeII MaeIII MboI
MboII MnlI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PmaCI PmlI PstI PvuII
Sau3AI Sau96I ScrFI SfaNI SfcI
SmaI TaqI TseI Tsp45I TspRI
TthHB8I XmaCI XmaI