Restriction map for
pFN21AE3086
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# NO CUT ENZYMES ( 134 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AfaI AflII AhdI Aor51HI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AviII AvrII
BalI BamHI BbeI BbrPI BbsI
BbvCI BciVI BclI BfaI BfrI
BglII BlnI BlpI Bpu1102I BpuAI
BsaAI BsaBI BsaI BseAI BseRI
BsgI BsiWI BsmI Bsp1407I BspEI
BspHI BspLU11I BspMI BsrBI BsrGI
BssHII BssSI Bst1107I BstBI BstEII
BstF5I BstPI BstYI BstZ17I CelII
CpoI DraI DrdI Eam1105I Eco47III
EcoNI EcoO65I EcoRI EcoRV EcoT14I
EcoT22I FbaI FokI FseI FspI
HindIII HpaI KasI KpnI MaeI
MamI MfeI MflI MluI MluNI
MroI MscI MseI MunI NarI
NcoI NdeI NheI NotI NruI
NsiI NspI NspV PacI PflFI
PleI PmaCI PmeI PmlI PpuMI
PshBI Psp1406I PvuI RcaI RsaI
RsrII SacI SalI SbfI ScaI
SexAI SfiI SfoI SfuI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI StyI SwaI Tru9I Tsp509I
Tth111I XbaI XhoII XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AflIII 551 ACGTGT A^C[GA][CT]GT
AgeI 559 ACCGGT A^CCGGT
Alw44I 555 GTGCAC G^TGCAC
AlwNI 331 CAGAACCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 255 CCTGAGG CC^T[ACGT]AGG
ApaI 658 GGGCCC GGGCC^C
ApaLI 555 GTGCAC G^TGCAC
BanII 658 GGGCCC G[GA]GC[CT]^C
BcgI 605 GCACCCCCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BpmI 60 CTCCAG CTGGAG(16/14)
BsaWI 559 ACCGGT [AT]^CCGG[AT]
BsiEI 87 CGGCCG CG[GA][CT]^CG
BsmBI 484 CGTCTC CGTCTC(1/5)
BspDI 715 ATCGAT AT^CGAT
BsrDI 590 GCAATG GCAATG(2/0)
BstAPI 437 GCACCAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 468 CCAGCGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 255 CCTGAGG CC^T[ACGT]AGG
ClaI 715 ATCGAT AT^CGAT
DdeI 256 CTGAG C^T[ACGT]AG
DraIII 634 CACGGCGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 87 CGGCCG C^GGCCG
EarI 700 CTCTTC CTCTTC(1/4)
EclXI 87 CGGCCG C^GGCCG
Eco52I 87 CGGCCG C^GGCCG
Eco81I 255 CCTGAGG CC^T[ACGT]AGG
HaeII 696 GGCGCT [GA]GCGC^[CT]
HincII 508 GTCAAC GT[CT]^[GA]AC
HindII 508 GTCAAC GT[CT]^[GA]AC
HinfI 135 GAATC G^A[ACGT]TC
Ksp632I 700 CTCTTC CTCTTC(1/4)
PaeR7I 48 CTCGAG C^TCGAG
PflMI 633 CCACGGCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 559 ACCGGT A^CCGGT
PshAI 532 GACCAGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 412 CTGCAG CTGCA^G
SapI 699 GCTCTTC GCTCTTC(1/4)
SfcI 412 CTGCAG C^T[GA][CT]AG
SgrAI 558 CACCGGTG C[GA]^CCGG[CT]G
SmlI 48 CTCGAG C^T[CT][GA]AG
TfiI 135 GAATC G^A[AT]TC
Van91I 633 CCACGGCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 48 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 31 enzymes) #
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AccII 192 CGCG CG^CG
577 CGCG
AcyI 42 GGCGTC G[GA]^CG[CT]C
482 GGCGTC
AluI 199 AGCT AG^CT
359 AGCT
AlwI 168 GATCC GGATC(4/5)
713 GGATC
AspHI 555 GTGCAC G[AT]GC[AT]^C
565 GTGCTC
BglI 22 GCCCTGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
575 GCCGCGGTGGC
BsaHI 42 GGCGTC G[GA]^CG[CT]C
482 GGCGTC
BsiHKAI 555 GTGCAC G[AT]GC[AT]^C
565 GTGCTC
BsmAI 707 GAGAC GTCTC(1/5)
485 GTCTC
BstUI 192 CGCG CG^CG
577 CGCG
CfoI 471 GCGC GCG^C
697 GCGC
DpnII 168 GATC ^GATC
714 GATC
HgaI 43 GCGTC GACGC(5/10)
483 GCGTC
HhaI 471 GCGC GCG^C
697 GCGC
Hin1I 42 GGCGTC G[GA]^CG[CT]C
482 GGCGTC
HinP1I 471 GCGC G^CGC
697 GCGC
KspI 191 CCGCGG CCGC^GG
576 CCGCGG
MboI 168 GATC ^GATC
714 GATC
MboII 683 TCTTC GAAGA(8/7)
701 TCTTC
MvnI 192 CGCG CG^CG
577 CGCG
NdeII 168 GATC ^GATC
714 GATC
NlaIII 172 CATG CATG^
303 CATG
PvuII 198 CAGCTG CAG^CTG
358 CAGCTG
SacII 191 CCGCGG CCGC^GG
576 CCGCGG
Sau3AI 168 GATC ^GATC
714 GATC
SmaI 220 CCCGGG CCC^GGG
735 CCCGGG
TaqI 49 TCGA T^CGA
716 TCGA
TthHB8I 49 TCGA T^CGA
716 TCGA
XcmI 62 CCAGCCCCCTTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
440 CCAGATGCCAGGTGG
XmaCI 220 CCCGGG C^CCGGG
735 CCCGGG
XmaI 220 CCCGGG C^CCGGG
735 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 50 enzymes) #
#########################################
AciI AvaI AvaII BanI BbvI
BcnI BmyI BsaJI BsiYI BslI
BsmFI BsoBI Bsp1286I BsrFI BsrI
BssKI BstNI Cac8I Cfr10I Cfr13I
DraII DsaI EaeI EcoO109I EcoRII
FauI Fnu4HI HaeIII HapII HpaII
HphI ItaI MaeII MaeIII MnlI
MslI MspA1I MspI MvaI MwoI
NaeI NciI NgoMIV NlaIV Sau96I
ScrFI SfaNI TseI Tsp45I TspRI